Bacteria isolated from hospital, municipal and slaughterhouse wastewaters show characteristic, different resistance profiles

Sib, Esther; Lenz-Plet, Franziska; Barabasch, Vanessa; Klanke, Ursula; Savin, Mykhailo; Hembach, Norman; Schallenberg, Anna; Kehl, Katja; Albert, Cathrin; Gajdiss, Mike; Zacharias, Nicole; Müller, Heike; Schmithausen, Ricarda Maria; Exner, Martin; Kreyenschmidt, Judith; Schreiber, Christiane; Schwartz, Thomas; Parčina, Marijo; Bierbaum, Gabriele

doi:10.1016/j.scitotenv.2020.140894

Cited by 29 publications

(12 citation statements)

References 73 publications

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“…2022; Sib et al . 2020). Such high concentrations that are in the range of MICs and MSCs also explain the difficulties that are encountered, when elimination of these organisms from hospital drains is attempted (Kizny Gordon et al .…”

Section: Discussionmentioning

confidence: 99%

“…The antibiotic concentration measurements and MSCs indicate that high antibiotic concentrations, especially of ciprofloxacin and concentration peaks of β‐lactams, may be the drivers in the selection of multi‐resistant bacteria in this biotope (Sib et al . 2020; Kehl et al . 2022) and must be taken into account, when remediation measures are planned.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, we had shown that a surprisingly high diversity of bacterial strains (belonging to the orders Enterobacterales and Pseudomonadales) with simultaneous resistance to ciprofloxacin, carbapenems, 3 rd generation cephalosporins, and piperacillin/ tazobactam was present in the wastewater of a maximum care hospital (Kehl et al 2022;Sib et al 2020). Such bacteria were also found repeatedly in the biofilms of the drains in the patients rooms (Kehl et al 2022;Sib et al 2019).…”

Section: Introductionmentioning

confidence: 96%

“…2022; Sib et al . 2020). Such bacteria were also found repeatedly in the biofilms of the drains in the patients rooms (Kehl et al .…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic concentrations in raw hospital wastewater surpass minimal selective and minimum inhibitory concentrations of resistant Acinetobacter baylyi strains

Schuster

Holstein

Axtmann

et al. 2022

Environmental Microbiology

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Antibiotics are essential for modern medicine, they are employed frequently in hospitals and, therefore, present in hospital wastewater. Even in concentrations, that are lower than the minimum inhibitory concentrations (MICs) of susceptible bacteria, antibiotics may exert an influence and select resistant bacteria, if they exceed the MSCs (minimal selective concentrations) of resistant strains. Here, we compare the MSCs of fluorescently labelled Acinetobacter baylyi strains harboring spontaneous resistance mutations or a resistance plasmid with antibiotic concentrations determined in hospital wastewater. Low MSCs in the μg/L range were measured for the quinolone ciprofloxacin (17 μg/L) and for the carbapenem meropenem (30 μg/L). A 24 h continuous analysis of hospital wastewater showed daily fluctuations of the concentrations of these antibiotics with distinctive peaks at 7-8 p. m. and 5-6 a.m. The meropenem concentrations were always above the MSC and MIC values of A. baylyi. In addition, the ciprofloxacin concentrations were in the range of the lowest MSC for about half the time. These results explain the abundance of strains with meropenem and ciprofloxacin resistance in hospital wastewater and drains.Originality-Significance Statement: In previous studies, we had isolated a great variety of multi-resistant bacteria (simultaneously resistant to the critically important antibiotics 3 rd gen. cephalosporins, carbapenems, piperacillin/tazobactam, and ciprofloxacin) from the drains and the wastewater of a maximum care hospital. These bacteria reach the environment, since they are still detected in the effluent of the wastewater treatment plant and they cause infections that are very difficult to treat. So far, it has been assumed that good hygienic measures should be able to avoid nosocomial infections of patients with such bacteria. However, the key aspect of this manuscript is that fluctuating high antibiotic concentrations of meropenem, ciprofloxacin and piperacillin are present in hospital wastewater and that these antibiotic concentrations reach or surpass the minimal selective concentrations of the model organism Acinetobacter baylyi. In conclusion, the colonization of hospital drains and wastewater pipes with multi-resistant bacteria is probably driven by the high antibiotic concentrations in the wastewater. These results are very important, since the antibiotic concentrations and the resulting selective pressure will complicate attempts to such remove the bacteria from the drains and wastewater pipes by cleaning or disinfection measures.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

“…2022; Sib et al . 2020). Such bacteria were also found repeatedly in the biofilms of the drains in the patients rooms (Kehl et al .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antibiotic concentrations in raw hospital wastewater surpass minimal selective and minimum inhibitory concentrations of resistant Acinetobacter baylyi strains

