Bacterial Communities Associated with Atherosclerotic Plaques from Russian Individuals with Atherosclerosis

Abstract

Atherosclerosis is considered a chronic disease of the arterial wall and is the major cause of severe disease and death among individuals all over the world. Some recent studies have established the presence of bacteria in atherosclerotic plaque samples and suggested their possible contribution to the development of cardiovascular disease. The main objective of this preliminary pilot study was to better understand the bacterial diversity and abundance in human atherosclerotic plaques derived from common carotid arteries of individuals with atherosclerosis (Russian nationwide group) and contribute towards the further identification of a main group of atherosclerotic plaque bacteria by 454 pyrosequencing their 16S ribosomal RNA (16S rRNA) genes. The applied approach enabled the detection of bacterial DNA in all atherosclerotic plaques. We found that distinct members of the order Burkholderiales were present at high levels in all atherosclerotic plaques obtained from patients with atherosclerosis with the genus Curvibacter being predominant in all plaque samples. Moreover, unclassified Burkholderiales as well as members of the genera Propionibacterium and Ralstonia were typically the most significant taxa for all atherosclerotic plaques. Other genera such as Burkholderia, Corynebacterium and Sediminibacterium as well as unclassified Comamonadaceae, Oxalobacteraceae, Rhodospirillaceae, Bradyrhizobiaceae and Burkholderiaceae were always found but at low relative abundances of the total 16S rRNA gene population derived from all samples. Also, we found that some bacteria found in plaque samples correlated with some clinical parameters, including total cholesterol, alanine aminotransferase and fibrinogen levels. Finally, our study indicates that some bacterial agents at least partially may be involved in affecting the development of cardiovascular disease through different mechanisms.

Microbiome composition comparison in oral and atherosclerotic plaque from patients with and without periodontitis

“…In both groups, the major bacteria of the network were Agrobacterium sp., Delftia sp., and Rhizobium sp. This echoed a previous report [26]. However, the network around Cutibacterium acnes was different between control and periodontitis samples (Fig.…”

“…The co-occurrence analysis of the microbiome in AP revealed the most significant bacteria were Agrobacterium sp., Delftia sp., and Rhizobium sp., which constituted the network in both groups. Although these are soil bacteria, they were also previously detected in AP [26]. Another significant bacterium, C. acnes, configured the network in the control group.…”

“…The obtained sequence was compared to the database using the CLC Genomics Workbench (CLC bio, Aarhus, Denmark). Principal component analysis (PCA) and clustering analysis were performed using R statistical software [26]. We also performed co-occurrence analysis for the 13 highly detected operational taxonomic units from control and periodontitis samples using the Quantitative Insights Into Microbial Ecology approach [27].…”

Salivary microbiome of an urban Indian cohort and patterns linked to subclinical inflammation

“…In a skin microbiome study, preexisting Paracoccus and Micrococcus associated with susceptibility to inflammation and our findings appear as commensurate (Van Rensburg et al., ). Unclassified Bradyrhizobiaceae have been detected in atherosclerotic lesions (Ziganshina et al., ). Oral Bradyrhizobiaceae were shown to correlate with pain in inflammatory bowel disease (Fourie et al., ).…”

Staphylococcus pasteuri isolates (Spvs) from human atherosclerotic plaques mediate virulence, intracellular resistance and transendothelial invasion of macrophages: A mechanistic paradigm for microbial pathogenicity in atherosclerosis

“…Both viral and bacterial populations have been shown to be present in individuals with atherosclerosis (4,5). While symptomatic infected carotid artery was concurrent with bacterial endocarditis (6) the association of infections such as periodontitis with atherosclerotic diseases is well documented (7, 8 and 9). The development of atherosclerosis is also known to be influenced by certain gut microbes which include species like Escherichia coli, Enterobacter aerogenes, and members of Klebsiella spp.…”