Bacterial cellulose/gelatin scaffold loaded with VEGF-silk fibroin nanoparticles for improving angiogenesis in tissue regeneration

Wang, Baoxiu; Lv, Xiangguo; Chen, Shiyan; Li, Zhe; Yao, Jingjing; Peng, Xufeng; Cao, Feng; Xu, Yuhong; Wang, Huaping

doi:10.1007/s10570-017-1472-x

Cited by 31 publications

(16 citation statements)

References 43 publications

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“…Delivery of angiogenesis triggering factors such as VEGF can enhance formation of blood vessels and promote tissue healing process. Various studies have been conducted for delivery of bioactive agents such as growth factors for promoting the neovascularization in vitro and in vivo (Lee et al, 2017; Wang B. et al, 2017). Controlled delivery of VEGF with appropriate release profile plays an important role in vascularization.…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Fathi‐Achachelouei

Knopf‐Marques

Silva

et al. 2019

Front. Bioeng. Biotechnol.

267

146

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Advances in nanoparticle (NP) production and demand for control over nanoscale systems have had significant impact on tissue engineering and regenerative medicine (TERM). NPs with low toxicity, contrasting agent properties, tailorable characteristics, targeted/stimuli-response delivery potential, and precise control over behavior (via external stimuli such as magnetic fields) have made it possible their use for improving engineered tissues and overcoming obstacles in TERM. Functional tissue and organ replacements require a high degree of spatial and temporal control over the biological events and also their real-time monitoring. Presentation and local delivery of bioactive (growth factors, chemokines, inhibitors, cytokines, genes etc.) and contrast agents in a controlled manner are important implements to exert control over and monitor the engineered tissues. This need resulted in utilization of NP based systems in tissue engineering scaffolds for delivery of multiple growth factors, for providing contrast for imaging and also for controlling properties of the scaffolds. Depending on the application, materials, as polymers, metals, ceramics and their different composites can be utilized for production of NPs. In this review, we will cover the use of NP systems in TERM and also provide an outlook for future potential use of such systems.

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Section: Polymeric Nanoparticlesmentioning

confidence: 99%

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Fathi‐Achachelouei

Knopf‐Marques

Silva

et al. 2019

Front. Bioeng. Biotechnol.

267

146

View full text Add to dashboard Cite

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“…BC has fantastic characteristics for tissue engineering applications, yet it lacks the capacity to develop proper vascularization, leading to cellular apoptosis. To attenuate this problem, Wang et al . developed a porous scaffold biocomposite of BC/gelatin, containing SF nanoparticles loaded with vascular endothelial growth factor (VEGF).…”

Section: Applicationsmentioning

confidence: 99%

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Carvalho

Guedes

Sousa

et al. 2019

Biotechnology Journal

122

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Bacterial cellulose (BC) is a nanocellulose form produced by some nonpathogenic bacteria. BC presents unique physical, chemical, and biological properties that make it a very versatile material and has found application in several fields, namely in food industry, cosmetics, and biomedicine. This review overviews the latest state‐of‐the‐art usage of BC on three important areas of the biomedical field, namely delivery systems, wound dressing and healing materials, and tissue engineering for regenerative medicine. BC will be reviewed as a promising biopolymer for the design and development of innovative materials for the mentioned applications. Overall, BC is shown to be an effective and versatile carrier for delivery systems, a safe and multicustomizable patch or graft for wound dressing and healing applications, and a material that can be further tuned to better adjust for each tissue engineering application, by using different methods.

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“…SFNPs have been previously used in the delivery of VEGF. , Mild preparation conditions (self-assembly without any toxic organic reagents or harsh reaction conditions) maintained the activity of the growth factor. In the present study, SFNPs were used as a carrier of heparin to enhance the anticoagulant properties of the surfaces.…”

Section: Resultsmentioning

confidence: 99%

“…SFNPs were prepared by self-assembly of SF molecular chains, in accordance with methods reported previously. , Briefly, a 1 mL solution of silk fibroin solution (2% w/v) was prepared from freeze-dried SF powder, which was then mixed with 250 μL of ethanol. It was gently stirred (100 rpm) at room temperature for 5 min then incubated at −20 °C for 24 h. The mixture was then defrosted at room temperature to obtain an SFNP emulsion.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Tubular bacterial nanocellulose (BNC) has been demonstrated to be a promising natural biomaterial for applications in small-caliber vascular grafts due to its unique characteristics such as the extracellular matrix-like 3D nanofibril network structure, high purity and water retention, good plasticity, and extraordinary mechanical properties in a wet state, combined with excellent biocompatibility. − Both in vitro and in vivo studies have been conducted, the tubular BNC material demonstrating considerable potential. − Because the adhesion and spread of endothelial cells on unmodified BNC are limited, silk fibroin (SF) was introduced to enhance the properties of the luminal wall of the artificial blood vessels and achieve rapid endothelialization.…”

Section: Introductionmentioning

confidence: 99%

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Improved Performance of Bacterial Nanocellulose Conduits by the Introduction of Silk Fibroin Nanoparticles and Heparin for Small-Caliber Vascular Graft Applications

Bao

Hong

et al. 2020

Biomacromolecules

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Bacterial nanocellulose (BNC) is a promising material for small-caliber artificial blood vessels, although promoting its anticoagulant properties with more rapid endothelialization would improve long-term patency. Silk fibroin nanoparticles (SFNP) were introduced into the luminal wall surface of BNC conduits both with and without heparin (Hep) through pressurization followed by fixation. Hep was introduced in two ways: (1) embedded within SF nanoparticles to form SF-HepNPs for construction of the BNC-SF-HepNP conduit and (2) chemically grafted onto BNC and BNC-SFNP to form BNC-Hep and BNC-SFNP-Hep conduits. Fourier transform infrared spectroscopy confirmed the formation of SF-HepNPs, although they did not incorporate into the fibrillar network due to their large size. Hep was successfully grafted onto BNC and BNC-SFNP, verified by toluidine blue staining. The hemocompatibility and cytocompatibility of the five samples (BNC, BNC-SFNP, BNC-SF-HepNP, BNC-Hep, and BNC-SFNP-Hep conduits) were compared in vitro. The heparinized BNC-Hep and BNC-SFNP-Hep conduits improved the anticoagulant properties, and BNC-SFNP-Hep promoted human umbilical vein endothelial cell proliferation but also controlled excessive human arterial smooth muscle cell proliferation, assisting rapid endothelialization and improving lumen patency. No significant inflammatory reaction or material degradation was observed after subcutaneous implantation for 4 weeks. Autogenous tissues were observed around the conduits, and cells infiltrated into the edges of all samples, the BNC-SFNP conduit causing the deepest infiltration, providing an appropriate microenvironment for angiogenesis when used in small-caliber blood vessel applications. Few inflammatory cells were found around the BNC-Hep and BNC-SFNP-Hep conduits. Thus, the anticoagulant properties of the BNC-SFNP-Hep conduit and its stimulation of endothelialization suggest that it has great potential in clinical applications as a small-caliber artificial blood vessel.

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Bacterial cellulose/gelatin scaffold loaded with VEGF-silk fibroin nanoparticles for improving angiogenesis in tissue regeneration

Cited by 31 publications

References 43 publications

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Improved Performance of Bacterial Nanocellulose Conduits by the Introduction of Silk Fibroin Nanoparticles and Heparin for Small-Caliber Vascular Graft Applications

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