Bacterial Adhesion Forces with Substratum Surfaces and the Susceptibility of Biofilms to Antibiotics

Muszanska, Agnieszka K.; Nejadnik, M. Reza; Chen, Yun; Heuvel, Edwin R. van den; Busscher, Henk J.; Mei, Henny C. van der; Norde, Willem

doi:10.1128/aac.00431-12

Cited by 51 publications

(59 citation statements)

References 15 publications

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“…32 Another in vitro study demonstrated that S. aureus, S. epidermidis and P. aeruginosa possess strong forces of adhesion to foreign bodies. 33 In studies of implants from patients having revision for total joint arthroplasty of hip or knee without clinical and routine microbiological evidence of infection, bacterial biofilms including coagulase- negative staphylococci were isolated from the removed implants by using ultrasonication and other sensitive methods, 34,35 indicating a biofilm infection of the implant. Bacteria usually form biofilm on foreign bodies that are placed in patients for medical reasons, such as peripheral and central venous catheters, heart valves, ventricular assisting devices, coronary stents, arthro-prostheses, fracture-fixation devices, breast implants, intraocular lenses, dental implants, etc.…”

Section: Possible Causes Leading To Prosthesis-related Infectionsmentioning

confidence: 99%

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

et al. 2013

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Prosthesis-related infection is a serious complication for patients after orthopedic joint replacement, which is currently difficult to treat with antibiotic therapy. Consequently, in most cases, removal of the infected prosthesis is the only solution to cure the infection. It is, therefore, important to understand the comprehensive interaction between the microbiological situation and the host immune responses that lead to prosthesis infections. Evidence indicates that prosthesis infections are actually biofilm-correlated infections that are highly resistant to antibiotic treatment and the host immune responses. The authors reviewed the related literature in the context of their clinical experience, and discussed the possible etiology and mechanism leading to the infections, especially problems related to bacterial biofilm, and prophylaxis and treatment of infection, including both microbiological and surgical measures. Recent progress in research into bacterial biofilm and possible future treatment options of prosthesis-related infections are discussed.

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Section: Possible Causes Leading To Prosthesis-related Infectionsmentioning

confidence: 99%

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

et al. 2013

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“…Given the similar hydrophilic characters of both alginate lyase and BSA coatings via polydopamine, results suggest that alginate lyase may have another underlying mechanism for preventing bacterial adhesion, beyond preventing nonspecific adhesion. The combination of this preventive approach with therapeutic approaches, namely, antibiotic therapies may hold great potential to fight biomaterial‐associated infections, as it is expected that bacterial cells adhered to these modified surfaces will be more susceptible to antibiotic therapy in a similar way to bacteria that adhered more weakly to brush‐coated silicone rubber, enhancing their susceptibility to gentamicin treatment …”

Section: Resultsmentioning

confidence: 99%

“…The combination of this preventive approach with therapeutic approaches, namely, antibiotic therapies may hold great potential to fi ght biomaterial-associated infections, as it is expected that bacterial cells adhered to these modifi ed surfaces will be more susceptible to antibiotic therapy in a similar way to bacteria that adhered more weakly to brushcoated silicone rubber, enhancing their susceptibility to gentamicin treatment. [ 45 ]…”

Section: Antibacterial Performance Of Surfaces Functionalized With Almentioning

confidence: 99%

Polydopamine‐Mediated Immobilization of Alginate Lyase to Prevent P. aeruginosa Adhesion

Alves

Sileika

Messersmith

2016

Macromolecular Bioscience

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Given alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofilms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is employed for functionalization of polycarbonate surfaces. Enzyme immobilization is confirmed by surface characterization. Surfaces functionalized with alginate lyase exhibit anti-adhesive properties, inhibiting the attachment of the mucoid strain. Moreover, surfaces modified with this enzyme also inhibit the adhesion of the tested non-mucoid strain. Unexpectedly, treatment with heat-inactivated enzyme also inhibits the attachment of mucoid and non-mucoid P. aeruginosa strains. These findings suggest that the antibacterial performance of alginate lyase functional coatings is catalysis-independent, highlighting the importance of further studies to better understand its mechanism of action against P. aeruginosa strains.

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“…Yet it is known that also low numbers of bacteria adhering on a polymer brush coating have the ability to grow over prolonged periods of time into weakly adhering microcolonies. The weak adhesion does not stimulate the transition to a biofilm mode of growth, leaving bacteria potentially in a more antibiotic susceptible state . Our modular coating concept allows to kill these bacteria as soon as they approach the surface.…”

Section: Discussionmentioning

confidence: 99%

“…The weak adhesion does not stimulate the transition to a biofilm mode of growth, leaving bacteria potentially in a more antibiotic susceptible state. [22] Our modular coating concept allows to kill these bacteria as soon as they approach the surface. To this end, the VS-module was complemented with the possibility of sustained CHX release.…”

Section: Discussionmentioning

confidence: 99%

A Trifunctional, Modular Biomaterial Coating: Nonadhesive to Bacteria, Chlorhexidine‐Releasing and Tissue‐Integrating

Sjollema

Keul

Mei

et al. 2016

Macromolecular Bioscience

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Various potential anti-infection strategies can be thought of for biomaterial implants and devices. Permanent, tissue-integrated implants such as artificial joint prostheses require a different anti-infection strategy than, for instance, removable urinary catheters. The different requirements set to biomaterials implants and devices in different clinical applications call for tailor-made strategies. Here, a modular coating-concept for biomaterials is reported, which in its full, trifunctional form comprises nonadhesiveness to bacteria and antimicrobial release, combined with enhanced tissue integration characteristics. Nonadhesiveness to proteins and bacteria is accomplished by a hydrophilic brush coating (Vitrostealth). The antimicrobial release module is constituted by a chlorhexidine releasing poly(ethylene glycol) diacrylamide based-coating that continues to release its antimicrobial content also when underneath the nonadhesive top-coating. The third module, enhancing tissue integration, is realized by the incorporation of the penta-peptide Glycine-Arginine-Glycine-Aspartic acid-Serine (GRGDS) within the nonadhesive top-coating. Modules function in concert or independently of each other. Specifically, tissue integration by the GRGDS-module does not affect the nonadhesiveness of the Vitrostealth-module toward bovine serum albumin and Staphylococcus aureus, while the antimicrobial release module does not affect tissue-integration by the GRGDS-module. Uniquely, using this modular system, tailor-made anti-infection strategies can thus readily be made for biomaterials in different clinical applications.

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Bacterial Adhesion Forces with Substratum Surfaces and the Susceptibility of Biofilms to Antibiotics

Cited by 51 publications

References 15 publications

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

Polydopamine‐Mediated Immobilization of Alginate Lyase to Prevent P. aeruginosa Adhesion

A Trifunctional, Modular Biomaterial Coating: Nonadhesive to Bacteria, Chlorhexidine‐Releasing and Tissue‐Integrating

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