Bacteria-released outer membrane vesicles promote disseminated intravascular coagulation

Wang, Erhua; Liu, Yukun; Qiu, Xianhui; Tang, Yiting; Wang, Huadong; Xiao, Xianzhong; Chen, Fangping; Lü, Ben

doi:10.1016/j.thromres.2019.03.019

Cited by 25 publications

(19 citation statements)

References 27 publications

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“…OMVs can also trigger mitochondrial apoptosis and the inflammasome pathway in macrophages and dendritic cells ( 38 , 39 , 49 , 50 ). And finally, OMVs have been shown, in mice, to induce disseminated intravascular coagulation, a severe complication of sepsis that significantly increases the probability of mortality in septic patients ( 51 , 52 ).…”

Section: Evs As Molecular Biomarkers For Sepsismentioning

confidence: 99%

Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature’s perfect packages

Michel

Gaborski²

2022

Journal of Biological Chemistry

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Section: Evs As Molecular Biomarkers For Sepsismentioning

confidence: 99%

Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature’s perfect packages

Michel

Gaborski²

2022

Journal of Biological Chemistry

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“…There is currently no history, physical examination, or laboratory component that can accurately diagnose or rule out DIC in a patient. Both gram-negative and gram-positive bacteria may cause DIC, although it is more common in gram-negative bacteria [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Disseminated Intravascular Coagulation in Sepsis and Associated Factors

Rinaldi

Sudaryo

Prihartono

2022

JCM

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Background: sepsis is a life-threatening organ dysfunction caused by an excessive host immunological response to infection. The incidence of sepsis is increasing every year, and sepsis is the primary cause of mortality in intensive care units (ICUs). DIC is a coagulopathy syndrome that causes microvascular and macrovascular thrombosis and increases the risk of bleeding due to consumptive coagulopathy. The pathophysiology of DIC in sepsis is complex, and further research is required to investigate the involved mechanisms and risk factors. Method: this study is a prognostic analysis of a retrospective cohort. Samples were patients diagnosed with sepsis and admitted to Cipto Mangunkusumo National General Hospital from January 2016 to October 2022. Research subjects were followed until occurrence of DIC during sepsis or recovery from sepsis. The research subjects were selected from medical records using a consecutive total sampling approach. The inclusion criteria were patients aged ≥18 years old and diagnosed with sepsis according to qSOFA criteria with a score of 2. The exclusion criterion was an incomplete medical record. Bivariate and multivariate logistic regression analyses were performed to determine which independent variables contributed to the incidence of DIC and obtain the odds ratios (ORs). p < 0.05 was considered to indicate a statistically significant difference. Results: a total of 248 patients were included after considering the inclusion and exclusion criteria. Of these, 50 (20.2%) septic patients developed DIC. In the multivariate analysis, albumin ≤2.5 g/dL (OR: 2.363; 95% CI: 1.201–4.649), respiratory infection (OR: 2.414; 95% CI: 1.046–5.571), and antibiotic treatment ≥1 h (OR: 2.181; 95% CI: 1.014–4.689) were associated with DIC development. On the basis of the ROC curve, the area under the curve (AUC) was determined to be 0.705 with 95% CI = (0.631–0.778). Conclusion: in our study, the prevalence of DIC in septic patients was 20.2%. Low albumin, respiratory infection, and antibiotic treatment ≥1 h were found to be risk factors for development of DIC in septic patients.

