Bacterial and fungal community structure and diversity in a mining region under long-term metal exposure revealed by metagenomics sequencing

Narendrula-Kotha, Ramya; Nkongolo, K. K.

doi:10.1016/j.egg.2016.11.001

Cited by 58 publications

(36 citation statements)

References 61 publications

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“…While the short term contamination impacts negatively on microbial abundance, richness, and diversity, in aged multi-contaminated soils, the microbial community was found to adapt toward a rich diversity pattern over time (Schimel et al, 2007 ; Paissé et al, 2008 ; Bourceret et al, 2016 ). These findings are in agreement with the present study showing that both microbial diversity and richness in the three aged metal-contaminated soils resembled those found in unperturbed soils (Bourceret et al, 2016 ; Narendrula-Kotha and Nkongolo, 2017 ). Noteworthy, the best richness was found in the soil characterized by the highest As and Hg contents; the positive correlations between these parameters and the relative frequencies of the majority of identified genera suggest that these taxa were well adapted to the long-term contamination.…”

Section: Discussionsupporting

confidence: 93%

“…In this respect, a thorough investigation of fungal communities in these extreme habitats is of paramount importance since fungi represent a significant portion of the biodiversity and biomass in soils and they play key roles in the maintenance of the ecosystem functioning. The use of high throughput next generation sequencing (NGS) has led to a dramatic increase in the resolution and detectable spectrum of diverse fungal phylotypes from a variety of ecosystems including natural environments (Baldrian et al, 2016 ; Mundra et al, 2016 ; Schimann et al, 2017 ) and heavy metal- and PAH-contaminated sites (Bourceret et al, 2016 ; Narendrula-Kotha and Nkongolo, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Fungal Community Structure and As-Resistant Fungi in a Decommissioned Gold Mine Site

et al. 2017

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Although large quantities of heavy metal laden wastes are released in an uncontrolled manner by gold mining activities with ensuing contamination of the surrounding areas, there is scant information on the mycobiota of gold-mine sites. Thus, the present study was aimed to describe the fungal community structure in three differently As-and Hg-polluted soils collected from the Pestarena decommissioned site by using Illumina ® metabarcoding. Fungal richness was found to increase as the contamination level increased while biodiversity was not related to the concentrations of inorganic toxicants. Within the phylum Zygomigota which, irrespective of the contamination level, was predominant in all the soils under study, the most abundant genera were Mucor and Mortierella. The relative abundances of Basidiomycota, instead, tended to raise as the contamination increased; within this phylum the most abundant genera were Cryptococcus and Pseudotomentella. The abundance of Ascomycota, ranging from about 8 to 21%, was not related to the contamination level. The relative abundances of those genera (i.e., Penicillium, Trichoderma, and Chaetomium), the cultivable isolates of which exhibited significant As-resistance, were lower than the set threshold (0.5%). Mass balances obtained from As-exposure experiments with these isolates showed that the main mechanisms involved in counteracting the toxicant were accumulation and, above all, volatilization, the respective extents of which ranged from 0.6 to 5.9% and from 6.4 to 31.2% in dependence of the isolate.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Fungal Community Structure and As-Resistant Fungi in a Decommissioned Gold Mine Site

et al. 2017

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“…Members of Basidiomycota and Glomeromycota were positively correlated with NO 3 and PO 4 concentrations. Trichosporon (Basidiomycota) have been reported to be able to denitrify and could cooperate with bacteria in nitrogen removal [20], while Archaeosporales (Glomeromycota) enhance phosphorous and nitrogen supply to host [48]. Fungi of the phylum Ascomycota were observed to be negatively influenced by pH, which agrees with the findings of Maza-Marquez et al [16], who reported similar findings in wastewater treatment systems.…”

Section: Glomeromycotasupporting

confidence: 86%

“…Metals such as Mg, Sr, Si, and Pb have positively influenced the occurrence of Basidiomycota, Chytridiomycota, and Neocallimastigomycota, while B, Fe, and Zn were correlated with the presence of Ascomycota and Zygomycota, respectively. A previous study [48] reported that metals such as Ni, Co, Mo, V, Fe, Mg, and Mn were the most important parameters influencing microbial composition in the environment.…”

Section: Glomeromycotamentioning

confidence: 96%

Industrial Effluents Harbor a Unique Diversity of Fungal Community Structures as Revealed by High-throughput Sequencing Analysis

Selvarajan¹,

Sibanda²,

Sekar³

et al. 2019

Pol. J. Environ. Stud.