Schuster

Holstein

Axtmann

et al. 2022

Environmental Microbiology

Self Cite

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“…Wastewater emitted by hospitals contain high concentrations of pharmaceutical compounds (Verlicchi et al 2010b), pathogens, (multi-)resistant bacteria and their resistance genes (Schwartz & Alexander 2014;Sib et al 2020). As concentrations of the aforementioned components in hospital wastewater exceed the respective concentrations in municipal wastewater (Verlicchi et al 2010b;Chonova et al 2016;Dinh et al 2017;Exner et al 2018;Paulus et al 2019;Verburg et al 2019), hospitals can be classified as 'hot-spots' (Schwartz & Alexander 2014;Hocquet et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Hospital wastewater treatment and the role of membrane filtration – removal of micropollutants and pathogens: A review

Klatt

Beyer

Einfeldt

2022

Water Science and Technology

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Dissemination of multiresistant bacteria and high concentrations of micropollutants by hospitals and other medical facilities can be significantly reduced by a wide variety of on-site treatment approaches. Membrane filtration technologies, ranging from microfiltration to reverse osmosis, have been adapted in many studies and offer multiple purposes in advanced wastewater treatment configurations. While the direct rejection of pharmaceutical compounds and pathogens can only be achieved with nanofiltration and reverse osmosis processes, porous membranes are known for their pathogen removal capabilities and can be used in combination with other advanced treatment approaches, such as oxidation and adsorption processes. This review was conducted to systematically assess studies with membrane filtration technologies that are used as either stand-alone or hybrid systems for the treatment of hospital wastewater. In this review, four different databases were screened with a pre-set of search strings to thoroughly investigate the application of membrane filtration technology in hospital wastewater treatment. Hybrid systems that combine multiple treatment technologies seem to be the most promising way of consistently removing micropollutants and pathogens from hospital wastewater, but additional economic assessments are needed for an extensive evaluation.

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Health risks from crop irrigation with treated wastewater containing antibiotic residues, resistance genes, and resistant microorganisms

Smalla

Kabisch

Fiedler

et al. 2023

Bundesgesundheitsbl

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ZusammenfassungDiese Übersichtsarbeit beschreibt Effekte und mögliche Gesundheitsrisiken durch resistente Mikroorganismen, Resistenzgene und Biozid- und Arzneimittelrückstände, die durch die Nutzung von aufbereitetem Abwasser zur Bewässerung von Nutzpflanzen entstehen können. Dabei fokussiert die Arbeit auf spezifische Aspekte dieser Kontaminanten und ihrer Interaktionen, strebt jedoch keine allgemeine Bewertung der mikrobiologischen Belastungen an.In aufbereitetem Abwasser werden regelmäßig Rückstände von antibiotisch wirksamen Arzneimitteln, resistente Mikroorganismen und Resistenzgene nachgewiesen. Diese beeinflussen das Boden- und Pflanzenmikrobiom und können von den Pflanzen aufgenommen werden. Mit einer Interaktion von Antibiotikarückständen und Mikroorganismen ist vor allem vor der Ausbringung der aufbereiteten Abwässer zu rechnen. Sie kann allerdings auch das Pflanzenmikrobiom beeinflussen, inklusive der Gesamtheit seiner Resistenzgene (Resistom). Eine besondere Problematik ergibt sich dadurch, dass Pflanzen als Lebensmittel häufig roh, also ohne keimreduzierende Verarbeitungsschritte verzehrt werden. Gründliches Waschen pflanzlicher Lebensmittel beeinflusst das Pflanzenmikrobiom nur geringfügig. Zerkleinerungsschritte bei der Verarbeitung können hingegen zur Vermehrung vorhandener Mikroorganismen beitragen, so dass danach eine gründliche Kühlung erforderlich ist.Bei der Aufbereitung von Abwässern, die zur Bewässerung von Nutzpflanzen eingesetzt werden sollen, werden neue Verfahren zur Elimination von Mikroschadstoffen und Mikroorganismen benötigt, um das Risiko einer verstärkten Exposition von Verbraucherinnen und Verbrauchern gegenüber übertragbaren Resistenzgenen und resistenten Bakterien weiter zu reduzieren.

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Bacteria isolated from hospital, municipal and slaughterhouse wastewaters show characteristic, different resistance profiles

Cited by 29 publications

References 73 publications

Antibiotic concentrations in raw hospital wastewater surpass minimal selective and minimum inhibitory concentrations of resistant Acinetobacter baylyi strains

Antibiotic concentrations in raw hospital wastewater surpass minimal selective and minimum inhibitory concentrations of resistant Acinetobacter baylyi strains

Hospital wastewater treatment and the role of membrane filtration – removal of micropollutants and pathogens: A review

Health risks from crop irrigation with treated wastewater containing antibiotic residues, resistance genes, and resistant microorganisms

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