Bacterial communities associated with the gut of tomato fruit borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) based on Illumina Next-Generation Sequencing

Ranjith, M.; ManiChellappan,; Harish, E R; Girija, D.; Nazeem, P.

doi:10.1016/j.aspen.2016.03.007

Cited by 21 publications

(29 citation statements)

References 57 publications

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“…Phylum Actinobacteria and uncultured Bacteria were the most transcriptionally active, whereas Proteobacteria and Firmicutes accounted for <2% (Table ). These results are in accordance with a recent study that used high‐throughput sequencing of 16S rDNA to analyse the gut microbiota of late‐instar field‐captured H. armigera larvae, where Actinobacteria was the dominant phylum followed by Proteobacteria and Firmicutes (Ranjith et al, ). Moreover, in another study that also used next‐generation sequencing of 16S rDNA, a very high proportion of the S. litura larval gut microbiota corresponded to uncultured Bacteria (Bapatla et al, ).…”

Section: Discussionsupporting

confidence: 91%

“…Even though this species has not been previously reported in insects, given its ability to degrade hemicellulose and xylan (Carreto et al, ) and its high level of activity ostensibly related to carbohydrate metabolism in our sample, we suggest that it was participating in the digestive process by facilitating the breakdown of organic substrates in the foliage, and thus playing an important role in host nutrition. Similarly, a recent study that analysed gut microbiota in H. armigera found that the dominant species was also an Actinobacteria which had not been described previously in insect guts and was capable of metabolizing cellulose and xylan (Ranjith et al, ). In another study which studied the gut metagenome of P. xylostella , carbohydrate metabolism was also found to be one of the most enriched functions (Xia et al, ), but these authors suggested that Enterobacter spp.…”

Section: Discussionmentioning

confidence: 76%

“…To conclude, various studies have analysed the gut microbiota of insects, and a high proportion of these have used next‐generation sequencing approaches. These studies have mostly focused on the taxonomic composition of the microbiome through analysis of the rRNA gene, and some have also inferred the functional profile (Bapatla et al, ; Ranjith et al, ; Tang et al, ), but very few have determined the metabolically active components (Chen et al, ; Shao et al, ). Moreover, in most of these studies the focus has been on the bacterial community, with little or no integration with the rest of the non‐bacterial microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, and considering that resident microbiota have great potential to be used as improved control methods (Douglas, ), it is of paramount importance to gain insight into the composition and functional role of this insect's gut microbiota. Furthermore, studies describing the microbiome in lepidopterans are few and have mostly focused on Spodoptera littoralis (Boisduval; Chen et al, ; Shao, Arias‐Cordero, Guo, Bartram, & Boland, ; Shao et al, ; Tang et al, ), Plutella xylostella (L.) (Indiragandhi et al, ; Li, Jin, Shi, & Li, ; Xia et al, ), Helicoverpa armigera (Hübner; Priya, Ojha, Kajla, Raj, & Rajagopal, ; Ranjith, ManiChellappan, Harish, Girija, & Nazeem, ), Plodia interpunctella (Hübner; Mereghetti, Chouaia, Limonta, Locatelli, & Montagna, ; Montagna et al, ), Anticarsia gemmatalis (Hübner; Visôtto et al, ), Spodoptera litura (Fabr. ; Bapatla, Singh, Yeddula, & Patil, ) and Lymantria dispar (L.) (Broderick, Raffa, Goodman, & Handelsman, ).…”

Section: Introductionmentioning

confidence: 99%

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Gut microbiota ofSpodoptera frugiperda(J.E. Smith) larvae as revealed by metatranscriptomic analysis

Rozadilla

Cabrera

Virla

et al. 2020

J Applied Entomology

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The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith; Lepidoptera: Noctuidae), is considered one of the most harmful pests in corn, rice and cotton crops and has developed resistance to the most widespread methods used to control it, chemical insecticides and transgenic crops based on Bt toxins. On the other hand, gut microbiota plays an important role in the insect's fitness, and metatranscriptomic analyses of the insect host are an invaluable tool for identifying new pest control targets. Notwithstanding, there are extremely few reports on the functionally active profile of the gut microbiota in insects, especially in lepidopterans. Moreover, most studies have only described the bacterial community even though all the components of the microbiota, Bacteria, Archaea, fungi, protozoa and viruses, influence different physiological aspects of the insect host. This is the first time an unbiased and comprehensive metatranscriptomic approach has been used to describe the taxonomic and functional profile of the gut microbiota of S. frugiperda larvae, both bacterial and non‐bacterial. We identified novel and very active components which were putatively playing an important nutritional role, such as ammonia‐oxidizing Archaea and Bacteria, and a xylan‐ and hemicellulose‐degrading Actinobacteria. This analysis also allowed us to identify potential biocontrol agents.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gut microbiota ofSpodoptera frugiperda(J.E. Smith) larvae as revealed by metatranscriptomic analysis

