Bacterial Associations with Legumes

Peix, Álvaro; Ramírez-Bahena, Martha Helena; Velázquez, Encarna; Bedmar, Eulogio J.

doi:10.1080/07352689.2014.897899

Cited by 298 publications

(200 citation statements)

References 299 publications

Supporting

Mentioning

194

Contrasting

Unclassified

Order By: Relevance

“…Both genera are often isolated from nodules of the Papilionoidae species (Peix et al, 2015). The results suggest that "mulungu" shows a preference for nodulation with α-rhizobia rather than β-rhizobia, though few isolates were evaluated.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Menezes

Escobar

Fraiz

et al. 2017

Rev. Bras. Ciênc. Solo

View full text Add to dashboard Cite

Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from "mulungu" (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both α and β-rhizobia could be found in "mulungu" root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to β-rhizobia could be found for ESA 71 (Burkholderia). All bacterial isolates were effective in colonizing "mulungu" roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in "mulungu" shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA 72, and ESA 75, together with the reference strains, induced increases in the shoot N concentration and total accumulation compared to the absolute control. The results indicate that "mulungu" is able to establish associations with efficient α and β-rhizobia in Brazilian semi-arid soils.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Menezes

Escobar

Fraiz

et al. 2017

Rev. Bras. Ciênc. Solo

View full text Add to dashboard Cite

show abstract

“…denitrification and volatilization), lead to losses that can achieve up to 70% of the N applied (Mortvedt et al, 1999;Signor and Cerri, 2013). Biological nitrogen fixation (BNF) is an alternative to nitrogen supply to several plants, especially legumes due to its association with rhizobia present in roots (Peix et al, 2015). In agriculture, this relationship could be exploited by production and use of inoculants containing efficient strains of rhizobia, and applied directly in seeds or soil (Nogueira and Hungria, 2013).…”

Section: Introductionmentioning

confidence: 99%

Agronomic efficiency of Bradyrhizobium in peanut under different environments in Brazilian Northeast

Sizenando¹,

Ramos²,

Fernandes‐Júnior³

et al. 2016

Afr. J. Agric. Res.

View full text Add to dashboard Cite

Several legumes have natural ability to associate with nitrogen-fixing bacteria known as rhizobia. The efficiency of this association depends on the plant and bacterial genotype and the edaphoclimatic conditions. Peanut is a tropical legume able to associate with a wide range of rhizobia and the selection of efficient bacteria is important to increase the nitrogen fixation in this crop. In order to investigate the agronomic efficiency of two Bradyrhizobium strains, two peanut genotypes were used in field trails carried out in three environments located at Brazilian Northeast. The genotypes (BR1 and L7 Bege) were submitted to rhizobial inoculation (SEMIA 6144 or ESA 123, both Bradyrhizobium strains), and chemical nitrogen fertilization in randomized block design experiments. The following traits were analyzed: flowering (F), main axis height (MAH), number of nodules/plant (NN), number of pods/plant (NP) and weight of pods (WP). Differential responses were found in all to treatments to NN, NP and WP, in the three environments studied. Overall, ESA 123 showed good agronomic performance inducing higher pod production. The results support the evaluation of the Bradyrhizobium in further experiments aiming at its recommendation to commercial inoculants in Brazilian Northeast region.

show abstract

“…Therefore, its use is an eco-friendly approach to reduce the utilization of chemical fertilizers, enhance soil fertility and increase crop production by their biological activity in the rhizosphere. Several bacteria like Pseudomonas, Azospirillum, Azotobacter, Klebsiella, Enterobacter, Alcaligenes, Arthrobacter, Burkholderia, Rhizobium, Flavobacteriaum, Bacillus and Serratia, phosphobacteria and VAM fungi have been used as biofertilizers supplement of nitrogen and phosphorus fertilizers for improved crop production [7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Integrated Effect of Rhizobium and Azotobacter Cultures on the Leguminous Crop Black Gram (Vigna mungo)

Tiwari¹,

Chauhan²,

Singh³

et al. 2017

Adv Crop Sci Tech

View full text Add to dashboard Cite

A pot experiment was performed to evaluate the integrated effect of Rhizobium and Azotobacter sp. on the plant growth, nodule appearance, no of leaf, shoot length, root length, chlorophyll contents and carbohydrate content in .2) and chlorophyll content (1.3 mg/g) and reducing (867.4 µg/g) and non-reducing sugar (1905.5 µg/g) content per plant biomass respectively. The Azotobacter and Rhizobium sp. have friendly associations and they have different physiology and habitat. Therefore, they help plant growth promotion by them own system. Therefore, such combination can be recommended for field application for sustainable agriculture. Excessive application of chemical fertilizers causes environmental and economic problems; hence the use of PGPR and Rhizobium bacteria can be acceptable due to cut contribution expenditure, increase in grain yield and environmental friendly.

show abstract

Bacterial Associations with Legumes

Cited by 298 publications

References 299 publications

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

Agronomic efficiency of Bradyrhizobium in peanut under different environments in Brazilian Northeast

Integrated Effect of Rhizobium and Azotobacter Cultures on the Leguminous Crop Black Gram (Vigna mungo)

Contact Info

Product

Resources

About