Bacterial cell membranes and their role in daptomycin resistance: A review

Nguyen, April H.; Hood, Kara S.; Mileykovskaya, Eugenia; Miller, William R.; Tran, Truc T.

doi:10.3389/fmolb.2022.1035574

Cited by 17 publications

(8 citation statements)

References 90 publications

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“…The daptomycin mechanism consists of membrane permeabilization, the inhibition of cell wall synthesis, and the alternation of membrane fluidity and curvature. Unfortunately, its extensive clinical use has already led to resistance due to alterations in the membrane lipid composition such as a significant decrease in the amount of phosphatidylglycerol (PG) or an increase in cardiolipin (CL), which limits daptomycin’s action against bacteria [ 99 ].…”

Section: Amp Lipidationmentioning

confidence: 99%

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

et al. 2023

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Antimicrobial peptides (AMPs) have recently gained attention as a viable solution for combatting antibiotic resistance due to their numerous advantages, including their broad-spectrum activity, low propensity for inducing resistance, and low cytotoxicity. Unfortunately, their clinical application is limited due to their short half-life and susceptibility to proteolytic cleavage by serum proteases. Indeed, several chemical strategies, such as peptide cyclization, N-methylation, PEGylation, glycosylation, and lipidation, are widely used for overcoming these issues. This review describes how lipidation and glycosylation are commonly used to increase AMPs’ efficacy and engineer novel AMP-based delivery systems. The glycosylation of AMPs, which involves the conjugation of sugar moieties such as glucose and N-acetyl galactosamine, modulates their pharmacokinetic and pharmacodynamic properties, improves their antimicrobial activity, and reduces their interaction with mammalian cells, thereby increasing selectivity toward bacterial membranes. In the same way, lipidation of AMPs, which involves the covalent addition of fatty acids, has a significant impact on their therapeutic index by influencing their physicochemical properties and interaction with bacterial and mammalian membranes. This review highlights the possibility of using glycosylation and lipidation strategies to increase the efficacy and activity of conventional AMPs.

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Section: Amp Lipidationmentioning

confidence: 99%

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

et al. 2023

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“…We observed that daptomycin bound evenly to the outside (mother) vesicle, however the vesicles in the lumen did not show fluorescence in the daptomycin channel, and presumably remained completely unaffected by its activity. According to recent molecular dynamics simulations, upon contact with Ca 2+ ions daptomycin forms tetramers, which can reversibly flip between the outer and inner leaflets of the membrane [102]. We cannot unambiguously conclude whether daptomycin embeds only in the outer leaflet or integrates also in the inner monolayer of the membrane.…”

Section: Resultsmentioning

confidence: 89%

Tunable biomimetic bacterial membranes from binary and ternary lipid mixtures and their application in antimicrobial testing

Krok

Stephan

Dimova

et al. 2023

Preprint

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Reconstruction of accurate yet simplified mimetic models of cell membranes is a very challenging goal of synthetic biology. To date, most of the research focuses on the development of eukaryotic cell membranes, while reconstitution of their prokaryotic counterparts has not been fully addressed, and the proposed models do not reflect well the complexity of bacterial cell envelopes. Here, we describe the reconstitution of biomimetic bacterial membranes with an increasing level of complexity, developed from binary and ternary lipid mixtures. Giant unilamellar vesicles composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); PC and phosphatidylglycerol (PG); PE and PG; PE, PG and cardiolipin (CA) at varying molar ratios were successfully prepared by the electroformation method. Each of the proposed mimetic models focuses on reproducing specific membrane features such as membrane charge, curvature, leaflets asymmetry, or the presence of phase separation. GUVs were characterized in terms of size distribution, surface charge, and lateral organization. Finally, the developed models were tested against the lipopeptide antibiotic daptomycin. The obtained results showed a clear dependency of daptomycin binding efficiency on the amount of negatively charged lipid species present in the membrane. We anticipate that the models proposed here can be applied not only in antimicrobial testing but also serve as platforms for studying fundamental biological processes in bacteria as well as their interaction with physiologically relevant biomolecules.

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“…Acute bacterial infection is the most common type of infection in clinical practice. Peritonitis, liver abscess, and other diseases caused by P. aeruginosa are also very common in hospitals (Nguyen et al 2022). In the present study, we designed an acute mouse abdominal infection model caused by P. aeruginosa to evaluate the in vivo e cacy of berberine hydrochloride.…”

Section: Disscussionmentioning

confidence: 99%

Mechanisms of action of berberine hydrochloride in planktonic cells and biofilms of Pseudomonas aeruginosa

Liu,

Tang,

Jiang

et al. 2023

Preprint

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The growing prevalence of extensive drug-resistant and pan-drug resistant Pseudomonas aeruginosa (P. aeruginosa) has become a significant concern for global public health, particularly due to the formation of biofilms. Consequently, it is crucial to explore the development of novel antimicrobials that specifically target P. aeruginosa and its biofilms. Here we found a natural compound named berberine hydrochloride, subsequently, Crystalline violet staining and Confocal laser scanning microscope further demostrated that berberine hydrochloride suppress biofilm. Additionally, it was found that the expression of pslA and other biofilm-related genes were downregulated. Compared with available antibiotics, berberine hydrochloride has multiple modes of action in P. aeruginosa. The antimicrobial mechanism showed that berberine hydrochloride might have antimicrobial effects by damaging bacterial cell membranes, generating reactive oxygen species (ROS) and lowering intracellular adenosine triphosphate (ATP). Futhermore, berberine hydrochloride possesses various attributes, including minimal cytotoxicity and reduced susceptibility to drug resistance. In a mice peritonitis model, it was reported to inhibit the growth of P. aeruginosa significantly and displayed high bacteriostatic action. Collectively, the berberine hydrochloride is a safe and effective antibacterial agent for inhibiting P. aeruginosa.

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Bacterial cell membranes and their role in daptomycin resistance: A review

Cited by 17 publications

References 90 publications

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

Glycosylation and Lipidation Strategies: Approaches for Improving Antimicrobial Peptide Efficacy

Tunable biomimetic bacterial membranes from binary and ternary lipid mixtures and their application in antimicrobial testing

Mechanisms of action of berberine hydrochloride in planktonic cells and biofilms of Pseudomonas aeruginosa

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