Bacterial Attraction and Quorum Sensing Inhibition in Caenorhabditis elegans Exudates

Kaplan, Fatma; Badri, Dayakar V.; Zachariah, Cherian; Ajredini, Ramadan; Sandoval, Francisco J.; Roje, Sanja; Levine, Lanfang H.; Zhang, Fengli; Robinette, Steven L.; Alborn, Hans T.; Zhao, Wei; Stadler, Michael; Nimalendran, Rathika; Dossey, Aaron T.; Brüschweiler, Rafael; Vivanco, Jorge M.; Edison, Arthur S.

doi:10.1007/s10886-009-9670-0

Cited by 34 publications

(38 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Males and females were both attracted to the flow-through and 50% methanol (MeOH) fractions, but they did not respond to the 90% fraction. Based on previous studies with C. elegans, the flow-through fraction contains many common metabolites, such as amino acids and sugars (18,19); some of these metabolites are attractive to nematodes (20), whereas the 50% MeOH fraction is enriched in ascarosides (3,5,21). We, therefore, focused our attention on the 50% MeOH fraction, which was further fractionated using ion exchange solid-phase extraction.…”

Section: Resultsmentioning

confidence: 99%

Sex-specific mating pheromones in the nematode Panagrellus redivivus

Choe

Chuman

Reuß

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Nematodes use an extensive chemical language based on glycosides of the dideoxysugar ascarylose for developmental regulation (dauer formation), male sex attraction, aggregation, and dispersal. However, no examples of a female-or hermaphrodite-specific sex attractant have been identified to date. In this study, we investigated the pheromone system of the gonochoristic sour paste nematode Panagrellus redivivus, which produces sex-specific attractants of the opposite sex. Activity-guided fractionation of the P. redivivus exometabolome revealed that males are strongly attracted to ascr#1 (also known as daumone), an ascaroside previously identified from Caenorhabditis elegans hermaphrodites. Female P. redivivus are repelled by high concentrations of ascr#1 but are specifically attracted to a previously unknown ascaroside that we named dhas#18, a dihydroxy derivative of the known ascr#18 and an ascaroside that features extensive functionalization of the lipid-derived side chain. Targeted profiling of the P. redivivus exometabolome revealed several additional ascarosides that did not induce strong chemotaxis. We show that P. redivivus females, but not males, produce the male-attracting ascr#1, whereas males, but not females, produce the female-attracting dhas#18. These results show that ascaroside biosynthesis in P. redivivus is highly sex-specific. Furthermore, the extensive side chain functionalization in dhas#18, which is reminiscent of polyketide-derived natural products, indicates unanticipated biosynthetic capabilities in nematodes.hemical communication plays an important role in the biology of many free-living and parasitic nematodes (1). Recent studies have shown that a family of small-molecule signals, the ascarosides, control sexual attraction (2-5), aggregation (6, 7), olfactory plasticity (8), dauer formation (9-12), and dispersal (13) in the model organism Caenorhabditis elegans. Ascarosides ( Fig. 1) are glycolipids derived from the dideoxysugar ascarylose, which in some cases, bear additional moieties, such as para-aminobenzoic acid and indole carboxy-or p-hydroxybenzoyl groups. The diversity of behavioral effects modulated by ascarosides in C. elegans is paralleled by complexity of ascaroside structures. Targeted metabolomicsbased analysis of wild-type C. elegans exudates (i.e., the exometabolome) resulted in the detection of over 50 ascaroside components, many of which have no known function (14). Using LC-MS analysis, we have previously shown that ascaroside signaling is not restricted to C. elegans and is widely conserved among nematodes (15). Many free-living and parasitic nematode species were shown to release ascarosides. However, the species-specific functions of the identified ascarosides remain largely unknown. Moreover, this targeted analysis focused exclusively on ascarosides previously identified from C. elegans, and it seems likely that other nematode species produce additional ascarosides not present in C. elegans.In this study, we used activity-guided fractionation to isolate both male-an...

