Bacterial acid stress response: from cellular changes to antibiotic tolerance and phenotypic heterogeneity

Schumacher, Kilian; Brameyer, Sophie; Jung, Kirsten

doi:10.1016/j.mib.2023.102367

Cited by 7 publications

(2 citation statements)

References 75 publications

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“…In addition, RND pumps are also important for the survival under this stress as seen from the large delay in the growth of ∆3 cells in pH 4.6 (Figure S3). Efflux appears to be a common adaptation to acidic stress among various bacterial species [28,29]. In E. coli, the inactivation of the RND pump MdtEF-TolC reduces the bacterial fitness in acidic pH and survival in macrophages [30].…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

Leus,

Olvera,

Adamiak

et al. 2023

Antibiotics

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Multidrug efflux transporters are major contributors to the antibiotic resistance of Acinetobacter baumannii in clinical settings. Previous studies showed that these transporters are tightly integrated into the physiology of A. baumannii and have diverse functions. However, for many of the efflux pumps, such functions remain poorly defined. In this study, we characterized two putative drug efflux pumps, AmfAB and AmfCD (Acinetobacter Major Facilitator), that are homologous to EmrAB-like transporters from Escherichia coli and other Gram-negative bacteria. These pumps comprise the Major Facilitator Superfamily (MFS) transporters AmfB and AmfD and the periplasmic membrane fusion proteins AmfA and AmfC, respectively. We inactivated and overproduced these pumps in the wild-type ATCC 17978 strain and its derivative strains lacking the major efflux pumps from the Resistance–Nodulation–Division (RND) superfamily and characterized antibiotic susceptibilities and growth of the strains under stresses typical during human infections. We found that neither AmfAB nor AmfCD contribute to the antibiotic non-susceptibility phenotypes of A. baumannii. The two pumps, however, are critical for the adaptation and growth of the bacterium under acidic stress, whereas AmfCD also contributes to growth under conditions of low iron, high temperature, and in the presence of bile salts. These functions are dependent on the presence of the RND pumps, the inactivation of which further diminishes A. baumannii survival and growth. Our results suggest that MFS transporters contribute to stress survival by affecting the permeability properties of the A. baumannii cell envelope.

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Section: Discussionmentioning

confidence: 99%

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

Leus,

Olvera,

Adamiak

et al. 2023

Antibiotics

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“…In response to the stress of low pH, bacteria have developed some resistance systems (Figure 1). To counteract acidification and survive in a mild and highly acidic environment LAB activate defense mechanisms that regulate homeostasis, maintaining an intracellular pH of about 6.0, thus also contributing to an increase in acid resistance [45]. The main protective mechanism is the removal from the cytoplasm of accumulated protons (H+) by ATPase, which results in an intracellular increase in pH [32].…”

Section: Influence Of Ph On Lab and Their Adaptation To Acid Stressmentioning

confidence: 99%

The Impact of Physicochemical Conditions on Lactic Acid Bacteria Survival in Food Products

Sionek,

Szydłowska,

Trząskowska

et al. 2024

Fermentation

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Lactic acid bacteria (LAB), due to their many advantageous features, have been utilized in food manufacturing for centuries. Spontaneous fermentation, in which LAB play a fundamental role, is one of the oldest methods of food preservation. LAB survival and viability in various food products are of great importance. During technological processes, external physicochemical stressors appear often in combinations. To ensure the survival of LAB, adjustment of optimal physicochemical conditions should be considered. LAB strains should be carefully selected for particular food matrices and the technological processes involved. The LAB’s robustness to different environmental stressors includes different defense mechanisms against stress, including the phenomenon of adaptation, and cross-protection. Recently established positive health effects and influence on human wellbeing have caused LAB to be some of the most desirable microorganisms in the food industry. A good understanding of LAB defense and adaptation mechanisms can lead to both optimization of food production and storage conditions, as well as to obtaining LAB strains with increased tolerance to stressors. Hopefully, as a result, the final food product with naturally present or added LAB can achieve outstanding quality and safety with health benefits that meet consumer expectations.

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Catalyst-free regeneration of plasma-activated water via ultrasonic cavitation: Removing aggregation concealment of antibiotic-resistant bacteria with enhanced wastewater sustainability

Hu,

Xu,

Cheng

et al. 2024

Journal of Hazardous Materials

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Bacterial acid stress response: from cellular changes to antibiotic tolerance and phenotypic heterogeneity

Cited by 7 publications

References 75 publications

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

The Impact of Physicochemical Conditions on Lactic Acid Bacteria Survival in Food Products

Catalyst-free regeneration of plasma-activated water via ultrasonic cavitation: Removing aggregation concealment of antibiotic-resistant bacteria with enhanced wastewater sustainability

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