Bacterial communities associated with scyphomedusae at Helgoland Roads

Abstract

Different modes of asexual and sexual reproduction are typical for the life history of scyphozoans, and numerous studies have focused on general life history distribution, reproductive strategies, strobilation-inducing factors, growth rates, and predatory effects of medusae. However, bacteria associated with different life stages of Scyphozoa have received less attention. In this study, bacterial communities associated with different body compartments and different life stages of two common scyphomedusae (Cyanea lamarckii and Chrysaora hysoscella) were analyzed via automated ribosomal intergenic spacer analysis (ARISA). We found that the bacterial community associated with these two species showed speciesspecific structuring. In addition, we observed significant differences between the bacterial communities associated with the umbrella and other body compartments (gonads and tentacles) of the two scyphomedusan species. Bacterial community structure varied from the early planula to the polyp and adult medusa stages. We also found that the free-living and particle-associated bacterial communities associated with different food sources had no impact on the bacterial community associated with fed polyps. Keywords Cyanea lamarckii. Chrysaora hysoscella. Automated ribosomal intergenic spacer analysis (ARISA). Scyphozoan body compartments. Scyphozoan life stages. German Bight

Jellyfish-Associated Microbiome in the Marine Environment: Exploring Its Biotechnological Potential

“…Within Semaeostomeae members of Ulmaridae ( Aurelia aurita , or moon jellyfish, [ 35 , 36 , 37 ]), Cyaneidae ( Cyanea capillata , known as lion’s mane jellyfish, and Cyanea lamarckii , or blue fire jellyfish [ 38 , 39 ]) and Pelagiidae ( Pelagia noctiluca , known as mauve stinger [ 33 ], Chrysaora plocamia , a South American sea nettle [ 40 ], and Chrysaora hysoscella , compass jellyfish [ 39 ]) were studied for their associated microbial communities.…”

“…The second study on C. lamarckii was published by Hao et al [ 39 ]. This study provides valuable insights into the microbial community associated with different Cyanea body parts, revealing significant differences between bacterial communities associated with the umbrella and other parts, i.e., gonads, mouth, arm, and tentacles.…”

“…Furthermore, jellyfish analyzed in this study were collected using 500 µm mesh trawls, introducing potential cross-contamination with microbes associated with other biological material accumulated in the cod end of the net. The data on microbial community associated with C. lamarckii tentacles from the two available studies cannot be compared, unfortunately, since the taxonomic composition of the microbial community was not analyzed by Hao et al [ 39 ] and the data obtained by DGGE [ 38 ] and automated ribosomal intergenic spacer analysis (ARISA) [ 39 ] are not comparable due to the different resolutions of these two fingerprinting techniques.…”

“…Another representative of the Chrysaora genus, Chrysaora hysoscella , collected in the German Bight was studied in Reference [ 39 ]. The pluses and minuses of this study are discussed above (see the paragraph on Cyanea lamarckii ).…”

Drivers of Bacterial α- and β-Diversity Patterns and Functioning in Subsurface Hadal Sediments

“…Despite several studies have proved that fingerprinting techniques, and especially ARISA, provide results consistent with those obtained using massive sequencing approaches in the assessment of bacterial diversity patterns (Schauer et al, 2010; Schöttner et al, 2011; Ristova et al, 2015; Jessen et al, 2017; Lindström et al, 2018), the contribution of “rare” members of the benthic assemblages could be underestimated to favor the detection of the more abundant members (Bent et al, 2007; Jones et al, 2012). Despite these limitations, fingerprinting techniques are still largely utilized in ecological studies aimed at investigating patterns and drivers of bacterial diversity on a wide variety of environmental samples (Billard et al, 2015; Cram et al, 2015; Fonti et al, 2015; Fuhrman et al, 2015; Witt et al, 2017; Lindström et al, 2018; Hao et al, 2019; Molari et al, 2019; Niederberger et al, 2019). Therefore, results reported here can contribute to expand our knowledge about the patterns of bacterial diversity in hadal sediments and provide new cues for future investigations in such remote ecosystems.…”