Bacteria-targeted biomaterials: Glycan-coated microspheres to bind Helicobacter pylori

Gonçalves, Inês C.; Magalhães, Ana; Costa, Ana Margarida; Oliveira, José; Henriques, Patrícia C.; Gomes, Paula; Reis, Celso A.; Martins, M. Cristina L.

doi:10.1016/j.actbio.2016.01.029

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…Afterwards, we demonstrated that these ChMics are not cytotoxic and can adhere several strains of H. pylori under different pH [56]. Lastly, these ChMics were modified with glycans to make them H. pylori-strain specific, being able to prevent/remove H. pylori adhesion to gastric mucosa expressing the same glycans (in vitro and ex vivo) [57]. These findings reveal the potential of these ChMics as an antibiotic-free alternative system to fight gastric infection.…”

Section: Introductionmentioning

confidence: 74%

“…The high voltage electrostatic system, previously used to produce microspheres with 170 μm diameter [49,56,57], allows only the production of particles with sizes between 100-350 μm, therefore not as small as desired. The herein described aerodynamically driven system allows the production of a wider range of particle sizes (from 20 to 400 μm), being 75 times faster (mL/min comparing to mL/h), a relevant factor when envisaging a large-scale production.…”

Section: Chmics Characterizationmentioning

confidence: 99%

“…pylori presence in the stomach could be potentially improved through exploitation of several approaches. Specifically regarding the treatment duration, for instance, although the 2 weeks treatment applied followed the guidelines of the currently used antibiotic therapy [5] [57]. High specificity and in vivo anti-H. pylori efficiency was observed when urea or fucose were incorporated in nanoparticles [30,32], as reported for pH-sensitive amoxicillin-loaded ureido-conjugated chitosan derivatives (UCCs)-conjugated poly(lactic-co-glycolic acid)…”

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confidence: 97%

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Orally administrated chitosan microspheres bind Helicobacter pylori and decrease gastric infection in mice

et al. 2020

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Section: Introductionmentioning

confidence: 74%

Section: Chmics Characterizationmentioning

confidence: 99%

mentioning

confidence: 97%

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Orally administrated chitosan microspheres bind Helicobacter pylori and decrease gastric infection in mice

et al. 2020

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“…Based on the critical role that glycans play in the adhesion of H. pylori to the gastric epithelium and the establishment of a chronic infection, different strategies aiming the blockage of the glycan-mediated adhesion have been proposed. These alternative treatment strategies include the use of glycan-decorated chitosan microspheres, which were shown to prevent and remove H. pylori binding to gastric cell lines and to gastric mucosal tissue sections [ 66 , 67 ].…”

Section: O- Glycosylation Alterations During Gamentioning

confidence: 99%

Mucin-Type O-Glycosylation in Gastric Carcinogenesis

et al. 2016

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Mucin-type O-glycosylation plays a crucial role in several physiological and pathological processes of the gastric tissue. Modifications in enzymes responsible for key glycosylation steps and the consequent abnormal biosynthesis and expression of their glycan products constitute well-established molecular hallmarks of disease state. This review addresses the major role played by mucins and associated O-glycan structures in Helicobacter pylori adhesion to the gastric mucosa and the subsequent establishment of a chronic infection, with concomitant drastic alterations of the gastric epithelium glycophenotype. Furthermore, alterations of mucin expression pattern and glycan signatures occurring in preneoplastic lesions and in gastric carcinoma are also described, as well as their impact throughout the gastric carcinogenesis cascade and in cancer progression. Altogether, mucin-type O-glycosylation alterations may represent promising biomarkers with potential screening and prognostic applications, as well as predictors of cancer patients’ response to therapy.

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“…SAMs are easy to prepare, functionalize and control at molecular scale, being compatible with many surface characterization techniques used for AMP grafting detection 26,27 . Furthermore, SAMs have been previously used by us to demonstrate the specific recognition between H. pylori adhesins (BabA and SabA) and surface grafted glycans (Lewis b and sialyl-Lewis x) 28 , being afterwards this knowledge translated onto biocompatible polymers (chitosan microspheres) 29 . AMPs described in the literature as active against H. pylori were selected, namely: Odonorrain-HP 20 , MSI-78 (pexiganan) 25 and MSI-78A 21–23 , which is derived from the MSI-78 by replacement of one amino acid (G13A).…”

Section: Introductionmentioning

confidence: 99%

Surface Grafted MSI-78A Antimicrobial Peptide has High Potential for Gastric Infection Management

Parreira

Monteiro

Graça

et al. 2019

Sci Rep

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As we approach the end of the antibiotic era, newer therapeutic options, such as antimicrobial peptides (AMPs), are in urgent demand. AMP surface grafting onto biomaterials has been described as a good strategy to overcome problems associated with their in vivo stability. Helicobacter pylori is among the bacteria that pose greatest threat to human health, being MSI-78A one of the few bactericidal AMPs against this bacterium. Here, we report that MSI-78A grafted onto model surfaces (Self-Assembled Monolayers –SAMs), in a concentration of 30.3 ± 1.2 ng/cm2 determined by quartz crystal microbalance with dissipation (QCM-D), was able to kill, by contact, 98% of planktonic H. pylori in only 2 h. This fact was not verified against the control bacteria (Staphylococcus epidermidis), although the minimal inhibitory concentration (MIC) of MSI-78A in solution is much lower for S. epidermidis (2 μg/mL) than for H. pylori (64 μg/mL). Our results also demonstrated that, in opposite to other bacteria, H. pylori cells were attracted to ethylene glycol terminated (antiadhesive) surfaces, which can explain the high bactericidal potential of grafted MSI-78A. This proof of concept study establishes the foundations for development of MSI-78A grafted nanoparticles for gastric infection management within a targeted nanomedicine concept.

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Bacteria-targeted biomaterials: Glycan-coated microspheres to bind Helicobacter pylori

Cited by 16 publications

References 32 publications

Orally administrated chitosan microspheres bind Helicobacter pylori and decrease gastric infection in mice

Orally administrated chitosan microspheres bind Helicobacter pylori and decrease gastric infection in mice

Mucin-Type O-Glycosylation in Gastric Carcinogenesis

Surface Grafted MSI-78A Antimicrobial Peptide has High Potential for Gastric Infection Management

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