Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies

Fuente-Núñez, César de la; Reffuveille, Fany; Fernández, Lucía; Hancock, Robert E. W.

doi:10.1016/j.mib.2013.06.013

Cited by 648 publications

(472 citation statements)

References 64 publications

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“…However, host immune responses are not much effective against bacterial biofilms in comparison with their planktonic counterpart (Schultz et al, 2010). Many bacterial pathogens that are initially considered as strictly extracellular can persist inside the host by the formation of biofilm through the process of adaptation (De la Fuente-Nunez et al, 2013) that results in the evasion of the bacteria from innate immunity of the host. The evasion of biofilms from host innate response proves harmful to the host, as the inflammatory influx released by the body in response to the bacterial infection may damage the host tissues (Archer et al, 2011).…”

Section: Biofilm and Drug Resistancementioning

confidence: 99%

Review on biofilm formation and its control options

2017

Int. J. Adv. Res. Biol. Sci

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The generalized idea that bacteria have a unicellular way of life is not entirely accurate, given that pure planktonic growth is uncommon. Biofilms are defined as an organized group of microorganisms living within a self-produced matrix of polymeric substances which gets attached to several surfaces. This review was done with the objective to give an overview on the mechanism of biofilm formation and highlight its veterinary and public health implications and control options. The formation of a biofilm occurs in five stages, stage of cell attachment, stage securely affixing of cells, stage of micro-colonies formation and beginning to mature, Stage of more maturation, and stage of dispersal of cells from the biofilm. Factors controlling cell attachment include nature of surface, properties of medium and properties of the microbial cell surface. Formation of a biofilm is dependent on the interaction between the environmental stimuli and the reciprocation of the corresponding signalling events by the microorganisms. Biofilms are composed primarily of microbial cells and EPS. The importance of biofilm in disease processes in humans and animals is now widely recognized. In animal species, the risk of infection is probably greater than the risk in humans. In human infections associated with biofilm formation were medical device-related infections including Pacemakers, electrical dialysers, joint prosthetics, intravenous catheters, urinary catheter. As food is identified to be a very efficient vehicle for bringing a large number of people into contact with a potential hazard, food processing equipments can be persistent source of spoilage and pathogenic bacteria if microorganisms form biofilm on them. Ideally, preventing biofilm formation would be a more logical option than treating it. The main strategy to prevent biofilm formation is to clean and disinfect regularly before bacteria attach firmly to surfaces. This process can remove 90% or more of microorganisms associated with the surface. Biofilm detectors, acid shock treatment and recently using bacteriophages have been tried. The prevention and control of biofilm formation is somewhat difficult but prevention is the ideal approach even if there are control methods too. The development of better control and prevention methods with better effect need to be given special emphasis.

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Section: Biofilm and Drug Resistancementioning

confidence: 99%

Review on biofilm formation and its control options

2017

Int. J. Adv. Res. Biol. Sci

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“…The biofilm shows maximum resistance to antibiotics in the mature stage due to several mechanisms [99,100] including:…”

Section: Biofilm and Bacterial Advantagesmentioning

confidence: 99%

Biofilm: An Important Bacterial Feature Still to Deal With

Novais

Abreu

Castro

2016

Journal of Life Sciences Research

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Currently, the bacterial infections are one of the main causes of death worldwide and a public health problem for most governments. In this scenario, virulence factors are of importance, including the capacity of forming biofilm. The ability of producing biofilms is directly involved in bacterial pathogenicity and hugely worse the risk of death due to a bacterial infection. This feature allows the bacteria to colonize different surfaces (e.g. Medical devices), and causes several complications, especially in nosocomial infections. This work reviewed biolfim producing mechanisms, the relation with resistance process and the problems associated to biofilms-affected-medical devices.