show abstract

Section: Resultsmentioning

confidence: 99%

Sex-specific mating pheromones in the nematode Panagrellus redivivus

Choe

Chuman

Reuß

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The nematodes secrete cytokinins that play a role in cell cycle activation and in establishing the feeding site as a nutrient sink in the host roots. Another recent study demonstrated that how nematode secretions (non-proteinaceous compounds) interact with soil-inhabiting bacteria [46 ] by using the model nematode Caenorhabditis elegans. This study will open a new avenue of research to study the chemical interactions of other parasitic nematodes with their hosts.…”

Section: Molecular Communication Between Host and Pathogensmentioning

confidence: 99%

Rhizosphere chemical dialogues: plant–microbe interactions

Badri

Weir

Lelie

et al. 2009

Current Opinion in Biotechnology

532

271

View full text Add to dashboard Cite

“…The ability of roots to communicate with roots of neighboring plants and other organisms in the rhizosphere has been the focus of increasing attention (1,2). Root secretions of secondary metabolites and volatile organic compounds have been shown to play offensive, defensive, and symbiotic roles (3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

De‐la‐Peña

Badri

Lei

et al. 2010

Journal of Biological Chemistry

Self Cite

107

View full text Add to dashboard Cite

Proteins found in the root exudates are thought to play a role in the interactions between plants and soil organisms. To gain a better understanding of protein secretion by roots, we conducted a systematic proteomic analysis of the root exudates of Arabidopsis thaliana at different plant developmental stages. In total, we identified 111 proteins secreted by roots, the majority of which were exuded constitutively during all stages of development. However, defense-related proteins such as chitinases, glucanases, myrosinases, and others showed enhanced secretion during flowering. Defense-impaired mutants npr1-1 and NahG showed lower levels of secretion of defense proteins at flowering compared with the wild type. The flowering-defective mutants fca-1, stm-4, and co-1 showed almost undetectable levels of defense proteins in their root exudates at similar time points. In contrast, root secretions of defense-enhanced cpr5-2 mutants showed higher levels of defense proteins. The proteomics data were positively correlated with enzymatic activity assays for defense proteins and with in silico gene expression analysis of genes specifically expressed in roots of Arabidopsis. In conclusion, our results show a clear correlation between defense-related proteins secreted by roots and flowering time.The plant root system serves many roles, including anchorage and uptake of nutrients and water. The ability of roots to communicate with roots of neighboring plants and other organisms in the rhizosphere has been the focus of increasing attention (1, 2). Root secretions of secondary metabolites and volatile organic compounds have been shown to play offensive, defensive, and symbiotic roles (3-7). Likewise, the symbioses between Rhizobium species and various members of the legume family have been extensively studied and involve the initial secretion of specific flavonoids by plant roots (8 -10). Similarly, a multitrophic interaction has been uncovered, in which volatile organic compounds released by Medicago truncatula were shown to attract soil nematodes that could in turn bring Rhizobia, used as food by the nematodes, to the roots and thus facilitate the symbiotic process (11). All these interactions involve carbon-containing compounds secreted in the root exudates.Although numerous reports show changes in the protein profile of leaves and root tissues in response to wounding (12, 13), insect attack (14), fungal or oomycete infection (15-20), or the defense-related hormone jasmonic acid (21, 22), fewer reports have discussed the biological roles of proteins secreted by the roots (23-25). One recent study has shown that the composition of proteins secreted in the root exudates changes with the presence of a given microbial neighbor and that the exudation of proteins by a given bacterium is altered by the presence of a specific plant neighbor (26). These studies examined root secretion of proteins under inducible conditions, but there is no information available about the protein profiles in the root exudates under normal, nonstressed...

show abstract

Bacterial Attraction and Quorum Sensing Inhibition in Caenorhabditis elegans Exudates

Cited by 34 publications

References 81 publications

Sex-specific mating pheromones in the nematode Panagrellus redivivus

Sex-specific mating pheromones in the nematode Panagrellus redivivus

Rhizosphere chemical dialogues: plant–microbe interactions

Root Secretion of Defense-related Proteins Is Development-dependent and Correlated with Flowering Time

Contact Info

Product

Resources

About