Bacterial adhesion on fissure sealants: Effects of exposure to acidic drink

Abstract

BackgroundAdherence of bacteria to teeth surface is considered an important step in the development of caries and the use of fissure sealants is crucial for the prevention of caries in occlusal surfaces of molars and premolars. The aim of this study was to investigate and compare the adherence of Streptococcus mutans to different fissure sealants, after acidic drink exposure.Material and MethodsThe tested materials were Fissurit, Fissurit FX, Grandio Seal, Fuji Triage, Constic. Bacterial suspension was deposited onto each material and the adhesion was evaluated trough the colony forming units (CFUs) determination with or without acidic drink exposure.ResultsThe tested materials showed different behaviors with significant differences. Bacterial adherence values of the untreated materials were very dissimilar: Fuji Triage and Constic materials showed the better results (P<0.05).ConclusionsSurface alteration after acidic drink exposure, changed the bacterial adhesion (except for Grandio Seal): Fissurit, Fissurit FX and Fuji Triage decreased their susceptibility to be colonized by S. mutans (P<0.05); on the contrary, Constic increased up to ~4 times the bacterial adhesiveness respected to the untreated control (P<0.05). Key words:Acidic drinks, bacterial adhesion, fissure sealants, Streptococcus mutans.

Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer

“…Furthermore, it is important to highlight that the type of vehicle affects diffusion and antimicrobial activity because viscous vehicles release Ca +2 and OH − ions more slowly [5]. Thus, the type and characteristics of materials, such as their superficial microstructure, surface roughness, and fissures, can influence bacterial adhesion and biofilm formation [20]. Despite the differences between our in vitro and in vivo results, it is essential to emphasize that cell culture studies did not characterize the underlying host-response interaction.…”

“…Sealer discs (5 mm in diameter and 2 mm in height) were prepared and kept in an incubator at 37 °C for 72 h. They were then sterilized in ultraviolet light for 1 h. Antimicrobial adhesion was performed as described by Colombo et al [20], with some modifications. Briefly, an inoculum of ~1 × 10 7 (colony forming units/mL) of Enterococcus faecalis (ATCC 29212) was seeded onto each sealer disc and incubated at 37 °C for 2 h. The discs were then washed in phosphate-buffered saline and stained with LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen Molecular Probes, OR, USA) for 10 min.…”

An In-Vitro Analysis of Microleakage of Self-Adhesive Fissure Sealant vs. Conventional and GIC Fissure Sealants

“…There are a few investigations on the adhesive properties of these simplified self-adhesive fissure sealant composite materials [7,8,9]. Some studies reported that SAC achieved lower bond strength compared to the traditional flowable composites that were used with bonding agents [8].…”