Bacterial Cellulose From Rice Waste Water and Its Composite Which Are Deposited Nanoparticle as an Antimicrobial Material

Rohaeti, Eli; Laksono, Endang Widjajanti; Rakhmawati, Anna

doi:10.20961/alchemy.12.1.946.70-87

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…First, the positively charged chitosan interacts with negatively charged bacteria, leading to an increase in permeability of bacterial membrane, which leads to inhibition of test strain cells growth. Another mechanism involves the binding of chitosan to DNA, which can suppress the production of bacterial mRNA [34]. Since Ch is antibacterial, the incorporation of Ch in BC should lead to appearance of antimicrobial properties in the BC/Ch composite film.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials

Savitskaya

Kistaubayeva

Digel

et al. 2017

Eurasian Chem. Tech. J.

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New bacterial cellulose/chitosan (BC/Ch) nanocomposite films were obtained using a simple procedure by immersing BC synthesized by Komagataeibacter xylinus in 1% acetic acid solutions of Ch with the degree of deacetylation 75-85% of medium molecular weight. The BC and BC/Ch composites chemical composition was examined by FTIR, the mechanical properties by a tensile tester, surface morphology by scanning electron microscopy, and antibacterial activity against S. aureus, E. coli and P. aeruginosa by diffusion and joint incubation methods. The FTIR spectra indicated the intermolecular interaction between BC and Ch. Due to addition of 0.6% (w/v) Ch, the films of BC/Ch become more homogeneous with a significantly denser fibril structure, smaller pore diameter and higher surface area in comparison to those of pure BC films. Micro-(15-35 nm) and macrofibrils (50-150 nm) in both BC and BC/Ch films are joined in ribbon-like fibers, providing a high degree of mechanical strength (Young's modulus: 33-36 MPa, tensile strength and elongation et break: 17, 22 MPa). The obtained hybrid material is transparent, flexible and displays good water absorption capacity and water vapor permeability. The films have reasonable thermal stability to be in contact with body or during steam sterilization, since maximum degradation temperature (Td) of both biocomposites is around 400-600 °C. The disc diffusion method confirmed that the BC/Ch films have predominantly non-diffusible antibacterial properties. Antibacterial assessment by the joint incubation method proved that addition of Ch to BC films resulted in significant growth inhibition against target bacteria. The BC/Ch biocomposites' notable properties make them suitable for wound healing applications.

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Section: Resultsmentioning

confidence: 99%

Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials

Savitskaya

Kistaubayeva

Digel

et al. 2017

Eurasian Chem. Tech. J.

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show abstract

“…First, positively charged chitosan interacts with negatively charged bacteria, leading to an increase in permeability of bacterial membrane, which leads to inhibition of cell growth in test strains. Another mechanism involves binding of chitosan to DNA, which can suppress the production of bacterial mRNA [11].…”

Section: Fig2sorption Dynamics Of Protein Exometabolites On the Bc/ch Filmmentioning

confidence: 99%

“…It should be noted that antibacterial activity of B. subtilis postbiotic is enhanced by the action of lytic enzymes that actively lyse cells of both gram-positive and gram-negative microorganisms. Interestingly, when analyzing the microbial contamination of wounds of various etiology, it was found that the presence of B. subtilis exometabolites in them prevents the development of purulent inflammation [11]. The above data are part of the rationale for the correct choice of these components for immobilization on BC for wound dressing application.…”

Section: Fig2sorption Dynamics Of Protein Exometabolites On the Bc/ch Filmmentioning

confidence: 99%

Wound-healing activity of immobilized postbiotics from Bacillus subtilis exometabolites

2021

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The biological activity of postbiotic from Bacillus subtilis exometabolites was determined due to protein content - 0.541±13.4 mg/ml, the level of proteases - 7.8±0.3 U/ml and the presence of antimicrobial substances. A biocomposite material was developed by co-aggregation of bacterial cellulose, chitosan and Bacillus exometabolites. Modified BC gel film possesses high antagonistic activity against causative agents of wound infections: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis. The use of the obtained material in treatment of wounds on laboratory animals reduces healing time by an average of 20%. The developed bioactive wound dressings is intended for local application in order to optimize the wound healing process.

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Impact of exosome‐loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

Nooshabadi

Khanmohamadi

Valipour

et al. 2020

J Biomedical Materials Res

101

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Combat or burn injuries are associated with a series of risks, such as microbial infection, an elevated level of inflammatory response, and pathologic scar tissue formation, which significantly postpone wound healing and also lead to impaired repair. Skin engineering for wound healing requires a biomimetic dressing substrate with ideal hydrophilicity, holding antioxidant and antimicrobial properties. In addition, available bioactive specification is required to reduce scar formation, stimulate angiogenesis, and improve wound repair. In this study, we successfully fabricated chitosan (Ch)–based hydrogel enriched with isolated exosome (EXO) from easy‐accessible stem cells, which could promote fibroblast cell migration and proliferation in vitro. Full‐thickness excisional wound model was used to investigate the in vivo dermal substitution ability of the fabricated hydrogel composed Ch and EXO substrates. Our finding confirmed that the wounds covered with Ch scaffold containing isolated EXO have nearly 83.6% wound closure ability with a high degree of re‐epithelialization, whereas sterile gauze showed 51.5% of reduction in wound size. In summary, obtained results imply that Ch‐glycerol‐EXO hydrogel construct can be utilized at the full‐thickness skin wound substitution and skin tissue engineering.

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Bacterial Cellulose From Rice Waste Water and Its Composite Which Are Deposited Nanoparticle as an Antimicrobial Material

Cited by 7 publications

References 15 publications

Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials

Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials

Wound-healing activity of immobilized postbiotics from Bacillus subtilis exometabolites

Impact of exosome‐loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

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