Bacterial Adhesion on the Titanium and Stainless-Steel Surfaces Undergone Two Different Treatment Methods: Polishing and Ultrafast Laser Treatment

Chik, N; Zain, W S Wan Md; Mohamad, Ahmad Johari; Sidek, Mohd Zaidi; Ibrahim, Wan Hashim Wan; Reif, A; Rakebrandt, J.-H.; Pfleging, Wilhelm; Liu, Xianping

doi:10.1088/1757-899x/358/1/012034

Cited by 14 publications

(8 citation statements)

References 11 publications

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“…As illustrated in Figure 3, the distance from the center of the cell to the end of neurite (the orange line in Figure 3B) represents the length of neurite ( L n ), the area of cell contour (the blue grid in Figure 3B) represents the spreading area ( S s ) of the cell, and the number of live cells ( N l ) and dead cells ( N d ) can be obtained. The cell adhesion rate was defined as the number of live PC12 cells on substrate per cm 2 [25,26]. Then the average length of neurite, the cell spreading area, the cell viability and the cell adhesion rate of each substrate were calculated.…”

Section: Methodsmentioning

confidence: 99%

The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells

Xiang

Fang

et al. 2019

Bioscience Reports

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Recent studies have shown the importance of cell–substrate interaction on neurone outgrowth, where the Young’s modulus of the matrix plays a crucial role on the neurite length, migration, proliferation, and morphology of neurones. In the present study, PC12 cells were selected as the representative neurone to be cultured on hydrogel substrates with different stiffness to explore the effect of substrate stiffness on the neurone outgrowth. By adjusting the concentration of gelatin methacryloyl (GelMA), the hydrogel substrates with the variation of stiffnesses (indicated by Young’s modulus) from approximately 3–180 KPa were prepared. It is found that the stiffness of GelMA substrates influences neuronal outgrowth, including cell viability, adhesion, spreading, and average neurite length. Our results show a critical range of substrate’s Young’s modulus that support PC12 outgrowth, and modulate the cell characteristics and morphology. The present study provides an insight into the relationship between the stiffness of GelMA hydrogel substrates and PC12 cell outgrowth, and helps the design and optimization of tissue engineering scaffolds for nerve regeneration.

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Section: Methodsmentioning

confidence: 99%

The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells

Xiang

Fang

et al. 2019

Bioscience Reports

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“…Inspired by natural lotus leaves [ 2 ], rose petals [ 3 ], and butterfly wings [ 4 ], super-hydrophobic surfaces (i.e., apparent contact angle above 150° and sliding angle below 10°) have been successfully mimicked through the synergetic effects of micro/nanostructure fabrication and surface chemical modification [ 5 , 6 , 7 , 8 , 9 , 10 ]. Due to their enormous potential applications including anti-icing [ 11 ], drag reduction [ 12 ], self-cleaning [ 13 ], anti-bacteria [ 14 ], and corrosion resistance [ 15 ], super-hydrophobic surface mimicry has been extensively developed by state-of-the-art techniques, such as thermal imprinting [ 16 , 17 ], chemical vapor deposition [ 18 ], coating [ 19 ], electrochemical deposition [ 20 , 21 ], and laser texturing [ 22 , 23 , 24 , 25 , 26 , 27 ]. Particularly, laser texturing can be seen as one of the facile approaches, and therefore can be extensively utilized to fabricate super-hydrophobic substrates owing to its precise control of surface fabrication with three dimensional (3D) hierarchical structures [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Droplet Impact on the Super-Hydrophobic Surface with Micro-Pillar Arrays Fabricated by Hybrid Laser Ablation and Silanization Process

et al. 2019

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A super-hydrophobic aluminum alloy surface with decorated pillar arrays was obtained by hybrid laser ablation and further silanization process. The as-prepared surface showed a high apparent contact angle of 158.2 ± 2.0° and low sliding angle of 3 ± 1°. Surface morphologies and surface chemistry were explored to obtain insights into the generation process of super-hydrophobicity. The main objective of this current work is to investigate the maximum spreading factor of water droplets impacting on the pillar-patterned super-hydrophobic surface based on the energy conservation concept. Although many previous studies have investigated the droplet impacting behavior on flat solid surfaces, the empirical models were proposed based on a few parameters including the Reynolds number (Re), Weber number (We), as well as the Ohnesorge number (Oh). This resulted in limitations for the super-hydrophobic surfaces due to the ignorance of the geometrical parameters of the pillars and viscous energy dissipation for liquid flow within the pillar arrays. In this paper, the maximum spreading factor was deduced from the perspective of energy balance, and the predicted results were in good agreement with our experimental results with a mean error of 4.99% and standard deviation of 0.10.

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“…The stability of air inside valleys is not completely understood which, coupled with contradictory reports highlight that wettability may not be a clear indicator of bacterial attachment. In fact, other researchers have shown that its influence may be dependent on the wettability of both surface and bacterial strain, leading to a rule of thumb for which hydrophobic bacteria adhere predominantly to hydrophobic surfaces and vice-versa. ,, Available literature regards S. epidermidis as a hydrophilic species; thus, its affinity with water would result in a surrounding layer of adsorbed water, limiting further contact and adhesion to hydrophobic materials, as proposed by van Loosdrecht et al .…”

Section: Resultsmentioning

confidence: 58%

Surface Free Energy Dominates the Biological Interactions of Postprocessed Additively Manufactured Ti-6Al-4V

Villapún

Carter

Schröder

et al. 2022

ACS Biomater. Sci. Eng.

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Additive manufacturing (AM) has emerged as a disruptive technique within healthcare because of its ability to provide personalized devices; however, printed metal parts still present surface and microstructural defects, which may compromise mechanical and biological interactions. This has made physical and/or chemical postprocessing techniques essential for metal AM devices, although limited fundamental knowledge is available on how alterations in physicochemical properties influence AM biological outcomes. For this purpose, herein, powder bed fusion Ti-6Al-4V samples were postprocessed with three industrially relevant techniques: polishing, passivation, and vibratory finishing. These surfaces were thoroughly characterized in terms of roughness, chemistry, wettability, surface free energy, and surface ζ-potential. A significant increase in Staphylococcus epidermidis colonization was observed on both polished and passivated samples, which was linked to high surface free energy donor γ– values in the acid–base, γAB component. Early osteoblast attachment and proliferation (24 h) were not influenced by these properties, although increased mineralization was observed for both these samples. In contrast, osteoblast differentiation on stainless steel was driven by a combination of roughness and chemistry. Collectively, this study highlights that surface free energy is a key driver between AM surfaces and cell interactions. In particular, while low acid–base components resulted in a desired reduction in S. epidermidis colonization, this was followed by reduced mineralization. Thus, while surface free energy can be used as a guide to AM device development, optimization of bacterial and mammalian cell interactions should be attained through a combination of different postprocessing techniques.

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Bacterial Adhesion on the Titanium and Stainless-Steel Surfaces Undergone Two Different Treatment Methods: Polishing and Ultrafast Laser Treatment

Cited by 14 publications

References 11 publications

The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells

The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells

Droplet Impact on the Super-Hydrophobic Surface with Micro-Pillar Arrays Fabricated by Hybrid Laser Ablation and Silanization Process

Surface Free Energy Dominates the Biological Interactions of Postprocessed Additively Manufactured Ti-6Al-4V

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