Bacterial adhesion to braided surgical sutures: an in vitro study

Boybeyi, Özlem; Kaçmaz, Birgül; Günal, Yasemin Dere; Gül, Serdar; Yörübulut, Serap; Aslan, Mustafa

doi:10.1007/s00238-015-1171-5

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…Moreover, larger surface area and serrated surface of multifilament sutures constitute a complex three-dimensional surface topography which provides more adequate surface for bacterial adhesion [ 3 ]. Additionally, multifilament sutures were reported to be more susceptible for microbial colonization compared to monofilament sutures [ 3 , 15 ]. Thus, within 7-minutes of the NTAP treatment period, multifilament sutures would be modified to a larger extent, in comparison to multifilament sutures.…”

Section: Discussionmentioning

confidence: 99%

“…Infection on the site of a surgical wound not only jeopardizes the desired wound healing process, but might also evolve into life- threatening conditions, especially in critically ill patients [ 12 , 14 ]. Similar to other implanted medical device-related infections, biofilm formation is the key process for the pathogenesis of suture-related SSIs [ 2 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections

et al. 2018

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Surgical site infections have a remarkable impact on morbidity, extended hospitalization and mortality. Sutures strongly contribute to development of surgical site infections as they are considered foreign material in the human body. Sutures serve as excellent surfaces for microbial adherence and subsequent colonization, biofilm formation and infection on the site of a surgery. Various antimicrobial sutures have been developed to prevent suture-mediated surgical site infection. However, depending on the site of surgery, antimicrobial sutures may remain ineffective, and antimicrobial agents on them might have drawbacks. Plasma, defined as the fourth state of matter, composed of ionized gas, reactive oxygen and nitrogen species, free radical and neutrals, draws attention for the control and prevention of hospital-acquired infections due to its excellent antimicrobial activities. In the present study, the efficacy of non-thermal atmospheric plasma treatment for prevention of surgical site infections was investigated. First, contaminated poly (glycolic-co-lactic acid), polyglycolic acid, polydioxanone and poly (glycolic acid-co-caprolactone) sutures were treated with non-thermal atmospheric plasma to eradicate contaminating bacteria like Staphylococcus aureus and Escherichia coli. Moreover, sutures were pre-treated with non-thermal atmospheric plasma and then exposed to S. aureus and E. coli. Our results revealed that non-thermal atmospheric plasma treatment effectively eradicates contaminating bacteria on sutures, and non-thermal atmospheric plasma pre-treatment effectively prevents bacterial colonization on sutures without altering their mechanical properties. Chemical characterization of sutures was performed with FT-IR and XPS and results showed that non-thermal atmospheric plasma treatment substantially increased the hydrophilicity of sutures which might be the primary mechanism for the prevention of bacterial colonization. In conclusion, plasma-treated sutures could be considered as novel alternative materials for the control and prevention of surgical site infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections

et al. 2018

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“…The rapid increase in research on nanofibers has led to the consideration of nanofibers as potential candidates for wound dressing application [ 49 ]. Nanofiber sutures have also gained research interest because of their inherent advantage of being able to prevent adherence of bacteria to the filaments as a consequence of their size [ 100 ], with wider diameter sutures having a higher risk of bacterial adherence [ 101 ]. Nanofiber sutures produce less tissue reactivity, mimic collagen fibers in the extracellular tissue matrix, and provide the cells with a native environment at the wound site with a reduced inflammatory response.…”

Section: Accelerating Healing Of Surgical Woundsmentioning

confidence: 99%

Applications of Electrospun Drug-Eluting Nanofibers in Wound Healing: Current and Future Perspectives

