“…A. xylinum was grown on FPY consisting of 2z fructose, 1z Polypepton (Nihon Seiyaku Co., Ltd., Tokyo, Japan), 0.5z yeast extract (Oriental Yeast Co., Ltd., Tokyo), 0.25z K2HPO4 (pH 5.0), or else on a medium of corn steep liquor (Nihon Starch Industry, Kagoshima, Japan) and fructose (CSL-Fru), the composition of which was described elsewhere, 18) at 309 C. E. coli DH5a was grown on Luria-Bertani medium consisting of 1z Tryptone peptone (Becton Dickinson and Company, Sparks, Maryland, USA), 0.5z yeast extract (Becton Dickinson) and 1z NaCl at 379 C. Ampicillin (Sigma Chemical Co., St. Louis, Missouri, USA) was added at 50 ppm for E. coli and at 100 ppm for A. xylinum, and kanamycin (Wako Pure Chemical Industries, Ltd., Osaka, Japan) was added at 100 ppm for E. coli when necessary.…”