Bacterial colonization during de novo plaque formation

Ramberg, Per; Sekino, Satoshi; Uzel, Naciye Guzin; Socransky, S. S.; Lindhe, Jan

doi:10.1034/j.1600-051x.2003.00419.x

Cited by 56 publications

(54 citation statements)

References 41 publications

(43 reference statements)

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“…Early colonization of tooth-associated ecosystems is a specific and selective process, and the development of a mature community is influenced by these early interbacterial as well as host-bacterium interactions (28,33,42). In the gastrointestinal tract, it has been shown that early colonizers occupy this spatial niche in large numbers, thereby playing an important role in resisting colonization by pathogenic species.…”

mentioning

confidence: 99%

Tobacco Smoking Affects Bacterial Acquisition and Colonization in Oral Biofilms

et al. 2011

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Recent evidence suggests that smoking affects the composition of the disease-associated subgingival biofilm, yet little is known about its effects during the formation of this biofilm. The present investigation was undertaken to examine the contributions of smoking to the composition and proinflammatory characteristics of the biofilm during de novo plaque formation. Marginal and subgingival plaque and gingival crevicular fluid samples were collected from 15 current smokers and from 15 individuals who had never smoked (nonsmokers) following 1, 2, 4, and 7 days of undisturbed plaque formation. 16S rRNA gene cloning and sequencing were used for bacterial identification, and multiplex bead-based flow cytometry was used to quantify the levels of 27 immune mediators. Smokers demonstrated a highly diverse, relatively unstable initial colonization of both marginal and subgingival biofilms, with lower niche saturation than that seen in nonsmokers. Periodontal pathogens belonging to the genera Fusobacterium, Cardiobacterium, Synergistes, and Selenomonas, as well as respiratory pathogens belonging to the genera Haemophilus and Pseudomonas, colonized the early biofilms of smokers and continued to persist over the observation period, suggesting that smoking favors early acquisition and colonization of pathogens in oral biofilms. Smokers also demonstrated an early proinflammatory response to this colonization, which persisted over 7 days. Further, a positive correlation between proinflammatory cytokine levels and commensal bacteria was observed in smokers but not in nonsmokers. Taken together, the data suggest that smoking influences both the composition of the nascent biofilm and the host response to this colonization.

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mentioning

confidence: 99%

Tobacco Smoking Affects Bacterial Acquisition and Colonization in Oral Biofilms

et al. 2011

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“…Additionally, after 4 days of biofilm growth, a visible dental biofilm layer forms. High percentages of visible supragingival biofilms have been observed clinically within this time frame 25 . Therefore, the four day growth period was chosen to simulate a real clinical situation widely confirmed in previous studies.…”

Section: Discussionmentioning

confidence: 99%

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Antoniazzi

Trojahn

Casarin³

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Comparar a eficácia antimicrobiana de clorexidina 0,12% (CHX) e solução de chá verde (CV) (Camellia sinensis) em biofilmes formados em diferentes tensões de oxigênio em um modelo in situ. Método: Vinte e cinco estudantes de odontologia foram elegíveis. Um dispositivo com espécimes de esmalte (EE) padronizados, voltado para o palato e para vestibular foram inseridos nos voluntários por 7 dias. Durante os primeiros quatro dias nenhum agente foi aplicado. A partir do quinto dia, ambos os agentes foram aplicados nos EEs no grupo teste e nenhum agente foi aplicado no grupo EE controle. Após 7 dias, os fragmentos EEs foram removidos dos dispositivos, sonicados, plaqueados em ágar, e incubado durante 24 h a 37 °C para determinar unidades formadoras de colonias (UFCs). Resultado: CHX teve significativamente melhor eficácia em comparação com CV nos EEs para vestibular (1.330 vs. 2.170 UFC/mL) e EEs voltados para o palatal (2.250 vs. 2.520 UFC/mL). Comparações intragrupos mostraram maior eficácia em EEs vestibulares do que as EEs voltadas para o palato (1.330 vs. 2.250 UFC/mL para CHX e 2170 vs, 2520 UFC/mL para CV) em ambas as soluções. Análise dos controles, mostrou a formação de biofilme significativamente maior em comparação com EEs palatinas e bucais. Conclusão: CHX tem uma eficácia maior do que o chá verde em biofilmes formados por 4 dias. A eficácia dos agentes foi reduzida para os biofilmes em ambiente com baixa tensão de oxigênio. A tensão de oxigênio parece influenciar na eficácia dos agentes testados.Descritores: Camellia sinensis; clorexidina; placa dentária. AbstractObjective: To compare the antimicrobial efficacy of a 0.12% chlorhexidine (CHX) and herbal green tea (Camellia sinensis) solution on established biofilms formed at different oxygen tensions in an in situ model. Method: Twenty-five dental students were eligible for the study. In situ devices with standardized enamel specimens (ES) facing the palatal and buccal sides were inserted in the mouths of volunteers for a 7 day period. No agent was applied during the first four days. From the fifth day onward, both agents were applied to the test ES group and no agent was applied to the control ES group. After 7 days the ES fragments were removed from the devices, sonicated, plated on agar, and incubated for 24 h at 37 °C to determine and quantify the colony forming units (CFUs). Result: CHX had significantly higher efficacy compared to green tea on the buccal (1330 vs. 2170 CFU/µL) and palatal (2250 vs. 2520 CFU/µL) ES. In addition, intragroup comparisons showed significantly higher efficacy in buccal ES over palatal ES (1330 vs. 2250 CFU/µL for CHX and 2170 vs, 2520 CFU/µL for CV) for both solutions. Analysis of the ES controls showed significantly higher biofilm formation in palatal ES compared to buccal ES. Conclusion: CHX has higher efficacy than green tea on 4-day biofilms. The efficacy of both agents was reduced for biofilms grown in a low oxygen tension environment. Therefore, the oxygen tension environment seems to influence the eff...

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“…Çocuklarda dişler sürdükten hemen sonra oral hijyen sağlanmaz ve düzenli fırçalama alışkanlığı kazandırılmazsa, bakteriyel biyofilm tabakası oral çevreyle ilişkili olan diş yüzeyine ve gingival marjinlere yerleşerek gingival inflamasyonu başlatabilir (6). Kanıtlar, subgingival plak içindeki gramnegatif bakterilerin ve anaerobların sayısının artmasıyla periodontal hastalıkların geliştiğini göstermektedir (7). Periodontal hastalıklarla sıkça ilişkilendirilen mikroorganizmalar; Aggregatibacter (Actinobacillus), Porphyromonas gingivalis, Tanerella forsythensis ve Treponema denticola' dır (8).…”

Section: Peri̇odontal Hastaliklarda Eti̇yoloji̇unclassified

Çocuklarda ve Adolesanlarda Periodontal Hastalıklar ve Erişkinliğe Etkisi

Durmuşlar¹,

Akcabaş²

2017

J INT Dent Sci

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Bacterial colonization during de novo plaque formation

Cited by 56 publications

References 41 publications

Tobacco Smoking Affects Bacterial Acquisition and Colonization in Oral Biofilms

Tobacco Smoking Affects Bacterial Acquisition and Colonization in Oral Biofilms

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Çocuklarda ve Adolesanlarda Periodontal Hastalıklar ve Erişkinliğe Etkisi

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