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“…In the caries formation, the acidogenic potential of microorganisms, their adhesion to dental surfaces and production of intra and extracellular polysaccharides play a key role in the etiopathogenesis 3,4 . Moreover, due to the structural complexity of oral biofilm, microbial physiological features in biofilms 5 , horizontal and vertical transference of mobile genetic determinants, the susceptibility of antimicrobial agents are very limited 6 . In addition, the benefits of the chemical control of dental biofilm are also transients, particularly in prolonged usage 7 and the advantages of chemical control of biofilm in caries prevention has been under scrutiny and side effects are significant and cannot be neglected 8 .…”

Section: Introductionmentioning

confidence: 99%

Influence of subinhibitory concentrations of extracts from Psidium cattleianum (Sabine) and Myracrodruon urundeuva (Allemao) on mutans streptococci adhesion to glass and enamel surfaces.

Bianco¹,

Landucci²,

Schweitzer³

et al. 2017

Arch Health Invest

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This study evaluated the influence of subinhibitory concentrations of extracts from Myracrodruon urundeuva and Psidium cattleianum on the adhesion of cariogenic cocci to the surface of glass and bovine enamel. The aqueous extracts were prepared from leaves by heating in deionized water and sterilized by filtration (22μm). The minimum inhibitory concentration (MIC) of the extracts on S. mutans ATCC 1910 and ATCC 35688 was determined by the broth dilution method. In the tests of adhesion to glass surfaces, glass tubes were treated with saliva and sucrose and the bacterial strains were cultivated in concentrations equivalent to 25% of MIC, which were also maintained in the adhesion assay. After the contact between the cells and the glass, the unbound cocci were removed and, finally, it was performed the removal of the cells adhered to the glass by sonication. The percentage of adhesion was determined by comparing the number of bacteria adhered to the total inoculated bacteria, in the presence and absence of the subinhibitory concentrations of the extracts. For enamel adhesion tests, bovine enamel samples were standardized, polished, treated with saliva and, in the test group, received the bacterial inoculum and 25% of the MIC of the extracts. The enamel blocks were removed and the total of adhered cocci was determined by cultivation on BHI agar supplemented with horse blood in anaerobic conditions for 48h at 37 o C. The results were evaluated through analysis of variance (ANOVA). The extracts were effective in inhibiting bacterial adhesion to glass and dental enamel surfaces, although inhibition varied according to bacterial strain and extract, producing from 40 to 60% of inhibition. The results reinforce the possibility of the use of extracts of plants of the Brazilian savanna in the control of cariogenic biofilms.Descriptors: Bacteria; Prevention & Control; Plant Extracts; Dental Caries. ResumoEste estudo avaliou a influência de concentrações subinibitórias dos extratos de Myracrodruon urundeuva e Psidium cattleianum sobre a adesão de cocos cariogênicos à superfície do vidro e esmalte bovino. Os extratos aquosos foram preparados a partir de folhas, por aquecimento em água deionizada e esterilizados por filtração (22µm). A seguir, a concentração inibitória mínima (CIM) dos extratos sobre S. mutans ATCC 1910 e ATCC 35688 foi determinada através do método de diluição da droga em caldo. Nos testes de aderência ao vidro, tubos eram tratados com saliva e sacarose e as cepas bacterianas eram cultivadas em concentrações equivalentes à 25% da CIM, que também eram mantidas no ensaio de adesão. Após o contato entre as células o do vidro, fazia-se a remoção dos cocos não aderidos e, posteriormente, por sonicação, a remoção das células aderidas ao vidro. A percentagem de adesão era determinada comparando-se o número de bactérias aderidas em relação ao total, na presença e ausência das concentrações subinibitórias dos extratos. Para adesão ao esmalte, amostras de esmalte bovino foram padronizadas, polidas, tratadas com...

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Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies

Cited by 648 publications

References 64 publications

Review on biofilm formation and its control options

Review on biofilm formation and its control options

Biofilm: An Important Bacterial Feature Still to Deal With

Influence of subinhibitory concentrations of extracts from Psidium cattleianum (Sabine) and Myracrodruon urundeuva (Allemao) on mutans streptococci adhesion to glass and enamel surfaces.

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