Bacterial bug-out bags: outer membrane vesicles and their proteins and functions

Dineshkumar, Kesavan; Aparna, V. Sudhakaran; Wu, Liang; Wan, Jie; Abdelaziz, Mohamed Hamed; Su, Zhaoliang; Wang, Shengjun; Xia, Huan

doi:10.1007/s12275-020-0026-3

Cited by 12 publications

(9 citation statements)

References 143 publications

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“…These proteins have been shown to be highly involved in host interactions by facilitating mucus- and epithelial cell- binding functions extracellularly (MoonProt 4 ). Similarly, moonlighting proteins have previously been shown to be in high abundance on outer membrane vesicles from Gram-negative bacteria ( Dineshkumar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 88%

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Extracellular membrane vesicles from Limosilactobacillus reuteri strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1

et al. 2022

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Bacterial extracellular membrane vesicles (MV) are potent mediators of microbe-host signals, and they are not only important in host-pathogen interactions but also for the interactions between mutualistic bacteria and their hosts. Studies of MV derived from probiotics could enhance the understanding of these universal signal entities, and here we have studied MV derived from Limosilactobacillus reuteri DSM 17938 and BG-R46. The production of MV increased with cultivation time and after oxygen stress. Mass spectrometry-based proteomics analyses revealed that the MV carried a large number of bacterial cell surface proteins, several predicted to be involved in host-bacteria interactions. A 5′-nucleotidase, which catalyze the conversion of AMP into the signal molecule adenosine, was one of these and analysis of enzymatic activity showed that L. reuteri BG-R46 derived MV exhibited the highest activity. We also detected the TLR2 activator lipoteichoic acid on the MV. In models for host interactions, we first observed that L. reuteri MV were internalized by Caco-2/HT29-MTX epithelial cells, and in a dose-dependent manner decreased the leakage caused by enterotoxigenic Escherichia coli by up to 65%. Furthermore, the MV upregulated IL-1β and IL-6 from peripheral blood mononuclear cells (PBMC), but also dampened IFN-γ and TNF-α responses in PBMC challenged with Staphylococcus aureus. Finally, we showed that MV from the L. reuteri strains have an antagonistic effect on the pain receptor transient receptor potential vanilloid 1 in a model with primary dorsal root ganglion cells from rats. In summary, we have shown that these mobile nanometer scale MV reproduce several biological effects of L. reuteri cells and that the production parameters and selection of strain have an impact on the activity of the MV. This could potentially provide key information for development of innovative and more efficient probiotic products.

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Section: Discussionmentioning

confidence: 88%

“…Similarly, moonlighting proteins have previously been shown to be in high abundance on outer membrane vesicles from Gramnegative bacteria (Dineshkumar et al, 2020). Already in 1967 it was observed that extracellular products from pathogen bacteria could be engaged in immunomodulatory activities (Chatterjee and Das, 1967).…”

mentioning

confidence: 97%

Extracellular membrane vesicles from Limosilactobacillus reuteri strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1

et al. 2022

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“…For example, the cytoplasmic chaperon proteins GroEL and DnaK, which were primarily known to play a role in protein folding, are relocated to the cell surface or are secreted during invasion ( 48 ). Another very intriguing prototypical moonlighting protein is enolase, which is often found in vesicles produced by both Gram-positive and Gram-negative bacteria; indeed, it can act as bacterial adhesin binding host proteins, and plays a role in infections ( 25 , 49 – 51 ). Hence, the presence of Eno1 on the surface of S. coelicolor MVs, as demonstrated by PK treatment, might suggest a possible role of this protein in interacting with target cells.…”

Section: Discussionmentioning

confidence: 99%

Streptomyces coelicolor Vesicles: Many Molecules To Be Delivered

Faddetta

Renzone

Vassallo

et al. 2022

Appl Environ Microbiol

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Streptomycetes are widely distributed in nature and characterized by a complex life cycle that involves morphological differentiation. They are very relevant in industry because they produce about half of all clinically used antibiotics, as well as other important pharmaceutical products of natural origin. Streptomyces coelicolor is a model organism for the study of bacterial differentiation and bioactive molecule production. S. coelicolor produces extracellular vesicles which carry many molecules, such as proteins and metabolites, including antibiotics.

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“…More than a third of the bacterial proteome is likely to be extra-cytoplasmic [ 90 ], located in membranes in the periplasmic space, or secreted. Different models have been proposed for bacterial exudation especially through outer-membrane vesiculation [ 91 , 92 , 93 , 94 ]. Production of outer-membrane vesicles (OMVs) underlies evolutionarily conserved mechanisms important in cell communication.…”

Section: Discussionmentioning

confidence: 99%

Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition

Zhour

Bray

Dandache

et al. 2022

IJMS

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Diazotrophic bacteria isolated from the rhizosphere of a wild wheat ancestor, grown from its refuge area in the Fertile Crescent, were found to be efficient Plant Growth-Promoting Rhizobacteria (PGPR), upon interaction with an elite wheat cultivar. In nitrogen-starved plants, they increased the amount of nitrogen in the seed crop (per plant) by about twofold. A bacterial growth medium was developed to investigate the effects of bacterial exudates on root development in the elite cultivar, and to analyze the exo-metabolomes and exo-proteomes. Altered root development was observed, with distinct responses depending on the strain, for instance, with respect to root hair development. A first conclusion from these results is that the ability of wheat to establish effective beneficial interactions with PGPRs does not appear to have undergone systematic deep reprogramming during domestication. Exo-metabolome analysis revealed a complex set of secondary metabolites, including nutrient ion chelators, cyclopeptides that could act as phytohormone mimetics, and quorum sensing molecules having inter-kingdom signaling properties. The exo-proteome-comprised strain-specific enzymes, and structural proteins belonging to outer-membrane vesicles, are likely to sequester metabolites in their lumen. Thus, the methodological processes we have developed to collect and analyze bacterial exudates have revealed that PGPRs constitutively exude a highly complex set of metabolites; this is likely to allow numerous mechanisms to simultaneously contribute to plant growth promotion, and thereby to also broaden the spectra of plant genotypes (species and accessions/cultivars) with which beneficial interactions can occur.

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Bacterial bug-out bags: outer membrane vesicles and their proteins and functions

Cited by 12 publications

References 143 publications

Extracellular membrane vesicles from Limosilactobacillus reuteri strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1

Extracellular membrane vesicles from Limosilactobacillus reuteri strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1

Streptomyces coelicolor Vesicles: Many Molecules To Be Delivered

Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition

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