Akombaetwa¹,

Bwanga²,

Makoni

et al. 2022

Polymers

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Wounds are a consequence of disruption in the structure, integrity, or function of the skin or tissue. Once a wound is formed following mechanical or chemical damage, the process of wound healing is initiated, which involves a series of chemical signaling and cellular mechanisms that lead to regeneration and/or repair. Disruption in the healing process may result in complications; therefore, interventions to accelerate wound healing are essential. In addition to mechanical support provided by sutures and traditional wound dressings, therapeutic agents play a major role in accelerating wound healing. The medicines known to improve the rate and extent of wound healing include antibacterial, anti-inflammatory, and proliferation enhancing agents. Nonetheless, the development of these agents into eluting nanofibers presents the possibility of fabricating wound dressings and sutures that provide mechanical support with the added advantage of local delivery of therapeutic agents to the site of injury. Herein, the process of wound healing, complications of wound healing, and current practices in wound healing acceleration are highlighted. Furthermore, the potential role of drug-eluting nanofibers in wound management is discussed, and lastly, the economic implications of wounds as well as future perspectives in applying fiber electrospinning in the design of wound dressings and sutures are considered and reported.

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“…In terms of biocompatibility passive strategies are preferred, though active strategies are often more effective in spite of toxicity issue and the development of resistant microbial strains compromise their application. 114 Commonly, active strategies are related to preventive approaches which include antimicrobial ion (e.g., silver-ion), prophylactic antibiotics before the biofilm can form and potential antiseptics (e.g., chlorhexidine, polyhexamethylene biguanide, nitrofurazone, 116 octenidine, 117 and triclosan). 118 The antimicrobial properties of the silver ion have been exploited for a long time.…”

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confidence: 99%

Study of the monofilar suture based on the segmented copolymer GL-b-(GL-co-TMC-co-CL-)-b-GL thermal behavior, degradability and incorporation of active agents : polymers and biopolymers

