Bacteria Isolated from the Aeration Chamber of Wastewater Treatment Plants Used in the Biocontrol and Promotion of Wheat Growth

Abstract

Background: Antagonisms against Fusarium spp. and multi-traits to protect and improve fertilization of wheat by bacterial strains from activated sludge were assessed. Methods: Isolated strains obtained were identified by 16S rRNA gene sequencing and the MALDI-TOF method, and their enzymatic profile was investigated. Treated plant growth-promoting bacteria (PGPB) wheat kernels were grown in pots with soil contaminated with Fusarium conidia. Activated sludge is a collection of microorganisms exposed to strong environmental pressure (chemicals) and antagonistic properties. Results: The isolated bacterial strains were similar to: Ps-1 (Serratia liquefaciens), Ps-15 (Serratia sp.) and Ps-9 (Pseudomonas helleri). The dual culture assay showed the highest antagonism of Ps-9 vs. Fusarium spp. The tested bacteria showed activity in the production of chitinase, a variety of proteases, enzymes that degrade various sugars, and esterase, which creates a complex that allows for a variety of strategies to control phytopathogens. The Ps-9 strain was able to solubilize phosphate. The Ps-9 and Ps-15 strains showed good ammonification ability. A marked improvement was observed in test variants in pots inoculated with Fusarium spores after the use of Ps-9. The Ps-9 strain reduced the disease index to traces of symptoms of both species of Fusarium and increased the grain weight. Conclusions: The Ps-9 strain was proven to have high potential for application in the biocontrol and promotion of wheat growth.

Modulation of drought adversities in Vicia faba by the application of plant growth promoting rhizobacteria and biochar

“…IAA is the main auxin in plants, which regulates the growth and developmental processes of plants including cell division, cell elongation, apical dominance, tissue differentiation and responses to various environmental stressors (Yousef, 2018). Alkaline phosphatase undergo the growth governance and catalyze the release of phosphate esters (Thangam & Rajkumar, 2002), valine arylamidase catalyze the nitrogen metabolism in plants (Przemieniecki et al, 2020),…”

“…IAA is the main auxin in plants, which regulates the growth and developmental processes of plants including cell division, cell elongation, apical dominance, tissue differentiation and responses to various environmental stressors (Yousef, 2018). Alkaline phosphatase undergo the growth governance and catalyze the release of phosphate esters (Thangam & Rajkumar, 2002), valine arylamidase catalyze the nitrogen metabolism in plants (Przemieniecki et al, 2020), Napthol‐As‐BI‐phosphohydrolase undergoes defense of the plant from biotic as well as abiotic stressors (Sampaio et al, 2017), and esterase lipase are involved in the regulation and morphogenesis of plant development, defense responses and synthesis of secondary metabolites (Chepyshko et al, 2012). The aforementioned studies confirm the plant growth promoting nature of both bacterial strains and may be helpful for the mitigation of drought stress in V. faba plant.…”

Insect Gut Bacteria Promoting the Growth of Tomato Plants (Solanum lycopersicum L.)

“…Many PGPB belong to well-known families like Enterobacteriaceae , Pseudomonaceae, or Bacillaceae , members of which have been described previously as plant growth promoting bacteria. In addition, PGPB inhabits ecological niches other than the rhizosphere and plant surface [ 16 ], and multiple plant-insect-bacteria interactions were described [ 36 ], including the role of rhizobacteria in the control of pest insects in agriculture [ 37 ]. That is why, knowing that some species within the families are closely related and have similar ecology, we decided to test if: (i) bacteria other than directly plant-associated exhibit PGP properties, and (ii) whether such bacteria can be used as ingredients of PGP bacterial (or microbial) consortia, that potentially after the formulation process could be used as biopreparates in plant production.…”

“…That is why, in general, as PGPB, we consider only the bacteria directly connected with plants, e.g., endophytic or rhizospheric. However, plant growth-promoting bacteria are present in niches other than those directly connected with plants like the rhizosphere or plant surface [ 16 ]. In fact, in nature, there is a whole continuum of bacteria that interact with plants, e.g., through the plant-insect relationship.…”

First organic fluorescence immunoassay for the detection of Enterobacter cloacae in food matrixes

“…13 The World Health Organization has recommended the permissible limit of E. cloacae as 100 CFU g −1 . 14,15 Moreover, when the limit is exceeded, these pathogens cause serious health issues such as respiratory tract infection, urinary system infection, and skin infection in both humans and animals. 16 Therefore, the effective detection of E. cloacae is considered an urgent need because of its pathogenic nature towards humans and animals.…”