Bacterial Attachment and Biofilm Formation on Antimicrobial Sealants and Stainless Steel Surfaces

Ciolacu, Luminita; Zand, Elena; Negrau, Carmen; Jaeger, Henry

doi:10.3390/foods11193096

Cited by 9 publications

(4 citation statements)

References 79 publications

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“…The interaction with surfaces is essential for the survival and proliferation of bacteria in their natural environment as well as in health and disease. In their natural habitats, most bacteria exist in multicellular ensembles known as biofilms, the establishment of which depends on bacterial attachment to surfaces (83)(84)(85)(86). During bacterial infection of a host, one of the earliest steps in the process is the adhesion of a pathogen to the surfaces of host cells, tissues, and medical implants.…”

Section: Discussionmentioning

confidence: 99%

Surface Hydrophilicity Promotes Bacterial Twitching Motility

O’Hara,

Shimozono,

Dye

et al. 2024

Preprint

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The bacterial type IV pilus (T4P) is a critical virulence factor for many pathogens. These includeAcinetobacter baumanniiandPseudomonas aeruginosa, which are WHO priority pathogens because of their increasing antibiotic resistance. T4P powers bacterial twitching motility which is frequently analyzed by interstitial colony expansion between agar and the polystyrene surfaces of plastic Petri dishes. In such assays with Petri dishes, the twitching motility ofAcinetobacter nosocomialis, an opportunistic human pathogen, was observed with MacConkey but not Luria-Bertani (LB) agar media. One difference between these two media is that bile salts are present as a selective agent in MacConkey but not in LB. We found here that that the addition of bile salts to LB allowedA. nosocomialisto display twitching. Similarly, supplementation of bile salts to LB enhanced the twitching ofA. baumanniiandP. aeruginosa. These observations suggest that there is a common mechanism for bile salts to enhance bacterial twitching to promote interstitial colony expansion. Bile salts are used to inhibit the growth of Gram+ bacteria in MacConkey as they are detergents that can disrupt lipid membranes. Surprisingly, their effects on twitching motility are more related to the alteration of physicochemical properties of twitching surfaces rather than a change in bacterial physiology. This is because bacteria displayed increased twitching motility on hydrophilic surfaces such as those of glass and plasma-treated polystyrene plastics. On such surfaces, twitching motility is no longer stimulated by bile salts. We conclude that the hydrophilicity of surfaces, such as those of animal tissues as well as of medical implants and devices, may significantly impact the functionality of T4P as a virulence factor and its interaction with hosts in the establishment and persistence of a bacterial infection.

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Section: Discussionmentioning

confidence: 99%

Surface Hydrophilicity Promotes Bacterial Twitching Motility

O’Hara,

Shimozono,

Dye

et al. 2024

Preprint

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“…In general, a thicker coating of antimicrobials should prevent biofilm formation. However, the thickness of multiple antimicrobial coatings is weakly correlated with their antimicrobial effects on Staphylococcus capitis biofilms, but this correlation is not seen for Microbacterium lacticum biofilms [117].…”

Section: Surface Coating To Prevent Biofilm Formationmentioning

confidence: 96%

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Prabhukhot,

Eggleton,

Patel

2023

Foods

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Pathogenic biofilm formation within food processing industries raises a serious public health and safety concern, and places burdens on the economy. Biofilm formation on equipment surfaces is a rather complex phenomenon, wherein multiple steps are involved in bacterial biofilm formation. In this review we discuss the stages of biofilm formation, the existing literature on the impact of surface properties and shear stress on biofilms, types of bioreactors, and antimicrobial coatings. The review underscores the significance of prioritizing biofilm prevention strategies as a first line of defense, followed by control measures. Utilizing specific biofilm eradication strategies as opposed to a uniform approach is crucial because biofilms exhibit different behavioral outcomes even amongst the same species when the environmental conditions change. This review is geared towards biofilm researchers and food safety experts, and seeks to derive insights into the scope of biofilm formation, prevention, and control. The use of suitable bioreactors is paramount to understanding the mechanisms of biofilm formation. The findings provide useful information to researchers involved in bioreactor selection for biofilm investigation, and food processors in surfaces with novel antimicrobial coatings, which provide minimal bacterial attachment.

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“…Bacteria within biofilms are typically shielded from environmental stress, antibiotics, and disinfectants . Consequently, the development of antibacterial surfaces capable of impeding microbial adhesion, eradicating bacteria, and preventing biofilm formation has emerged as a prominent research focus in recent years. − …”

Section: Introductionmentioning

confidence: 99%

Preparation and Formation Mechanism Study of Antibiofilm Coating Based on Phase Transition of Glutenin

Chen,

Gao,

Liu

et al. 2024

Biomacromolecules

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The surface of food processing equipment is easily affected by biofilm-forming bacteria, leading to crosscontamination and food safety hazards. The critical issue is how to endow the surface of contact materials with antibacterial and antibiofilm abilities. A sustainable, stable, and antibiofilm coating was prepared by phase transition of glutenin. The disulfide bonds in glutenin were reduced by tris(2-carboxyethyl)phosphine, triggering the phase transition of glutenin. Hydrophobic interactions and intermolecular disulfide bonds may be the primary forces. Furthermore, the phase-transited products formed a nanoscale coating on the surface of stainless steel and glass under their own adhesion force and gravity. The coating exhibited good stability in harsh environments. More importantly, after 3 h of direct contact, the colony of Escherichia coli and Staphylococcus aureus decreased by one logarithm. The amount of biofilm was observed to be significantly decreased through optical microscopy and scanning electron microscopy. This article provides a foundational module for developing novel coatings.

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Bacterial Attachment and Biofilm Formation on Antimicrobial Sealants and Stainless Steel Surfaces

Cited by 9 publications

References 79 publications

Surface Hydrophilicity Promotes Bacterial Twitching Motility

Surface Hydrophilicity Promotes Bacterial Twitching Motility

Multispecies Bacterial Biofilms and Their Evaluation Using Bioreactors

Preparation and Formation Mechanism Study of Antibiofilm Coating Based on Phase Transition of Glutenin

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