Bacterial and Salivary Biomarkers Predict the Gingival Inflammatory Profile

Lee, Angie; Ghaname, Carrie B.; Braun, Thomas; Sugai, James V.; Teles, Ricardo; Loesche, Walter J.; Kornman, Kenneth S.; Giannobile, William V.; Kinney, Janet S.

doi:10.1902/jop.2011.110060

Cited by 65 publications

(72 citation statements)

References 49 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The experimental model of gingivitis was established as a non-invasive model in humans for understanding pathogenesis of gingivitis (Loe et al, 1965;Offenbacher et al, 2009;Grant et al, 2010;Lee et al, 2012). Experiments were conducted at Procter & Gamble (Beijing) Technology Co., Ltd., Oral Care Department, with approval from the P&G Beijing Technical Center (China) Institutional Review Board and in accordance with the World Medical Association Declaration of Helsinki (1996 amendment).…”

Section: Study Design and Sample Collectionmentioning

confidence: 99%

Predictive modeling of gingivitis severity and susceptibility via oral microbiota

Huang

Li²,

Zeng³

et al. 2014

The ISME Journal

121

133

View full text Add to dashboard Cite

Predictive modeling of human disease based on the microbiota holds great potential yet remains challenging. Here, 50 adults underwent controlled transitions from naturally occurring gingivitis, to healthy gingivae (baseline), and to experimental gingivitis (EG). In diseased plaque microbiota, 27 bacterial genera changed in relative abundance and functional genes including 33 flagellar biosynthesis-related groups were enriched. Plaque microbiota structure exhibited a continuous gradient along the first principal component, reflecting transition from healthy to diseased states, which correlated with Mazza Gingival Index. We identified two host types with distinct gingivitis sensitivity. Our proposed microbial indices of gingivitis classified host types with 74% reliability, and, when tested on another 41-member cohort, distinguished healthy from diseased individuals with 95% accuracy. Furthermore, the state of the microbiota in naturally occurring gingivitis predicted the microbiota state and severity of subsequent EG (but not the state of the microbiota during the healthy baseline period). Because the effect of disease is greater than interpersonal variation in plaque, in contrast to the gut, plaque microbiota may provide advantages in predictive modeling of oral diseases.

show abstract

Section: Study Design and Sample Collectionmentioning

confidence: 99%

Predictive modeling of gingivitis severity and susceptibility via oral microbiota

Huang

Li²,

Zeng³

et al. 2014

The ISME Journal

121

133

View full text Add to dashboard Cite

show abstract

“…This, in turn, is further perpetuated by autogenic microbial succession and regulated microbiota interactions [31]. While dysbiosis has also been used to describe disease state due to a shift in the normal resident microbiota, previous studies have examined the changes in the microbiota from different oral habitat over a time period [37,38,39,40,41,42,43]. These studies have taken into account other environmental factors in microbial succession and facilitated the understanding of the microbial interplay.…”

Section: Biofilm and Periodontal Diseasementioning

confidence: 99%

The Biofilm Community: Rebels with a Cause

Aruni

Dou

Mishra

et al. 2015

Curr Oral Health Rep

View full text Add to dashboard Cite

Oral Biofilms are one of the most complex and diverse ecosystem developed by successive colonization of more than 600 bacterial taxa. Development starts with the attachment of early colonizers such as Actinomyces species and oral streptococci on the acquired pellicle and tooth enamel. These bacteria not only adhere to tooth surface but also interact with each other and lay foundation for attachment of bridging colonizer such as Fusobacterium nucleatum followed by late colonizers including the red complex species: Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola-the founders of periodontal disease. As the biofilm progresses from supragingival sites to subgingival sites, the environment changes from aerobic to anaerobic thus favoring the growth of mainly Gram-negative obligate anaerobes while restricting the growth of the early Gram-positive facultative aerobes. Microbes present at supragingival level are mainly related to gingivitis and root-caries whereas subgingival species advance the destruction of teeth supporting tissues and thus causing periodontitis. This review summarizes our present understanding and recent developments on the characteristic features of supra- and subgingival biofilms, interaction between different genera and species of bacteria constituting these biofilms and draws our attention to the role of some of the recently discovered members of the oral community.

show abstract

“…Recently the importance and beneficial role of the oral microbiome in maintaining oral and general health has been brought forward (Marsh, 2012; Hezel and Weitzberg, 2013). On the other hand, undisturbed dental plaque accumulation is associated with an enhanced host inflammatory response and gingival inflammation (gingivitis) (Lee et al, 2012). Gingivitis is known to be associated with the onset of periodontitis (Schatzle et al, 2003), therefore the importance of maintaining gingival health is well understood.…”

Section: Introductionmentioning

confidence: 99%

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Mostajo

Reijden

Buijs

et al. 2014

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Oral bacteria live in symbiosis with the host. Therefore, when mouthwashes are indicated, selective inhibition of taxa contributing to disease is preferred instead of broad-spectrum antimicrobials. The potential selectivity of an oxygenating mouthwash, Ardox-X® (AX), has not been assessed. The aim of this study was to determine the antimicrobial potential of AX and the effects of a twice-daily oral rinse on dental plaque composition.Material and methods: In vitro, 16 oral bacterial strains were tested using agar diffusion susceptibility, minimum inhibitory and minimum bactericidal concentration tests. A pilot clinical study was performed with 25 healthy volunteers. Clinical assessments and microbiological sampling of supragingival plaque were performed at 1 month before the experiment (Pre-exp), at the start of the experiment (Baseline) and after the one-week experimental period (Post-exp). During the experiment individuals used AX mouthwash twice daily in absence of other oral hygiene measures. The microbiological composition of plaque was assessed by 16S rRNA gene amplicon sequencing.Results: AX showed high inter-species variation in microbial growth inhibition. The tested Prevotella strains and Fusobacterium nucleatum showed the highest sensitivity, while streptococci and Lactobacillus acidophilus were most resistant to AX. Plaque scores at Pre-exp and Baseline visits did not differ significantly (p = 0.193), nor did the microbial composition of plaque. During a period of 7-days non-brushing but twice daily rinsing plaque scores increased from 2.21 (0.31) at Baseline to 2.43 (0.39) Post-exp. A significant microbial shift in composition was observed: genus Streptococcus and Veillonella increased while Corynebacterium, Haemophilus, Leptotrichia, Cardiobacterium and Capnocytophaga decreased (p ≤ 0.001).Conclusion: AX has the potential for selective inhibition of oral bacteria. The shift in oral microbiome after 1 week of rinsing deserves further research.

show abstract

Bacterial and Salivary Biomarkers Predict the Gingival Inflammatory Profile

Abstract: In this proof-of-concept investigation, we identified specific biomarker and microbial signatures that are associated with gingival inflammation (ClinicalTrials.gov number NCT00980525).

Cited by 65 publications

References 49 publications

Predictive modeling of gingivitis severity and susceptibility via oral microbiota

Predictive modeling of gingivitis severity and susceptibility via oral microbiota

The Biofilm Community: Rebels with a Cause

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Contact Info

Product

Resources

About