Bacterial alginate production: an overview of its biosynthesis and potential industrial production

Urtuvia, Viviana; Maturana, Nataly; Acevedo, Fernando; Peña, Carlos; Díaz‐Barrera, Alvaro

doi:10.1007/s11274-017-2363-x

Cited by 115 publications

(91 citation statements)

References 58 publications

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“…Its block-like structure is organized in the pattern of homogenous (poly-G, poly-M) or heterogeneous (MG) pattern [7]. Because of the specific profiles of the monomers and their modes of linkage in the polymer, the geometries of the G-block regions, M-block regions, and alternating regions are considerably diverse [8]. Increasing G blocks in alginate and the molecular weight of the polymer can form stronger or fragile ALG gels.…”

Section: Chemical Structurementioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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Hydrogels are a three-dimensional and crosslinked network of hydrophilic polymers. They can absorb a large amount of water or biological fluids, which leads to their swelling while maintaining their 3D structure without dissolving (Zhu and Marchant, Expert Rev Med Devices 8:607-626, 2011). Among the numerous polymers which have been utilized for the preparation of the hydrogels, polysaccharides have gained more attention in the area of pharmaceutics; Sodium alginate is a non-toxic, biocompatible, and biodegradable polysaccharide with several unique physicochemical properties for which has used as delivery vehicles for drugs (Kumar Giri et al., Curr Drug Deliv 9:539-555, 2012). Owing to their high-water content and resembling the natural soft tissue, hydrogels were studied a lot as a scaffold. The formation of hydrogels can occur by interactions of the anionic alginates with multivalent inorganic cations through a typical ionotropic gelation method. However, those applications require the control of some properties such as mechanical stiffness, swelling, degradation, cell attachment, and binding or release of bioactive molecules by using the chemical or physical modifications of the alginate hydrogel. In the current review, an overview of alginate hydrogels and their properties will be presented as well as the methods of producing alginate hydrogels. In the next section of the present review paper, the application of the alginate hydrogels will be defined as drug delivery vehicles for chemotherapeutic agents. The recent advances in the application of the alginate-based hydrogels will be describe later as a wound dressing and bioink in 3D bioprinting.

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Section: Chemical Structurementioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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“…An alternative means of alginate production is through bacterial biosynthesis (Hay et al, 2013). This process is mediated largely by the species of the genera Pseudomonas and Azotobacter (Hay et al, 2013;Moradali et al, 2017;Urtuvia et al, 2017) and is a costly biofabrication process with a theoretical risk of pathogenicity.…”

Section: Plant-derived Biomaterials Alginatementioning

confidence: 99%

Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications

et al. 2019

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The pursuit of appropriate, biocompatible materials is one of the primary challenges in translational bioprinting. The requirement to refine a biomaterial into a bioink places additional demands on the criteria for candidate biomaterials. The material must enable extrusion as a liquid bioink and yet be capable of maintaining its shape in the post-printing phase to yield viable tissues, organs and biological materials. Plant-derived biomaterials show great promise in harnessing both the natural strength of plant microarchitecture combined with their natural biological roles as supporters of cell growth. The aim of this review article is to outline the most widely used biomaterials derived from land plants and marine algae: nanocellulose, pectin, starch, alginate, agarose, fucoidan, and carrageenan, with an in-depth focus on nanocellulose and alginate. The properties that render these materials as promising bioinks for three dimensional bioprinting is herein discussed alongside their potential in 3D bioprinting for tissue engineering, drug delivery, wound healing, and implantable medical devices.

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“…The precursor for the alginate monomers is fructose 6-phosphate, so alginate biosynthesis is closely connected to the central carbohydrate metabolism of the cells (Maleki et al, 2015(Maleki et al, , 2017Ertesvåg et al, 2017). Alginate production is furthermore an energy demanding process and is tightly controlled by a complex network of regulators which also influence other cellular processes (Urtuvia et al, 2017). The state of several cellular processes can therefore be expected to affect the biosynthesis of alginate.…”

Section: Introductionmentioning

confidence: 99%

Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library

Jakobsen

Sletta

Klinkenberg

et al. 2020

Front. Bioeng. Biotechnol.

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Azotobacter vinelandii produces the biopolymer alginate, which has a wide range of industrial and pharmaceutical applications. A random transposon insertion mutant library was constructed from A. vinelandii ATCC12518Tc in order to identify genes and pathways affecting alginate biosynthesis, and about 4,000 mutant strains were screened for altered alginate production. One mutant, containing a mucA disruption, displayed an elevated alginate production level, and several mutants with decreased or abolished alginate production were identified. The regulatory proteins AlgW and AmrZ seem to be required for alginate production in A. vinelandii, similarly to Pseudomonas aeruginosa. An algB mutation did however not affect alginate yield in A. vinelandii although its P. aeruginosa homolog is needed for full alginate production. Inactivation of the fructose phosphoenolpyruvate phosphotransferase system protein FruA resulted in a mutant that did not produce alginate when cultivated in media containing various carbon sources, indicating that this system could have a role in regulation of alginate biosynthesis. Furthermore, impaired or abolished alginate production was observed for strains with disruptions of genes involved in peptidoglycan biosynthesis/recycling and biosynthesis of purines, isoprenoids, TCA cycle intermediates, and various vitamins, suggesting that sufficient access to some of these compounds is important for alginate production. This hypothesis was verified by showing that addition of thiamine, succinate or a mixture of lysine, methionine and diaminopimelate increases alginate yield in the non-mutagenized strain. These results might be used in development of optimized alginate production media or in genetic engineering of A. vinelandii strains for alginate bioproduction.

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Bacterial alginate production: an overview of its biosynthesis and potential industrial production

Cited by 115 publications

References 58 publications

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications

Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library

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