Bacteria isolated after unsuccessful endodontic treatment in a North American population

Hancock, H.H.; Sigurdsson, Asgeir; Trope, Martin; Moiseiwitsch, Julian

doi:10.1067/moe.2001.113587

Cited by 357 publications

(293 citation statements)

References 24 publications

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“…This bacteria has been identifi ed as the most commonly recovered species from teeth with persistent endodontic infections [5,6], especially when the bacteria are organized in biofi lms attached to the canal walls or located in isthmuses and ramifi cations where they are defi nitely more diffi cult to eliminate. Bovine dentine specimens were selected because they are a satisfactory substratum for bacterial adhesion and biofi lm formation.…”

Section: Discussionmentioning

confidence: 99%

“…Anaerobic bacteria, especially Gram-negative species of Prevotella and Porphyromonas, are frequently associated with signs and symptoms of endodontic origin [2][3][4], and facultative species such as Enterococcus faecalis have been isolated in high frequency from refractory cases [5]. E. faecalis is rarely observed in cases of primary infections, but has been found to be the major component of microfl ora in cases of retreatment, amounting to as much as 70% of the microbiota isolated in these cases [6].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Martos¹,

Ferrer-Luque²,

González-Rodrı́guez³

et al. 2013

European Journal of Microbiology and Immunology

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The Enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. The antimicrobial activity of essential oils and chloroform (CHL), alone and in association with various concentrations of cetrimide (CTR), against biofilm of Enterococcus faecalis was investigated. Solutions of CHL, eucalyptus oil (EO) and orange oil (OO) associated with CTR at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity by exposing treated bovine dentine blocks to E. faecalis. Biofilms grown in the dentine blocks for 7 days were exposed to solutions for 2 and 5 min. Biofilm reduction between OO and EO at 2 min did not show any significant differences; however, OO had a higher kill percentage of biofilms than did the eucalyptus oil at 5 min (p < 0.01). Combinations with CTR at all concentrations achieved a 100% kill rate at 2 and 5 min. The association of CTR with solvent agents achieved the maximum antimicrobial activity against E. faecalis biofilms in dentine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Martos¹,

Ferrer-Luque²,

González-Rodrı́guez³

et al. 2013

European Journal of Microbiology and Immunology

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“…1 It is widely recognised that residual bacterial contamination of the canals is likely to lead to failure. [2][3][4] Therefore bacteria remaining within a canal after chemo-mechanical preparation must be reduced to a minimum for successful treatment.…”

Section: Introductionmentioning

confidence: 99%

An alternative regimen for root canal disinfection

et al. 2006

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Objective To compare the effect of a combination of 20% citric acid solution and photo-activated disinfection with the use of 20% citric acid and 2.25% sodium hypochlorite solutions on bacterial load on the dentine walls in prepared canals in vivo. Subjects and methods Sixty-four randomly selected cases were evaluated and allocated to one of two groups. In Group 1, after gaining access to the root canal, bacterial load on the canal walls was sampled using endodontic files. A further sample was taken after apex location and initial widening of the canal had been completed and the photoactivated disinfection process carried out. A final sample was taken after completion of the canal preparation using citric acid and sodium hypochlorite solutions. In Group 2, the initial sample was taken as described previously. A second sample was taken after conventional preparation using 20% citric acid and sodium hypochlorite solutions as co-irrigants. A final sample was then taken after a subsequent PAD treatment. All samples were cultured for facultative anaerobic bacteria. Results Of the canals treated in Group 1 only two of the 23 canals infected showed culturable bacteria after the use of citric acid and photo-activated disinfection. Of these two canals, one was free of culturable bacteria on completion of conventional treatment but the other still contained culturable bacteria. In Group 2, four canals of the 23 infected initially, remained contaminated after conventional treatment. After subsequent photo-activated disinfection three of these four canals were free of culturable bacteria. Conclusion Results indicate that the use of a chelating agent acting as a cleaner and disrupter of the biofilm and photo-activated disinfection to kill bacteria is an effective alternative to the use of hypochlorite as a root canal cleaning system.

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“…12 There are numerous reports on the presence of Enterococcus faecalis in failed endodontic treatments. 2,13 The prognosis of the endodontic retreatment is significantly lower than the endodontic initial therapy due to more resistant microorganisms. Various adjuvant techniques have been used to overcome the problem.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

et al. 2016

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IntroductionMicrobial infection plays an important role in persistent periapical lesions.1 Insufficient disinfection of root canals could lead to treatment failure and persistent periapical pathology. 2Conventional techniques of root canal treatments such as mechanical instrumentation and chemical debridement with antimicrobial irrigants, such as sodium hypochlorite (NaOCl), chlorhexidine and calcium hydroxide do not always suffice to predictably render root canals free of bacteria.3,4 Factors such as anatomical complexities, bacterial growth as biofilm, render complete disinfection of the root canal system almost impossible. 5Studies have revealed a success rate of 94% with a negative culture before obturation has decreased to 68%, with a positive culture.2 Resolution of periapical lesions is more achievable when there is negative culture before obturation. 6Cross sectional studies have reported 20%-60% of treatment failure and 52% of endodontic failure in Iranian population.7-10 Existence of intracanal microorganisms would lessen the treatment outcomes of endodontic re-treatment comparing to primary endodontic treatment. 2Based on the previous studies, bacterial microflora in teeth with failed endodontic treatment is different from the microflora of the primary endodontic infection. All culture and PCR based methods have shown that primary endodontic infection is a polymicrobial infection involving fully anaerobic microorganisms, 5,11 while the failure AbstractIntroduction: The aim of this study was to compare the antibacterial efficacy of diode laser 810nm and photodynamic therapy (PDT) in reducing bacterial microflora in endodontic retreatment of teeth with periradicular lesion. Methods: In this in vivo clinical trial, 20 patients who needed endodontic retreatment were selected. After conventional chemo mechanical preparation of root canals, microbiological samples were taken with sterile paper point (PP), held in thioglycollate broth, and then were transferred to the microbiological lab. In the first group, PDT with methylene blue (MB) and diode laser (810 nm, 0.2 W, 40 seconds) was performed and in the second group diode laser (810 nm, 1.2 W, 30 seconds) was irradiated. Then second samples were taken from all canals. Results: CFU/ml amounts showed statistically significant reduction in both groups (P < 0.001). CFU/ml amounts were compared between the two groups and there was no statistical difference. Conclusion: PDT and diode laser 810 nm irradiation are effective methods for root canal disinfection. PDT is a suitable alternative for diode laser 810 nm irradiation, because of lower thermal risk on root dentin.

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Bacteria isolated after unsuccessful endodontic treatment in a North American population

Cited by 357 publications

References 24 publications

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

An alternative regimen for root canal disinfection

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

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