Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: A review

Thatoi, Hrudayanath; Das, Sasmita; Mishra, Jibitesh; Rath, Bhagwat Prasad; Das, Nigamananda

doi:10.1016/j.jenvman.2014.07.014

Cited by 385 publications

(178 citation statements)

References 152 publications

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“…13,14 Bacteria decolorize dye by adsorption or degradation. Dye adsorption can be clearly judged by inspecting the cell mass as those adsorbing dyes will be deeply colored, whereas those causing degradation will remain colorless.…”

Section: Introductionmentioning

confidence: 99%

Removal of a reactive dye and hexavalent chromium by a reusable bacteria attached electrospun nanofibrous web

et al. 2015

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A contaminant resistant Lysinibacillus sp. NOSK was isolated from a soil sample and its Reactive Black 5 (RB5) and Cr(VI) removal efficiencies were investigated as a function of changes in the initial pH values, temperature, static/shaking conditions, reactive dye and Cr(VI) concentrations. In this study, an electrospun polysulfone nanofibrous web (PSU-NFW) was found to be effective in attachment of bacterial cells. Bacteria attached PSU-NFWs (bacteria/PSU-NFW) have shown highly efficient removal of RB5, as 99.7 AE 0.9% and 35.8 AE 0.4% for the pristine PSU-NFW. Moreover, the highest Cr(VI) removal efficiencies measured were 98.2 AE 0.6% for bacteria attached PSU-NFW and 32.6 AE 0.6% for the pristine PSU-NFW. Simultaneous removal of RB5 and Cr(VI) were also investigated. Reusability test results indicate that, bacteria/PSU-NFW can be reused for at least 7 cycles with 28.1 AE 0.6% and 66.7 AE 0.8% removal efficiencies for RB5 and Cr(VI), respectively.

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Section: Introductionmentioning

confidence: 99%

Removal of a reactive dye and hexavalent chromium by a reusable bacteria attached electrospun nanofibrous web

et al. 2015

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“…In order to validate that the engineered bacteria still contain the ChrT gene, PCR was used to amplify the ChrT sequence. Genomic DNA of the engineered bacteria was used as a template with the primers (12). It was shown by agarose gel electrophoresis that the length of the PCR amplified product was ~600 bp, which was approximately the same size as the sequence of chromium reductase ChrT (567 bp), as shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

“…To date, various bacteria that are capable of reducing Cr (VI) have been isolated, including Arthrobacter (7), Bacillus (8), Microbacterium (9), Brucella (10) and Pseudomonas (11). In fact, the majority of studies on microorganisms are focused on isolating and culturing wild strains from environments contaminated with chromium (12), while few studies have focused on the associated genes and proteins to reduce chromium (13).…”

Section: Introductionmentioning

confidence: 99%

Reducing capacity and enzyme activity of chromate reductase in a ChrT-engineered strain

Zhou

Dong

Deng³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In order to remediate the metal-contaminated soil and water ecosystems with microorganisms, an engineered strain, which contained the chromate reductase ChrT gene from Serratia sp. S2, was studied in detail for its Cr (VI) reduction efficiency, optimal culture condition and chromate reductase activity. Results demonstrated that the engineered strain had a high Cr (VI) reduction rate of up to 40% at a concentration of 50 mg/l after being cultured for 48 h. Additionally, the optimal culture conditions were pH 7.0 and 37˚C. Furthermore, the carbon sources and metal cations exhibited significant effects on the Cr (VI) reduction rate of the engineered bacterium. Sodium lactate, sodium acetate, Cu 2+ , Co 2+ and Pb 2+ were positively correlated with the reduction rate. Chromate reductase was soluble and presented in the cytoplasm. Furthermore, the enzymatic activity with nicotinamide adenine dinucleotide phosphate, which was as an electron donor, reached 14.83 U/mg.

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“…Several bacteria of the Pseudomonas, Bacillus, Enterobacter, Deinococcus, Shewanella, Agrobacter, Escherichia, Thermus genera are resistant to Cr(VI) and can be used for Cr(VI) remediation [17, [92][93][94][95]. Many mechanisms for this have been proposed, such as generation of Cr(V) by bacterial enzymes mediating transfer of an electron to Cr(VI) and reduction of Cr(VI) to Cr(III) by bacterial chromate reductase [93]. However, bacteria in the form of planktonic cells are ineffective in Cr(VI) remediation as high concentrations of Cr(VI) can kill the cells.…”

Section: Conventional Processes For Chromium Remediationmentioning

confidence: 99%

Removal of chromium from industrial effluents using nanotechnology: a review

Mitra

Sarkar

Sen

2017

Nanotechnol. Environ. Eng.

121

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The presence of chromium in industrial effluents has become a huge problem worldwide as hexavalent chromium is highly toxic to animals due to its ability to generate reactive oxygen species in cells. The trivalent state of chromium, on the other hand, is significantly less toxic and also serves as an essential element in trace amounts. When industries such as electroplating, tannery, dyeing and others release their effluents into water bodies, hexavalent chromium enters the food chain and, consequently, reaches humans in a biomagnified form. Many remediation processes for removal of hexavalent chromium have been researched and reviewed extensively. These include chemical reduction to trivalent chromium, solvent extraction, chelation and adsorption, among others. It has been generally concluded that adsorption (and/or subsequent reduction) of hexavalent chromium is the best method. However, relatively little is known about the potential of using nanoparticles as adsorbents for the removal of hexavalent chromium from industrial effluents. This method of nanoremediation is more effective than conventional remediation methods and is cost-effective for the industry in the long run. This article reviews the various remediation methods of hexavalent chromium, with emphasis on the field of nanoremediation.

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Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: A review

Cited by 385 publications

References 152 publications

Removal of a reactive dye and hexavalent chromium by a reusable bacteria attached electrospun nanofibrous web

Removal of a reactive dye and hexavalent chromium by a reusable bacteria attached electrospun nanofibrous web

Reducing capacity and enzyme activity of chromate reductase in a ChrT-engineered strain

Removal of chromium from industrial effluents using nanotechnology: a review

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