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The actual extent of fungal diversity in different environmental media is still a subject of ongoing research. Little is currently known about the diversity of fungal populations in industrial streams. This study characterized the fungal diversity of different industrial effluents using a high-throughput sequencing approach. A total of 234617 quality filtered reads were obtained from the collected wastewater samples. Phylogenetic taxonomy revealed that resident fungal communities were classified as 6 phyla, 31 classes, 79 orders, 144 families, and 192 genera. Ascomycota and Basidiomycota were the most dominant phyla whose relative abundance ranged from 23.29% to 38.31%, and 17.34% to 33.51%, respectively. Recovered operational taxonomic units (OTUs) ranged from 292 (Dixon) to 427 (Capegate). The existence of some fungal genera identified in the industrial wastewaters correlated to physicochemical variables and had the potential to play important roles in organic decomposition, pollutant degradation, and xenobiotic transformation. Meanwhile, the occurrence of unclassified fungal sequences (22.5% to 33.09%) suggests that these effluents are a potential reservoir of as-yet uncharacterized fungal species.

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“…One study on heavy metal pollution in agricultural land observed that aerobic heterotrophic bacteria had a strong positive correlation with Zn [ 72 ]. Another found that Cu has little influence on major microbial communities in long-term exposure to heavy metal pollution [ 73 ]. Other extensive experiments have shown that Cd stress affected microbial population structure and diversity [ 74 , 75 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Pb Smelting on the Soil Bacterial Community near a Secondary Lead Plant

Luo

Chen

et al. 2018

IJERPH

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Secondary lead smelting is a widespread industrial activity which has exacerbated Pb or Cd contamination of soil and water across the world. Soil physicochemical properties, soil enzyme activities, heavy metal concentrations, and bacterial diversity near a secondary lead plant in Xuzhou, China were examined in this study. The results showed that secondary lead smelting activities influenced nearby soils. Soil acidification decreased one order of magnitude, with a mean value of 7.3. Soil organic matter also showed a downward trend, while potassium and nitrogen appeared to accumulate. Soil urease and protease activity increased in samples with greater heavy metal pollution, but overall the soil microbial biodiversity decreased. Soil heavy metal concentration—especially Pb and Cd—greatly exceeded the concentrations of Chinese Environmental Quality Standard for Soils (GB 15618-1995). Some environmental factors—such as pH, organic matter, enzyme activity, and the concentration of heavy metals—significantly affected bacterial diversity: compared with the control site, the Chao1 estimator decreased about 50%, while the Shannon diversity index dropped approximately 20%. Moreover, some genera have significant relationships with heavy metal concentration—such as Ramlibacter with Zn and Steroidobacter with Cd—which might act as bio-indicators for soil remediation. These results will provide a new insight in the future for reclaiming soil contaminants caused by secondary lead smelting.

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Bacterial and fungal community structure and diversity in a mining region under long-term metal exposure revealed by metagenomics sequencing

Cited by 58 publications

References 61 publications

Fungal Community Structure and As-Resistant Fungi in a Decommissioned Gold Mine Site

Fungal Community Structure and As-Resistant Fungi in a Decommissioned Gold Mine Site

Industrial Effluents Harbor a Unique Diversity of Fungal Community Structures as Revealed by High-throughput Sequencing Analysis

Effects of Pb Smelting on the Soil Bacterial Community near a Secondary Lead Plant

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