Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores

Huang, Li; Du, Xiaoyu; Fan, Suna; Yang, Gesheng; Shao, Huili; Li, Dejian; Cao, Chengbo; Zhu, Yufang; Zhu, Meifang; Zhang, Yaopeng

doi:10.1016/j.carbpol.2019.05.080

Cited by 121 publications

(70 citation statements)

References 63 publications

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“…The data extraction of the included studies is presented in Table 1 . The studies were classified depending on the study design, whereas; twelve studies were in vitro [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], two studies were in vivo [ 31 , 32 ], and four studies conducted both [ 33 , 34 , 35 , 36 ]. The characteristics and outcomes of both in vitro and in vivo studies are presented in Table 2 and Table 3 , respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Overall, the majority of in vitro studies used fibroblastic skin cells. Human dermal fibroblasts (HDFs) were commonly used [ 20 , 21 , 22 , 24 , 27 , 28 , 31 , 34 , 35 , 36 ], followed by T3T mouse fibroblasts [ 19 , 23 , 25 , 29 , 30 ], and L929 mouse fibroblasts [ 33 , 35 ]. However, human epidermal keratinocytes (HEKs) were also used in four studies [ 22 , 31 , 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…Each study included 12–40 animals, but one study [ 34 ] did not disclose the number of animals used. Four studies reported the use of mice [ 31 , 32 , 33 , 34 ], two studies reported the use of rats [ 35 , 36 ], and one study reported the use of porcine [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

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Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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Three-dimensional bioprinting has rapidly paralleled many biomedical applications and assisted in advancing the printing of complex human organs for a better therapeutic practice. The objective of this systematic review is to highlight evidence from the existing studies and evaluate the effectiveness of using natural-based bioinks in skin regeneration and wound healing. A comprehensive search of all relevant original articles was performed based on prespecified eligibility criteria. The search was carried out using PubMed, Web of Science, Scopus, Medline Ovid, and ScienceDirect. Eighteen articles fulfilled the inclusion and exclusion criteria. The animal studies included a total of 151 animals with wound defects. A variety of natural bioinks and skin living cells were implanted in vitro to give insight into the technique through different assessments and findings. Collagen and gelatin hydrogels were most commonly used as bioinks. The follow-up period ranged between one day and six weeks. The majority of animal studies reported that full wound closure was achieved after 2–4 weeks. The results of both in vitro cell culture and in vivo animal studies showed the positive impact of natural bioinks in promoting wound healing. Future research should be focused more on direct the bioprinting of skin wound treatments on animal models to open doors for human clinical trials.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Smandri

Nordin

et al. 2020

Polymers

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“…[91][92][93][94][95][96] A wide variety of cellulose bioinks has been reported in the literature, especially in the last few years. [97][98][99][100][101] The combination of this polysaccharide with alginate yielded a number of different formulations, which were mainly applied for the 3D printing of cartilage scaffolds.…”

Section: Bioink Formulations Based On Alginate and Cellulosementioning

confidence: 99%

Multicomponent polysaccharide alginate-based bioinks

Piras

Smith

2020

J. Mater. Chem. B

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“…Therefore, this issue could be addressed by using TEMPO-mediated oxidation and co-solvents (Dai et al 2019 ). In addition, the incorporation of cellulose nanofibrils into inks provides structural similarity of 3D printed scaffolds to ECMs which possess filamentous architecture, benefitting cell growth and phenotype maintenance in addition to providing tissue with specific properties (Huang et al 2019a ; Prince and Kumacheva 2019 ; Theocharis et al 2016 ). Therefore, artificial ECM-mimetic fibrillar hydrogels are important materials for use in tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

et al. 2020

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A novel biomaterial ink consisting of regenerated silk fibroin (SF) and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose (OBC) nanofibrils was developed for 3D printing lung tissue scaffold. Silk fibroin backbones were cross-linked using horseradish peroxide/H 2 O 2 to form printed hydrogel scaffolds. OBC with a concentration of 7wt% increased the viscosity of inks during the printing process and further improved the shape fidelity of the scaffolds. Rheological measurements and image analyses were performed to evaluate inks printability and print shape fidelity. Three-dimensional construct with ten layers could be printed with ink of 1SF-2OBC (SF/OBC = 1/2, w/w). The composite hydrogel of 1SF-1OBC (SF/OBC = 1/1, w/w) printed at 25 °C exhibited a significantly improved compressive strength of 267 ± 13 kPa and a compressive stiffness of 325 ± 14 kPa at 30% strain, respectively. The optimized printing parameters for 1SF-1OBC were 0.3 bar of printing pressure, 45 mm/s of printing speed and 410 μm of nozzle diameter. Furthermore, OBC nanofibrils could be induced to align along the print lines over 60% degree of orientation, which were analyzed by SEM and X-ray diffraction. The orientation of OBC nanofibrils along print lines provided physical cues for guiding the orientation of lung epithelial stem cells, which maintained the ability to proliferate and kept epithelial phenotype after 7 days’ culture. The 3D printed SF-OBC scaffolds are promising for applications in lung tissue engineering. Electronic supplementary material The online version of this article (10.1007/s10570-020-03526-7) contains supplementary material, which is available to authorized users.

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Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores

Cited by 121 publications

References 63 publications

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Natural 3D-Printed Bioinks for Skin Regeneration and Wound Healing: A Systematic Review

Multicomponent polysaccharide alginate-based bioinks

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

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