Bacterial and Cellular Response to Yellow-Shaded Surface Modifications for Dental Implant Abutments

Abstract

Dental implants have dramatically changed the rehabilitation procedures in dental prostheses but are hindered by the possible onset of peri-implantitis. This paper aims to assess whether an anodization process applied to clinically used surfaces could enhance the adhesion of fibroblasts and reduce bacterial adhesion using as a reference the untreated machined surface. To this purpose, four different surfaces were prepared: (i) machined (MAC), (ii) machined and anodized (Y-MAC), (iii) anodized after sand-blasting and acid etching treatment (Y-SL), and (iv) anodized after double acid etching (Y-DM). All specimens were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Moreover, the mean contact angle in both water and diiodomethane as well as surface free energy calculation was assessed. To evaluate changes in terms of biological responses, we investigated the adhesion of Streptococcus sanguinis (S. sanguinis) and Enterococcus faecalis (E. faecalis), fetal bovine serum (FBS) adsorption, and the early response of fibroblasts in terms of cell adhesion and viability. We found that the anodization reduced bacterial adhesion, while roughened surfaces outperformed the machined ones for protein adsorption, fibroblast adhesion, and viability independently of the treatment. It can be concluded that surface modification techniques such as anodization are valuable options to enhance the performance of dental implants.

Efficacy of a Solution Containing 33% Trichloroacetic Acid and Hydrogen Peroxide in Decontaminating Machined vs. Sand-Blasted Acid-Etched Titanium Surfaces

“…To quantify the protein adsorbed on the MAC and SBAE surfaces, the samples were incubated in the presence of fetal bovine serum (FBS) (2% in phosphate buffered saline (PBS)) at 37 • C for 30 min, and then they were washed twice with PBS. As described elsewhere [46], the total protein amount was first eluted from the samples with Tris Triton buffer (10 mM Tris (pH 7.4), 100 mM NaCl, 1 mM EDTA, 1 mM EG-TA, 1% Triton X-100, 10% glycerol and 0.1% SDS) for 10 min, then quantified using a Pierce™ BCA Protein Assay Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions using the spectrophotometer "Jenway 6300" (Jenway, London, UK).…”