Rozadilla

Cabrera

Virla

et al. 2020

J Applied Entomology

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“…The gut of S. litura and S. obliqua can be viewed as bioreactors, in which various biogeochemical, detoxification and biodegradation activities are going on simultaneously and the metagenome analyses from this investigation illustrated taxonomic, functional and metabolic diversity. Taxonomic hierarchies abundance had not followed the trend, indicated earlier by Ranjith et al [7] reported Actinobacteria is the most dominant group followed by proteobacteria and Firmicutes from entire gut metagenome of H. armigera larvae. Bacterial community in midgut of H. armigera [16], gut, and reproductive organs of both male and female fruit fly, Bactrocera minax, gut of ground beetles [17], the Lutzomiya sand fly [18], and desert locust, Schistocerca gregaria [19] illustrated proteobacteria is the dominant phyla followed by Firmicutes and Actinobacteria groups.…”

Section: Discussionmentioning

confidence: 74%

Annotation of gut bacterial taxonomic and functional diversity in Spodoptera litura and Spilosoma obliqua

et al. 2018

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The insect gut has been the house of many taxonomically and physiologically diverse groups of microbial colonizers as symbionts and commensals, which are evolving to support the physiological requirement of insects. Lepidoptera is one of the important family of class hexapoda, comprising agriculture insect pest Spodoptera litura and Spilosoma obliqua. Information on gut microbiota and their functional role in these insects was meager to elucidate the wide-ranging survivalist mechanisms. In this context, we analyzed the composition, diversity and functional role of gut bacteria in S. litura and S. obliqua collected from soybean and sunflower crops, respectively, using Next Generation Sequencing of 16S rRNA. A total of 3427 and 206 Operation Taxonomic Units (OTUs) were identified in S. litura and S. obliqua gut metagenome, respectively. Highest number of sequences were annotated to unclassified bacteria (34%), followed by Proteobacteria (27%), and Chlorobi (14%) in S. litura, while S. obliqua has significant representation of Firmicutes (48%), followed by Bacteroidetes (20%), and unclassified bacteria (11%). Functionality of both metagenomes revealed, high abundance of ammonia oxidizers (20.1 58.0%) followed by relative abundance of detoxifying processes - dehalogenation (17.4-41.2%) and aromatic hydrocarbons degradation (1.1-3.1%). This study highlights the significance of the inherent microbiome of two defoliators in shaping the metagenome for nutrition and detoxifying the chemical molecules, and opens an avenue for exploring role of insect gut bacteria in host selection, metabolic endurance of insecticides and synergistic or agonistic mechanisms inside gut of insects feeding on insect-resistant biotech crops.

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Bacterial diversity of Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) from different geographical conditions in China

Guo

Peng

Wang

et al. 2021

Arch Insect Biochem Physiol

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Insects harbor numerous endosymbionts, including bacteria, fungi, yeast, and viruses, which could affect the ecology and behavior of their hosts. However, data regarding the effect of environmental factors on endosymbiotic bacteria of Leptocybe invasa (Hymenoptera: Eulophidae) are quite rare. In this study, we assessed the diversity of endosymbiotic bacteria of L. invasa from 10 different geographic populations collected across China through the Illumina MiSeq platform. A total of 547 OTUs were generated, which were annotated into 19 phyla, 33 classes, 75 orders, 137 families, and 274 genera. The dominant bacteria detected in L. invasa were Rickettsia, and Pantoea, Enterobacter, Pseudomonas, Acinetobacter, and Bacillus were also annotated among each population. Nevertheless, the endosymbiotic bacterial abundance and diversity varied among different populations, which was related to the local climate (annual mean high temperature).The bacterial function prediction analysis showed that these endosymbiotic bacteria were concentrated in metabolism, such as carbohydrate, amino acid, and energy metabolism. Overall, the results provide a comprehensive description of the endosymbiotic bacteria in 10 different populations of an important eucalyptus pest L. invasa, and

show abstract

Bacterial communities associated with the gut of tomato fruit borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) based on Illumina Next-Generation Sequencing

Cited by 21 publications

References 57 publications

Gut microbiota ofSpodoptera frugiperda(J.E. Smith) larvae as revealed by metatranscriptomic analysis

Gut microbiota ofSpodoptera frugiperda(J.E. Smith) larvae as revealed by metatranscriptomic analysis

Annotation of gut bacterial taxonomic and functional diversity in Spodoptera litura and Spilosoma obliqua

Bacterial diversity of Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) from different geographical conditions in China

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