Bacterial Attachment and Detachment in Aluminum‐Coated Quartz Sand in Response to Ionic Strength Change

Lee, Changgu; Park, Seong‐Jik; Han, Yong-Un; Park, Jeong-Ann; Kim, Song-Bae

doi:10.2175/106143009x12529484815593

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…As a result, the effective distance of electrostatic repulsion increases, causing the release of particles reversibly held in secondary energy minima (27). This phenomenon has been well documented for both biological (28) and nonbiological colloids (29) in saturated porous media.…”

Section: Discussionmentioning

confidence: 81%

Assessment of Anaerobic Toluene Biodegradation Activity by bssA Transcript/Gene Ratios

Brow

Johnson

et al. 2013

Appl Environ Microbiol

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Benzylsuccinate synthase (bssA) genes associated with toluene degradation were profiled across a groundwater contaminant plume under nitrate-reducing conditions and were detected in significant numbers throughout the plume. However, differences between groundwater and core sediment samples suggested that microbial transport, rather than local activity, was the underlying cause of the high copy numbers within the downgradient plume. Both gene transcript and reactant concentrations were consistent with this hypothesis. Expression of bssA genes from denitrifying toluene degraders was induced by toluene but only in the presence of nitrate, and transcript abundance dropped rapidly following the removal of either toluene or nitrate. The drop in bssA transcripts following the removal of toluene could be described by an exponential decay function with a half-life on the order of 1 h. Interestingly, bssA transcripts never disappeared completely but were always detected at some level if either inducer was present. Therefore, the detection of transcripts alone may not be sufficient evidence for contaminant degradation. To avoid mistakenly associating basal-level gene expression with actively degrading microbial populations, an integrated approach using the ratio of functional gene transcripts to gene copies is recommended. This approach minimizes the impact of microbial transport on activity assessment and allows reliable assessments of microbial activity to be obtained from water samples.T he use of molecular biological tools has led to significant advancements in the area of in situ bioremediation. Of particulate note is the use of quantitative PCR (qPCR) for the detection and quantification of functional genes associated with the degradation of contaminants. However, as has been repeatedly demonstrated in both column and field studies, functional gene abundance is not always predictive of contaminant degradation, nor does it consistently correlate with gene expression or contaminant concentration (1, 2). Furthermore, high gene copy numbers are often sustained when there is little or no active degradation (3, 4), making such measurements difficult to interpret.In some cases, microbial transport may provide an explanation for elevated gene copies in the absence of degradation. It has been suggested that genes recovered from groundwater samples may represent conditions upgradient of where they were sampled, because planktonic microorganisms are subjected to transport (5). As a result, organisms observed in groundwater samples may not reflect local chemical conditions; rather, their presence may be the result of transport from upstream locations where chemical conditions favored their activity and growth. The impact of microbial transport can be minimized by assessing activity with sediment sampling (6); however, the relative simplicity of groundwater sampling makes it more attractive from a practical standpoint.Functional gene transcripts have been suggested as a more reliable indicator of local contaminant biodegradation,...

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Section: Discussionmentioning

confidence: 81%

Assessment of Anaerobic Toluene Biodegradation Activity by bssA Transcript/Gene Ratios

Brow

Johnson

et al. 2013

Appl Environ Microbiol

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“…ADP was carried out in the presence of different quantities of LDH (10,25,50,75 and 100 mg/mL). Fig.…”

Section: Effect Of Pseudomonas Sp-ldh and Ldh-ha Complex Adhesion Onmentioning

confidence: 99%

“…This prevailing microbial state, known as biofilm, confers protection against environmental stresses and increases their survival [5,6]. Bacteria adsorption was studied and compared on various surfaces [7][8][9][10][11][12][13][14]. The results show that adhesion occurs on all types of surfaces and it is affected by mineral, bacteria cell surface properties and the characteristics of fluid phase (pH, ionic strength).…”

Section: Introductionmentioning

confidence: 99%

“…The results show that adhesion occurs on all types of surfaces and it is affected by mineral, bacteria cell surface properties and the characteristics of fluid phase (pH, ionic strength). As described by DLVO theory of colloid stability [15], two main types of interaction between bacteria and mineral solids are involved: van der Waals attraction and electrostatic interactions that are generally repulsive [10,16]. Besides, bacteria adhesion is influenced by the hydrophobicities of both cells and solids, the bacteria extracellular structures and the presence of divalent cations providing additional bridging.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing atrazine biodegradation by Pseudomonas sp. strain ADP adsorption to Layered Double Hydroxide bionanocomposites

Alekseeva

Prevot

Sancelme

et al. 2011

Journal of Hazardous Materials

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“…According to Derjaguin‐Landaul‐Verwey‐Overbeek (DLVO) theory, the thickness of the electrical double layer of both particles and bacteria should decrease as the ionic strength increases, promoting the close approach of bacteria to particle surfaces (Lee et al . ). For example, the E. coli strain S17 showed a lower negative electrophoretic mobility as ionic strength increased from 1 to 1000 mmol l −1 (Janjaroen et al .…”

Section: Environmental Factors: Ionic Strength Temperature Phmentioning

confidence: 97%

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

et al. 2015

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SummaryEffective modelling of the fate and transport of water-borne pathogens is needed to support federally required pollution-reduction plans, for water quality improvement planning, and to protect public health. Lack of understanding of microbial-particle interactions in water bodies has sometimes led to the assumption that bacteria move in surface waters not associated with suspended mineral and organic particles, despite a growing body of evidence suggesting otherwise. Limited information exists regarding the factors driving interactions between micro-organisms and particles in surface waters. This review discusses cellular, particle and environmental factors potentially influencing interactions and in-stream transport. Bacterial attachment in the aquatic environment can be influenced by properties of the cell such as genetic predisposition and physiological state, surface structures such as flagella and fimbriae, the hydrophobicity and electrostatic charge of the cell surface, and the presence of outer-membrane proteins and extracellular polymeric substances. The mechanisms and degree of attachment are also affected by characteristics of mineral and organic particles including the size, surface area, charge and hydrophobicity. Environmental conditions such as the solution chemistry and temperature are also known to play an important role. Just as the size and surface of chemical particles can be highly variable, bacterial attachment mechanisms are also diverse.

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Bacterial Attachment and Detachment in Aluminum‐Coated Quartz Sand in Response to Ionic Strength Change

Cited by 15 publications

References 27 publications

Assessment of Anaerobic Toluene Biodegradation Activity by bssA Transcript/Gene Ratios

Assessment of Anaerobic Toluene Biodegradation Activity by bssA Transcript/Gene Ratios

Enhancing atrazine biodegradation by Pseudomonas sp. strain ADP adsorption to Layered Double Hydroxide bionanocomposites

Cellular, particle and environmental parameters influencing attachment in surface waters: a review

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