Bacterial and fungal response to nitrogen fertilization in three coniferous forest soils

Demoling, Fredrik; Nilsson, Lars Ola; Bååth, Erland

doi:10.1016/j.soilbio.2007.08.019

Cited by 202 publications

(138 citation statements)

References 69 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…Our findings are consistent with Shen et al (2010) and He et al (2007), who reported decreased soil bacterial abundance related to N fertilization in arable soils. Negative effects of fertilization on bacteria were also reported by Ekblad and Nordgren (2002) and Demoling et al (2008), which indicates that C limitation rather than N limitation affects microbial abundance and activity in coniferous forest soils. Soil organic matter is sensitive to global warming, especially in boreal ecosystems (e.g., Jarvis and Linder 2000;Melillo et al 2002;Davidson and Janssens 2006).…”

Section: Responses Of Aob and Aoa Abundance To Soil Warming And Fertisupporting

confidence: 63%

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

Long

Chen

et al. 2012

J Soils Sediments

View full text Add to dashboard Cite

Purpose Boreal forests are considered to be more sensitive to global climate change compared with other terrestrial ecosystems, but the long-term impact of climate change and forest management on soil microbial functional diversity is not well understood. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are the most important players in nitrogen (N) cycling-associated processes in terrestrial ecosystems. This study investigated the separate and combined impacts of long-term soil warming and fertilization on soil AOB and AOA community structures and abundances in a Norway spruce stand in northern Sweden. Materials and methods The soil-warming experiment was established in the buffer zones of two irrigated plots (I) and complete nutrient solution plots (IL) since 1995. The warming treatment started in April each year by maintaining soil temperature on warmed plots at 5°C above the temperature in unwarmed plots using heating cables. In August 2006, soil samples were collected from eight subplots for molecular analysis. The abundance of bacterial and archaeal amoA genes was determined by quantitative polymerase chain reaction. Similarly, total bacterial and archaeal population sizes have also been determined. The diversity of AOB and AOA was assessed by constructing amoA gene clone libraries, and different genotypes were screened with restriction fragment length polymorphism. Results and discussion Results showed that fertilization did not significantly affect the abundance of the bacterial amoA gene under either warming or non-warming conditions; however, warming decreased the abundance under fertilization treatments. No significant effects of fertilization and soil warming were observed on the number of thaumarchaeal amoA gene copies across all treatments. In this study, amoA gene abundance of AOB was significantly higher than that of AOA across all treatments. The community structure of both AOB and AOA was strongly influenced by fertilization. For bacterial amoA genes, Nitrosospira cluster 2 was present across all treatments, but the only genotype was observed in the fertilization treatments while, for thaumarchaeal amoA genes, the relative abundance of soil cluster 5 increased in fertilization treatments. By comparison, soil-warming effects on AOB and AOA community structure were not significant. Canonical correspondence analysis showed a positive correlation between fertilization and both dominant genotypes of AOB and AOA. Conclusions These results indicated that the abundance of AOA and AOB was not affected by fertilization or warming alone, but the interaction of fertilization and warming reduced the abundance of AOB. The community composition of ammonia-oxidizers was more affected by the nutrientoptimized fertilization than the soil warming.

show abstract

Section: Responses Of Aob and Aoa Abundance To Soil Warming And Fertisupporting

confidence: 63%

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

Long

Chen

et al. 2012

J Soils Sediments

View full text Add to dashboard Cite

show abstract

“…For example, reduced microbial activity has been frequently reported from fertilized forest soils (Compton et al 2004;Demoling et al 2008;Frey et al 2004;Wallenstein et al 2006). Two meta-analysis papers also confirm such a trend, showing that N deposition tends to reduce microbial biomass in the N-poor boreal forest soils (Treseder 2008) and decrease heterotrophic respiration and microbial biomass in N-unlimited temperate forest soils (Janssens et al 2010).…”

Section: N Mineralizationmentioning

confidence: 78%

Effects of nitrogen deposition and fertilization on N transformations in forest soils: a review

