Bacterial cell recovery after hollow fiber microfiltration sample concentration: Most probable bacterial composition in frozen vegetables

Irwin, Peter L.; He, Yiping; Nguyen, Ly; Gehring, Matthew; Gehring, Andrew G.; Chen, Chin-Yi; Capobianco, Joseph A.

doi:10.1016/j.lwt.2020.110647

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Song et al studied the bacterial adhesion and growth behaviors on a membrane surface, showing that biofouling reduced the interaction energy between the membrane and, most importantly, the antifouling capability decreased after chemical cleaning . The in-pore clogged microparticles are unlikely to be collected after the microfiltration process. ,– Standard techniques to clear membrane fouling and prevent microparticle clogging include backwashing , and acoustic vibration. ,, For instance, backwashing was applied to release coagulated colloidal particles in the feedwater for real-time monitoring using a novel online light scattering technology . Also, optimal backwashing was experimentally explored to solve the membrane fouling problem in the combined water treatment process of alumina microfiltration and pure polypropylene beads .…”

Section: Introductionmentioning

confidence: 99%

Dean Vortex Trapping for Concentrating Escherichia coli in a Cross-Flow Microfilter

Zhang,

Zhao,

et al. 2023

ACS Appl. Eng. Mater.

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Membrane-based microfiltration is a gold standard widely used in clinical applications, water treatment, or water safety monitoring for sample processing to purify or concentrate targeted microparticles in a sample liquid. In the concentration of targeted microparticles, membrane fouling is one of the main problems contributing to a low recovery rate. Research efforts to improve the recovery rate focus on membrane materials, chemical treatment, or physical techniques. Periodic backwashing and acoustic vibration are the most used physical techniques to release clogged microparticles on the membrane surface. However, they are less effective for low concentrations of microparticles. In addition, highintensity acoustic vibration and high-pressure backwashing are incompatible with bioparticle processing, such as Escherichia coli (E. coli) bacteria. Herein, we proposed a Dean vortex-assisted cross-flow microfilter with a spiral-shaped microchannel to improve the recovery rate of the E. coli concentration in water samples. The Dean vortex formed in the microchannel efficiently prevented the clogging of E. coli on the membrane surface, demonstrating a significantly improved recovery rate from ∼40 to >90%. Moreover, we applied the Dean vortex-assisted cross-flow microfilter for concentrating E. coli spiked in tap water samples, proving its potential for real-life applications in processing low-concentration bacterial samples in environmental and water safety monitoring.

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