Márquez Lobato

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Biomaterials based on block segmented copolymers constituted by hard and soft segments have attracted much attention for biomedical applications in the last decades, specifically as absorbable monofilament sutures. Polyglycolide has demonstrated to be a good candidate to constitute the hard segment due to its biodegradability and high stiffness, whereas the incorporation of different monomers (e.g. -caprolactone, trimethylene carbonate or glycolide) in a random distribution as soft segment promotes material flexibility and enhances in vitro degradation rates. Resulting material properties can be tailored by the combination of different ratios of hard and soft segments and the specific monomer composition. In this Thesis the study of a tricomponent segmented copolymer commercially marketed as Monosyn® by B. Braun Surgical as an absorbable monofilament suture is presented. The main goals of the work concern to: a) the complete physical characterization that involves the study of the crystallization kinetics and thermal stability, b) the study of the degradation behavior and the associated microstructural changes, c) the evaluation of the capability to improve the performance of Monosyn® by adding compounds with pharmacological activity and d) the production of nanofibers to be employed as reinforcing agents. Isothermal and non-isothermal crystallizations were followed by different techniques (e.g. optical microscopy, differential scanning calorimetry and time-resolved SAXS experiments in a synchrotron radiation facility). The composition of the soft segment influenced in the crystallization of the segmented copolymer as could be deduced by comparison with reported data on similar bicomponent systems. Hydrolytic degradation was carried out at different temperatures and in buffered media of a continuous range of pH. Surface morphology of hydrolyzed sutures showed the formation of longitudinal and circumferential cracks in the outer and inner part of the suture, respectively. These fractures were associated to the existence of interfibrillar and interlamellar amorphous domains as was revealed by SAXS experiments. To explore the potential applications of Monosyn® as an absorbable monofilament suture with pharmacological activity, different drugs were incorporated. Moreover, the use of an amorphous copolymer coating was also evaluated. The first group of drugs selected were biguanide compounds (i.e. chlorhexidine and polyhexametilen biguanide), which have a well-recognized bactericide activity. Specifically, the effectiveness of using a coating was proved as well as the influence of drug molecular size in the activity. The study of the loading process, release behavior and pharmacological activity was completed considering an antibiotic (chloramphenicol) and a healing agent (captopril). Finally, nanofibers of the selected copolymer were prepared by means of the electrospinning technique. These nanofibers were also loaded with pharmacological drugs and used as reinforcing agent of biodegradable polymer matrices. In order to obtain appropriate fabrics different fiber compositions and electrospun set up configurations were tested. En las últimas décadas, los biomateriales basados en copolímeros segmentados, constituidos por segmentos duros y blandos, han atraído el un creciente interés para una amplia gama de s aplicaciones biomédicas, especialmente como suturas monofilares absorbibles. Se ha demostrado que la poliglicolida es una buena candidata para constituir el segmento duro debido a su biodegradabilidad y a su alta rigidez, mientras que la incorporación de distintos monómeros (por ejemplo, -caprolactona, carbonato de trimetileno o glicolida) en una disposición estadística como segmento blando promueven la flexibilidad del material y mejoran la velocidad de degradación in vitro. Las propiedades de los materiales resultantes pueden ajustarse combinando diferentes proporciones de los segmentos duro y blando, y con una composición específica de monómero. En esta Tesis se presenta el estudio del copolímero segmentado tricomponente comercializado por B.Braun Surgical como sutura absorbible monofilar con el nombre de Monosyn®. Los principales objetivos de este trabajo son: a) la caracterización física completa que incluye el estudio de estabilidad térmica y cinéticas de cristalización, b) el estudio de la degradación y los cambios microestructurales asociados, c) la evaluación de la mejora del uso de la sutura añadiendo componentes con actividad farmacológica, y d) la producción de nanofibras para utilizarlas como agentes reforzantes. Las cristalizaciones isotérmicas y no isotérmicas se siguieron mediante distintas técnicas (microscopía óptica, calorimetría diferencial de barrido y experimentos de difracción de rayos X de bajo ángulo). La composición del segmento blando influyó en la cristalización del copolímero segmentado, como era de esperar por la comparación con los datos publicados del sistema bicomponente similar. La degradación hidrolítica fue llevada a cabo a distintas temperaturas con disoluciones tamponadas en un rango continuo de pH. La morfología de la superficie de las suturas hidrolizadas mostró la formación de fracturas longitudinales y circunferenciales en la zona externa e interna de la sutura, respectivamente. Estas fracturas se asociaron a la existencia de dominios amorfos interfibrilares e interlamelares, tal y como se corroboró mediante los experimentos de difracción de rayos X de bajo ángulo. Para explorar las aplicaciones potenciales de Monosyn® como sutura absorbible monofilar con actividad farmacológica, se incorporaron distintos fármacos. Además, se evaluó el uso de un copolímero amorfo como recubrimiento. El primer grupo de fármacos seleccionados corresponden a la familia de las biguanidas (clorhexidina y polihexametilen biguanida), conocidas por su actividad bactericida. En particular se probó la efectividad de utilizar un recubrimiento y la influencia del tamaño molecular del fármaco en su actividad. El estudio del proceso de carga, el perfil de liberación y la actividad farmacológica se completó considerando también un antibiótico (cloranfenicol) y un agente cicatrizante (captopril). Por último, se prepararon nanofibras del copolímero seleccionado mediante la técnica del electrohilado. Estas nanofibras se cargaron con fármaco y se utilizaron como agentes reforzantes de matrices poliméricas biodegradables. Con el propósito de conseguir unos tejidos apropiados, se probaron distintas composiciones de fibras y diferentes configuraciones en el montaje del sistema de electrohilado.

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Bacterial adhesion to braided surgical sutures: an in vitro study

Cited by 4 publications

References 14 publications

Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections

Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections

Applications of Electrospun Drug-Eluting Nanofibers in Wound Healing: Current and Future Perspectives

Study of the monofilar suture based on the segmented copolymer GL-b-(GL-co-TMC-co-CL-)-b-GL thermal behavior, degradability and incorporation of active agents : polymers and biopolymers

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