et al. 2015

View full text Add to dashboard Cite

Purpose Understanding how process-specific nitrogen (N) transformations in natural forest soils are modified by N deposition and fertilization is critically important to gain mechanistic insights on the links between global N deposition and N enrichment and loss in forest soils. Materials and methods Here we identify the general characteristics and the main mechanisms of N deposition-and fertilization-induced modifications in multiple N transformations, including N immobilization, N mineralization, nitrification (autotrophic nitrification and heterotrophic nitrification), and denitrification, in forest soils by literature survey. Results and discussion Overall, N status, soil C/N ratios, C availability, and soil pH are key factors separately and/or interactively affecting the effects of N deposition and fertilization on forest soil N transformations. In the N-limited stage, N deposition and fertilization can act as a stimulator of N mineralization by removing microbial N limitation and reducing the C/N ratios of the substrate being decomposed. In the Nunlimited stage, N added to forest ecosystems can retard N mineralization, which may primarily be a result of decreased microbial activity due to soil acidification and low C availability. The changes in N mineralization may drive a corresponding change in N immobilization, autotrophic nitrification, and denitrification. Despite the fact that ammonia-oxidizing archaea (AOA) has a higher affinity than ammonia-oxidizers (AOB) for low-concentration ammonia (NH 3 ), low NH 3 availability may still limit the rate of ammonia oxidation (autotrophic nitrification) in acidic forest soils even in the case of high NH 4 + input. Heterotrophic nitrification, however, may be favored if soil C/N ratios and pH decrease with N deposition and fertilization. The responses of denitrification and N 2 O emission to N deposition and fertilization in forests may be nonlinear, with a trend of stimulation in the short term but a decline over time, partly because soil pH has a contrast effect on denitrification capacity and N 2 O emission. Conclusions There are various effects of N deposition and fertilization on forest soil N transformations; thus, their responses to N deposition are still not well characterized and understood. N deposition-and fertilization-induced modifications in soil N transformations have important implications for N enrichment, N loss, and soil acidification in forest ecosystems. In the future, more research is required to investigate on dissimilatory nitrate reduction to ammonium (DNRA) process and link microbial community characteristics and functions of microbial extracellular enzymes with these rate processes in forest soils to narrow the uncertainty in evaluating and predicting ecosystem responses to global N deposition.

show abstract

“…The low autotrophic nitrification potential of subtropical/tropical acidic forest soils suggests that autotrophic nitrification in these soils is unlikely to be stimulated by N deposition. Moreover, soil C: N ratio (Templer et al, 2012) and fungal biomass (Frey et al, 2004;Demoling et al, 2008) are often observed to decrease with N deposition. This indicates that N deposition will not stimulate heterotrophic nitrification to affect NO 3 À production and retention in subtropical/tropical acidic forest soils.…”

Section: Introductionmentioning

confidence: 99%

Are nitrate production and retention processes in subtropical acidic forest soils responsive to ammonium deposition?

Gao

Kou

Yang

et al. 2016

Soil Biology and Biochemistry

View full text Add to dashboard Cite

N tracing model Acid soil of subtropical forest a b s t r a c tChanges in soil N-cycling and retention processes in subtropical/tropical acidic forest ecosystems under anthropogenic N inputs are not well understood. We conducted a laboratory 15 N tracing study on an acid soil (pH values: 4.6 to 5.0) from a subtropical forest fertilized for more than 2.5 years at a rate of 0, 40, and 120 kg NH 4 CleN ha À1 yr À1 , respectively. To get a better resolution of mechanistic changes in soil N cycling and retention processes under NH 4 þ additions, we used a conceptual 15 N tracing model to quantify process-specific and pool-specific N transformation rates in soils. Gross N mineralization rates decreased at high NH 4 þ additions, which were paralleled by a reduction in fungal biomass and mineralization of recalcitrant organic N. Gross NH 4 þ immobilization rates did not show a change with increasing NH 4 þ additions. Interestingly, soil NO 3 À production (heterotrophic, autotrophic, and gross nitrification) and retention (NO 3 À immobilization and dissimilatory nitrate reduction to ammonium) showed insensitivity to increasing additions of NH 4 þ . The mechanisms behind the lack of response of heterotrophic nitrification were unclear, but possibly related to the absence of significant changes in soil C: N ratio and soil acidity under increased NH 4 þ additions. Because of the low autotrophic nitrification potential and the lack of NH 4 þ limitation to autotrophic nitrifiers, autotrophic nitrification was unresponsive to NH 4 þ additions.NO 3 À immobilization rates appeared to be controlled by the NO 3 À produced from heterotrophic nitrification, as indicated by the positive relationship between NO 3 À immobilization and heterotrophic nitri-thus showing a lack of a change under increased NH 4 þ additions. DNRA seemed to be inherently less responsive to environmental changes such as NH 4 þ deposition. Our work demonstrates that enhanced NH 4 þ deposition has a low potential to stimulate soil NO 3 À production and weaken soil retention of NO 3 À in this, and perhaps other subtropical/tropical acidic forest ecosystems.

show abstract

Bacterial and fungal response to nitrogen fertilization in three coniferous forest soils

Cited by 202 publications

References 69 publications

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

Effects of nitrogen deposition and fertilization on N transformations in forest soils: a review

Are nitrate production and retention processes in subtropical acidic forest soils responsive to ammonium deposition?

Contact Info

Product

Resources

About