Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment

Abstract

The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the chemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or after surgery. This localized chemotherapy would guarantee high drug concentrations at the tumor site while precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs) including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) or neutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxic efficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination of NLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopic breast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-N showed higher encapsulation (97%) and sustained drug release. Both NLCs internalized via endocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50 values below of those of free Dox. Taking advantage of the differential drug release, a mixture of NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo, showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.

Nature‐derived fibrous nanomaterial toward biomedicine and environmental remediation: Today's state and future prospects

“…In this research work, cationic and neutral doxorubicin loaded systems were evaluated in vitro against MDA‐MB‐231 cells followed by an in vivo assay using an orthotopic breast cancer mouse model. Outcomes achieved showed low encapsulation efficiency (48%) and fast release for the cationic drug loaded system while higher encapsulation (97%) and sustained drug release was obtained for the neutral drug loaded system . Ngwabebhoh et al .…”

A review of functionalised bacterial cellulose for targeted biomedical fields

“…27 It may be advantageous to use hybrid biomaterials in which a matrix retains nanoparticles that control the release of the chemotherapeutic drug for the treatment of primary tumour before or after surgical operation. BC hydrogel and nanostructured lipid carriers (NLCs) were assembled by Cacicedo et al (2018). 208 The cationic doxorubicin (Dox) and neutral Dox which used as a drug modal were loaded into NLCs, resulting in the NLC-NH mixture.…”

“…BC hydrogel and nanostructured lipid carriers (NLCs) were assembled by Cacicedo et al (2018). 208 The cationic doxorubicin (Dox) and neutral Dox which used as a drug modal were loaded into NLCs, resulting in the NLC-NH mixture. This study indicated that BC-NLC-NH has a considerable effect on the reduction in tumour growth, prevalence of metastasis, and local drug toxicity.…”

Bacterial Nanocellulose in Dentistry: Perspectives and Challenges

“…The literature describes several in vivo studies in which BC was applied, from the replacement of abdominal skin [23], dorsal implants [24], and brain and breast cancer treatments in rats [25,26], to vascular stents [15,27,28]. Other studies revealing the use of BC as artificial skin, artificial blood vessels and microvessels, wound dressing of secondor third-degree burn ulcers, and dental implants [23] were also reported.…”