Bacterial alginate biosynthesis – recent progress and future prospects

Gacesa, Peter

doi:10.1099/00221287-144-5-1133

Cited by 171 publications

(130 citation statements)

References 95 publications

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“…Components of the polysaccharide capsule, such as the exopolysaccharide alginate, play a role in both adhesion and protection of the bacterial cells from external stress (52). Among plant pathogenic pseudomonads, alginate production has been linked to epiphytic fitness and the production of watersoaked lesions (53).…”

Section: Ttss and Effectorsmentioning

confidence: 99%

The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

Buell

Joardar

Lindeberg

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

773

643

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We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.

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Section: Ttss and Effectorsmentioning

confidence: 99%

The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

Buell

Joardar

Lindeberg

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

773

643

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“…Likely each of these properties contributes to the ability of mucoid P. aeruginosa to persist and establish chronic infections in the CF lung. Since aspects regarding the genetics, pathogenesis, and biochemistry of alginate have been reviewed elsewhere [4,8,9], we focus attention on observations regarding the role of alginate in biofilms of mucoid P. aeruginosa.…”

Section: Alginatementioning

confidence: 99%

Role of polysaccharides in Pseudomonas aeruginosa biofilm development

Ryder

Byrd

Wozniak

2007

Current Opinion in Microbiology

508

385

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During the past decade, there has been a renewed interest in using P. aeruginosa as a model system for biofilm development and pathogenesis. Since the biofilm matrix represents a critical interface between the bacterium and the host or its environment, considerable effort has been expended to acquire a more complete understanding of the matrix composition. Here, we focus on recent developments regarding the roles of alginate, Psl, and Pel polysaccharides in the biofilm matrix.

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“…There are 24 genes located on the bacterial chromosome, involved in the production and secretion of alginate in P. aeruginosa [45]. Eight genes are implicated in the exportation of alginic acids (Figure 4), and twelve in the biosynthesis of the polysaccharide [47].…”

Section: Bacterial Alginatesmentioning

confidence: 99%

“…Alginate is an exopolysaccharide with a relatively high molecular mass (10 4 -10 6 g/ml). It consists of the uronic acid residues -D-mannuronate (M) and its C-5 epimer, -L-guluronate (G) [45] (Figure 3). Generally, the monomers form a block copolymer with homopolymeric regions of poly--Dmannuronate (M-blocks) and poly--L-guluronate (G-blocks) as well as heteropolymeric regions (MG-blocks).…”

Section: Bacterial Alginatesmentioning

confidence: 99%