Bacteria-material surface interactions: methodological development for the assessment of implant surface induced antibacterial effects

Zaborowska, Magdalena; Welch, Ken; Brånemark, Rickard; Khalilpour, Poroshat; Engqvist, Håkan; Thomsen, Peter; Trobos, Margarita

doi:10.1002/jbm.b.33179

Cited by 37 publications

(28 citation statements)

References 49 publications

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“…It is easy to distinguish between technologies offering anti-adhesive properties , those working as antimicrobial agents and those combining the above-mentioned approaches . Anti-infective surfaces can be classified as “ contact killing” and antimicrobial agent eluting respectively [150]. …”

Section: General Remarks On Prosthetic Implant Surface Modificationsmentioning

confidence: 99%

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Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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Prosthetic joint infection (PJI) is a feared complication of total joint arthroplasty associated with increased morbidity and mortality. There is a growing body of evidence that bacterial colonization and biofilm formation are critical pathogenic events in PJI. Thus, the choice of biomaterials for implanted prostheses and their surface modifications may significantly influence the development of PJI. Currently, silver nanoparticle (AgNP) technology is receiving much interest in the field of orthopaedics for its antimicrobial properties and a strong anti-biofilm potential. The great advantage of AgNP surface modification is a minimal release of active substances into the surrounding tissue and a long period of effectiveness. As a result, a controlled release of AgNPs could ensure antibacterial protection throughout the life of the implant. Moreover, the antibacterial effect of AgNPs may be strengthened in combination with conventional antibiotics and other antimicrobial agents. Here, our main attention is devoted to general guidelines for the design of antibacterial biomaterials protected by AgNPs, its benefits, side effects and future perspectives in PJI prevention.

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Section: General Remarks On Prosthetic Implant Surface Modificationsmentioning

confidence: 99%

“…, total joint arthroplasty, internal, external fixation) should be further developed and appropriately validated. Given the large variability of antibacterial strategies, it is likely that testing methods must be better tailored to match the specific proposed strategy at hand [150]. …”

Section: Protocol For Testing Of Silver Nanoparticle Coating Techmentioning

confidence: 99%

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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“…Various methods for the quantification of bacterial colonizations are known from literature and a “gold standard” does not exist, since each method will give an answer to a specific question [14]. The specific application of the material has to be considered when choosing the right cultivation and quantification method [18,21].…”

Section: Discussionmentioning

confidence: 99%

“…Many analytical techniques to study and quantify microbial colonization on abiotic surfaces are presently in use [13,14]. Depending on the kind of material, its mode of action and possible application site, different methods and experimental setups for investigation of the susceptibility to bacterial colonization might be required.…”

Section: Introductionmentioning

confidence: 99%

Flow Chamber System for the Statistical Evaluation of Bacterial Colonization on Materials

et al. 2016

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Biofilm formation on materials leads to high costs in industrial processes, as well as in medical applications. This fact has stimulated interest in the development of new materials with improved surfaces to reduce bacterial colonization. Standardized tests relying on statistical evidence are indispensable to evaluate the quality and safety of these new materials. We describe here a flow chamber system for biofilm cultivation under controlled conditions with a total capacity for testing up to 32 samples in parallel. In order to quantify the surface colonization, bacterial cells were DAPI (4`,6-diamidino-2-phenylindole)-stained and examined with epifluorescence microscopy. More than 100 images of each sample were automatically taken and the surface coverage was estimated using the free open source software g’mic, followed by a precise statistical evaluation. Overview images of all gathered pictures were generated to dissect the colonization characteristics of the selected model organism Escherichia coli W3310 on different materials (glass and implant steel). With our approach, differences in bacterial colonization on different materials can be quantified in a statistically validated manner. This reliable test procedure will support the design of improved materials for medical, industrial, and environmental (subaquatic or subaerial) applications.

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“…The materials were inoculated with a dose of 10 5 CFU by dilution of the OD in TSB. The cells were seeded on either PU catheter segments in glass tubes or on Ti discs in 48‐well plates (Nunclon Delta‐surface; Thermo Fisher Scientific, Denmark) and incubated at 37°C under shaking (150 rpm) or static conditions, respectively, for 4–96 h. The viable cell population of surface‐adherent bacterial cells was quantified by colony‐forming unit (CFU) counting as described previously . For SEM imaging, biofilms on Ti and PU [Fig.…”

Section: Methodsmentioning

confidence: 99%

Staphylococcal biofilm gene expression on biomaterials — A methodological study

Juhlin

Svensson

Thomsen

et al. 2017

J Biomedical Materials Res

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The combination of increased healthcare access, universal aging, and infallible therapy demands, synergistically drive the need for the development of biomaterial technologies that mitigate the challenge of biomaterial-associated infections (BAI). Staphylococcus epidermidis and Staphylococcus aureus account for the majority of BAI due to their ability to accumulate in adherent multilayered biofilm. This investigation details the development of gene expression assays to evaluate the genetic processes of attachment, accumulation, maturation, and dispersal phases of biofilms on biomaterials in vitro, while abiding by the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. The biofilm formation of S. epidermidis on polyurethane (PU) central venous catheters and S. aureus on machined titanium (Ti) was examined in terms of gene expression at early and late time points. The results provided insight into how each stage of biofilm formation is orchestrated over time on these biomaterials in vitro. Furthermore, the results suggested that mechanical RNA extraction, organic solvents, elimination of genomic DNA, and preamplification are advisable strategies to implement for biofilm gene expression analysis. It is concluded that this method can be employed for the assessment of biofilm-biomaterial interactions at the molecular level. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3400-3412, 2017.

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Bacteria-material surface interactions: methodological development for the assessment of implant surface induced antibacterial effects

Cited by 37 publications

References 49 publications

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

Flow Chamber System for the Statistical Evaluation of Bacterial Colonization on Materials

Staphylococcal biofilm gene expression on biomaterials — A methodological study

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