Bacterial Biofilms in Nature and Disease

Costerton, J. W.; Cheng, K.-J.; Geesey, Gill G.; Ladd, T I; Nickel, J. Curtis; Dasgupta, Mrinal; Marrie, Thomas J.

doi:10.1146/annurev.mi.41.100187.002251

Cited by 2,773 publications

(1,186 citation statements)

References 97 publications

(116 reference statements)

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“…The Gram-negative bacterium P. aeruginosa produces prototypic PAMPs including LPS; however, it also possesses the ability to establish a persistent state of infection, especially in the lungs of patients suffering from cystic fibrosis and other chronic obstructive pulmonary diseases (42,43). The establishment of chronic P. aeruginosa infections correlates with the formation of biofilms (20,44), which are polymeric matrices of bacterial growth (45). In turn, biofilm formation and maintenance is a complex process regulated by C12 (20,21,44).…”

Section: Discussionmentioning

confidence: 99%

N-(3-Oxo-acyl)homoserine Lactones Signal Cell Activation through a Mechanism distinct from the Canonical Pathogen-associated Molecular Pattern Recognition Receptor Pathways

Kravchenko

Kaufmann

Mathison

et al. 2006

Journal of Biological Chemistry

120

163

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Innate immune system receptors function as sensors of infection and trigger the immune responses through ligand-specific signaling pathways. These ligands are pathogen-associated products, such as components of bacterial walls and viral nuclear acids. A common response to such ligands is the activation of mitogen-activated protein kinase p38, whereas doublestranded viral RNA additionally induces the phosphorylation of eukaryotic translation initiation factor 2␣ (eIF2␣). Here we have shown that p38 and eIF2␣ phosphorylation represent two biochemical markers of the effects induced by N-(3-oxo-acyl)homoserine lactones, the secreted products of a number of Gramnegative bacteria, including the human opportunistic pathogen Pseudomonas aeruginosa. Furthermore, N-(3-oxo-dodecanoyl)homoserine lactone induced distension of mitochondria and the endoplasmic reticulum as well as c-jun gene transcription. These effects occurred in a wide variety of cell types including alveolar macrophages and bronchial epithelial cells, requiring the structural integrity of the lactone ring motif and its natural stereochemistry. These findings suggest that N-(3-oxo-acyl)homoserine lactones might be recognized by receptors of the innate immune system. However, we provide evidence that N-(3-oxo-dodecanoyl)homoserine lactone-mediated signaling does not require the presence of the canonical innate immune system receptors, Toll-like receptors, or two members of the NLR/Nod/Caterpillar family, Nod1 and Nod2. These data offer a new understanding of the effects of N-(3-oxo-dodecanoyl)homoserine lactone on host cells and its role in persistent airway infections caused by P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

N-(3-Oxo-acyl)homoserine Lactones Signal Cell Activation through a Mechanism distinct from the Canonical Pathogen-associated Molecular Pattern Recognition Receptor Pathways

Kravchenko

Kaufmann

Mathison

et al. 2006

Journal of Biological Chemistry

120

163

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“…Many S. aureus infections are thought to involve the formation of biofilm, surface-associated communities of microbes encompassed by an extracellular matrix (1,2). Several S. aureus genes have been reported to contribute to its biofilm-forming ability, including agr (3)(4)(5), sarA (5-7), sigB (8), ica (9), rbf (10), tcaR (11), arlRS (12,13), and alsSD (14).…”

Section: O Ne Of the Primary Causative Agents Of Nosocomial Infectionmentioning

confidence: 99%

The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus

Rice

Mann

Endres

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

589

654

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The Staphylococcus aureus cidA and lrgA genes have been shown to affect cell lysis under a variety of conditions during planktonic growth. It is hypothesized that these genes encode holins and antiholins, respectively, and may serve as molecular control elements of bacterial cell lysis. To examine the biological role of cell death and lysis, we studied the impact of the cidA mutation on biofilm development. Interestingly, this mutation had a dramatic impact on biofilm morphology and adherence. The cidA mutant (KB1050) biofilm exhibited a rougher appearance compared with the parental strain (UAMS-1) and was less adherent. Propidium iodide staining revealed that KB1050 accumulated more dead cells within the biofilm population relative to UAMS-1, indicative of reduced cell lysis. In agreement with this finding, quantitative real-time PCR experiments demonstrated the presence of 5-fold less genomic DNA in the KB1050 biofilm relative to UAMS-1. Furthermore, treatment of the UAMS-1 biofilm with DNase I caused extensive cell detachment, whereas similar treatment of the KB1050 biofilm had only a modest effect. These results demonstrate that cidA-controlled cell lysis plays a significant role during biofilm development and that released genomic DNA is an important structural component of S. aureus biofilm.autolysis ͉ extracellular DNA ͉ programmed cell death ͉ holin

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“…Particles (both passive and active) are often immersed in fluids that are non-Newtonian (e.g., polymer-filled materials [4,14], spermatozoa swimming through the viscoelastic cervical mucus [15], bacteria in cross-linked polymer gels [16]). The complexity of the suspending liquid strongly alters the observed particle dynamics with respect to that with a Newtonian fluid in analogous flow conditions.…”

Section: Gadavino@uninaitmentioning

confidence: 99%

Bistability and metabistability scenario in the dynamics of an ellipsoidal particle in a sheared viscoelastic fluid

et al. 2014

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The motion of an ellipsoidal particle in a viscoelastic liquid subjected to an unconfined shear flow is addressed by numerical simulations. A complex dynamics is found with different transients and long-time regimes depending on the Deborah number De (De is the product of the viscoelastic liquid intrinsic time times the applied shear rate). Spiraling orbits toward a log-rolling motion around the vorticity are observed for low Deborah numbers, whereas the particle aligns with its major axis near to the flow direction at high Deborah numbers. The transition from vorticity to flow alignment is characterized by a periodic regime with small amplitude oscillations around orientations progressively shifting from vorticity to flow direction by increasing De. A range of Deborah numbers is detected such that the periodic solution coexists with the flow alignment regime (bistability). A further range of De is found where flow alignment is attained differently for particles starting far from or next to the shear plane: in the latter case, very long transients are found; hence an effective bistability (metabistability) is predicted to occur in a large time lapse before reaching the fully aligned state. Finally, the computed Deborah number values for flow alignment favorably compare with available experimental data. © 2014 American Physical Society

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Bacterial Biofilms in Nature and Disease

Cited by 2,773 publications

References 97 publications

N-(3-Oxo-acyl)homoserine Lactones Signal Cell Activation through a Mechanism distinct from the Canonical Pathogen-associated Molecular Pattern Recognition Receptor Pathways

N-(3-Oxo-acyl)homoserine Lactones Signal Cell Activation through a Mechanism distinct from the Canonical Pathogen-associated Molecular Pattern Recognition Receptor Pathways

The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus

Bistability and metabistability scenario in the dynamics of an ellipsoidal particle in a sheared viscoelastic fluid

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