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“…The release of membrane vesicles is an ubiquitous process and was observed among a wide range of bacteria. Not only pathogenic bacteria such as for example Vibrio cholerae, Campylobacter jejuni, Helicobacter pylori, Aggregatibacter actinomycetemcomitans, Pseudomonas aeruginosa, Moraxella catarrhalis, Stenotrophomonas maltophilia, Acinetobacter baumannii, Shigella flexneri, Salmonella enterica serovar Typhimurium, enterotoxigenic Escherichia coli (ETEC), enterohemorrhagic E. coli (EHEC), adherent-invasive E. coli, and extraintestinal pathogenic E. coli, but also non-pathogenic bacteria such as E. coli Nissle 1917, shed membrane vesicles during growth Beveridge, 1995, 1997;Wai et al, 1995Wai et al, , 2003Kuehn, 2000, 2002;Balsalobre et al, 2006;Kouokam et al, 2006;Bomberger et al, 2009;Lindmark et al, 2009;Ellis and Kuehn, 2010;Rolhion et al, 2010;Chatterjee and Chaudhuri, 2011;Rumbo et al, 2011;Schaar et al, 2011;Rompikuntal et al, 2012Rompikuntal et al, , 2015Guidi et al, 2013;Kunsmann et al, 2015;Elhenawy et al, 2016;Bielaszewska et al, 2017;Chatterjee et al, 2017;Devos et al, 2017;Fabrega et al, 2017;Svennerholm et al, 2017;Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Defensively, they can be used to sequester antibiotics, bacteriophages, and antibodies, bind or degrade antimicrobial peptides, as well as bait antigens to distract the immune system (Manning and Kuehn, 2011;MacDonald and Kuehn, 2012;Duperthuy et al, 2013;O'Donoghue and Krachler, 2016;Urashima et al, 2017;Reyes-Robles et al, 2018). The potential of OMVs as offensive weapons is evident in their ability to deliver virulence factors into host cells ( Figure 1BIII) Tan et al, 2007;Bomberger et al, 2009;Amano et al, 2010;Ellis and Kuehn, 2010;Kulp and Kuehn, 2010;Schaar et al, 2011;Rompikuntal et al, 2012;Bielaszewska et al, 2013Bielaszewska et al, , 2017Kunsmann et al, 2015;O'Donoghue and Krachler, 2016;Rüter et al, 2018) as well as to induce sepsis, sepsis-associated cardiomyopathy or disseminated intravascular coagulation in the absence of intact bacterial cells (Park et al, 2010;Shah et al, 2012;Svennerholm et al, 2017;Wang et al, 2019). The OMVassociated LPS does not only appear to be effective through the extracellular Toll-like receptor (TLR) 4 (Kunsmann et al, 2015;Bielaszewska et al, 2018;Wang et al, 2019) or TLR2 (Schaar et al, 2011), as OMV-bound LPS can also activate the non-canonical inflammasome signaling pathway intracellularly after uptake of the OMVs into the target cells (Vanaja et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The potential of OMVs as offensive weapons is evident in their ability to deliver virulence factors into host cells ( Figure 1BIII) Tan et al, 2007;Bomberger et al, 2009;Amano et al, 2010;Ellis and Kuehn, 2010;Kulp and Kuehn, 2010;Schaar et al, 2011;Rompikuntal et al, 2012;Bielaszewska et al, 2013Bielaszewska et al, , 2017Kunsmann et al, 2015;O'Donoghue and Krachler, 2016;Rüter et al, 2018) as well as to induce sepsis, sepsis-associated cardiomyopathy or disseminated intravascular coagulation in the absence of intact bacterial cells (Park et al, 2010;Shah et al, 2012;Svennerholm et al, 2017;Wang et al, 2019). The OMVassociated LPS does not only appear to be effective through the extracellular Toll-like receptor (TLR) 4 (Kunsmann et al, 2015;Bielaszewska et al, 2018;Wang et al, 2019) or TLR2 (Schaar et al, 2011), as OMV-bound LPS can also activate the non-canonical inflammasome signaling pathway intracellularly after uptake of the OMVs into the target cells (Vanaja et al, 2016). The uptake of OMV occurs either by phagocytosis or by classic endocytosis Bielaszewska et al, 2013Bielaszewska et al, , 2017Rewatkar et al, 2015;O'Donoghue and Krachler, 2016), whereupon the virulence factors differentially separate during the intracellular transport of the OMVs and can develop their toxic activities (Bielaszewska et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Secretion and Delivery of Intestinal Pathogenic Escherichia coli Virulence Factors via Outer Membrane Vesicles

Rueter

Bielaszewska

2020

Front. Cell. Infect. Microbiol.

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Outer membrane vesicles (OMVs) are nanoscale proteoliposomes secreted from the cell envelope of all Gram-negative bacteria. Originally considered as an artifact of the cell wall, OMVs are now recognized as a general secretion system, which serves to improve the fitness of bacteria and facilitate bacterial interactions in polymicrobial communities as well as interactions between the microbe and the host. In general, OMVs are released in increased amounts from pathogenic bacteria and have been found to harbor much of the contents of the parental bacterium. They mainly encompass components of the outer membrane and the periplasm including various virulence factors such as toxins, adhesins, and immunomodulatory molecules. Numerous studies have clearly shown that the delivery of toxins and other virulence factors via OMVs essentially influences their interactions with host cells. Here, we review the OMV-mediated intracellular deployment of toxins and other virulence factors with a special focus on intestinal pathogenic Escherichia coli. Especially, OMVs ubiquitously produced and secreted by enterohemorrhagic E. coli (EHEC) appear as a highly advanced mechanism for secretion and simultaneous, coordinated and direct delivery of bacterial virulence factors into host cells. OMV-associated virulence factors are not only stabilized by the association with OMVs, but can also often target previously unknown target structures and perform novel activities. The toxins are released by OMVs in their active forms and are transported via cell sorting processes to their specific cell compartments, where they can develop their detrimental effects. OMVs can be considered as bacterial "long distance weapons" that attack host tissues and help bacterial pathogens to establish the colonization of their biological niche(s), impair host cell function, and modulate the defense of the host. Thus, OMVs contribute significantly to the virulence of the pathogenic bacteria.

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Bacteria-released outer membrane vesicles promote disseminated intravascular coagulation

Cited by 25 publications

References 27 publications

Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature’s perfect packages

Outer membrane vesicles as molecular biomarkers for Gram-negative sepsis: Taking advantage of nature’s perfect packages

Disseminated Intravascular Coagulation in Sepsis and Associated Factors

Secretion and Delivery of Intestinal Pathogenic Escherichia coli Virulence Factors via Outer Membrane Vesicles

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