Bacteria from the rhizosphere and tissues of As-hyperaccumulator Pteris vittata and their role in arsenic transformation

Han, Yong-He; Jia, Meng-Ru; Li, Xue; Zhu, Ying Fang; Cao, Yue; Chen, Denglong; Chen, Yanshan; Lena, Q.

doi:10.1016/j.chemosphere.2017.08.031

Cited by 22 publications

(1 citation statement)

References 31 publications

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“…Studies show that the rhizosphere microbes of P. vittata play a crucial role in its As hyperaccumulation. − Among them, an arbuscular mycorrhizal fungus (AMF) effectively enhances As accumulation in P. vittata. − Some scientists believe that AMF can directly enhance As uptake and translocation by P.…”

Section: Introductionmentioning

confidence: 99%

Novel Mycorrhiza-Specific P Transporter PvPht1;6 Contributes to As Accumulation at the Symbiotic Interface of As-Hyperaccumulator Pteris vittata

Sun

Zhang

Liao

et al. 2022

Environ. Sci. Technol.

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Arsenic (As) is toxic and ubiquitous in the environment, posing a growing threat to human health. As-hyperaccumulator Pteris vittata has been used for phytoremediation of As-contaminated soil. Symbiosis with arbuscular mycorrhizal fungi (AMF) enhances As accumulation by P. vittata, which is different from As inhibition in typical plants. In this study, P. vittata seedlings inoculated with or without AMF were cultivated in As-contaminated soils for 2 months. AMF−root symbiosis enhanced plant growth, with 64.5% greater As contents in the fronds. After exposure to AsV for 2 h, the arsenate (AsV) and arsenite (AsIII) contents in AMF−roots increased by 1.8-and 3.6-fold, suggesting more efficient As uptake by P. vittata with AMF−roots. Plants take up and transport AsV via phosphate transporters (Phts). Here, for the first time, we identified a novel mycorrhiza-specific Pht transporter, PvPht1;6, from P. vittata. The transcripts of PvPht1;6 were strongly induced in AMF−roots, which were localized to the plasma membrane of arbuscule-containing cells. By complementing a yeast mutant lacking 5-Phts, we confirmed PvPht1;6′s transport activity for both P and AsV. In contrast to typical AMF-inducible phosphate transporter LePT4 from tomato, PvPht1;6 showed greater AsV transport capacity. The results suggest that PvPht1;6 is probably critical for AsV transport at the periarbuscular membrane of P. vittata root cells, revealing the underlying mechanism of efficient As accumulation in P. vittata with AMF−roots.

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Section: Introductionmentioning

confidence: 99%

Novel Mycorrhiza-Specific P Transporter PvPht1;6 Contributes to As Accumulation at the Symbiotic Interface of As-Hyperaccumulator Pteris vittata

Sun

Zhang

Liao

et al. 2022

Environ. Sci. Technol.

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Role of Plant-Microorganism Interactions in Plant Tolerance to Arsenic

Kowalczyk

Latowski

2018

Mechanisms of Arsenic Toxicity and Tolerance in Plants

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Mechanism of arsenic resistance in endophytic bacteria isolated from endemic plant of mine tailings and their arsenophore production

et al. 2018

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Arsenic contamination is an important environmental problem around the world since its high toxicity, and bacteria resist to this element serve as valuable resource for its bioremediation. Aiming at searching the arsenic-resistant bacteria and determining their resistant mechanism, a total of 27 strains isolated from roots of Prosopis laevigata and Spharealcea angustifolia grown in a heavy metal-contaminated region in Mexico were investigated. The minimum inhibitory concentration (MIC) and transformation abilities of arsenate (As) and arsenite (As), arsenophore synthesis, arsenate uptake, and cytoplasmatic arsenate reductase (arsC), and arsenite transporter (arsB) genes were studied for these strains. Based on these results and the 16S rDNA sequence analysis, these isolates were identified as arsenic-resistant endophytic bacteria (AREB) belonging to the genera Arthrobacter, Bacillus, Brevibacterium, Kocuria, Microbacterium, Micrococcus, Pseudomonas, and Staphylococcus. They could tolerate high concentrations of arsenic with MIC from 20 to > 100 mM for As and 10-20 mM for As. Eleven isolates presented dual abilities of As reduction and As oxidation. As the most effective strains, Micrococcus luteus NE2E1 reduced 94% of the As and Pseudomonas zhaodongensis NM2E7 oxidized 46% of As under aerobic condition. About 70 and 44% of the test strains produced arsenophores to chelate As and As, respectively. The AREB may absorb arsenate via the same receptor of phosphate uptake or via other way in some case. The cytoplasmic arsenate reductase and alternative arsenate reduction pathways exist in these AREB. Therefore, these AREB could be candidates for the bioremediation process.

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Bacteria from the rhizosphere and tissues of As-hyperaccumulator Pteris vittata and their role in arsenic transformation

Cited by 22 publications

References 31 publications

Novel Mycorrhiza-Specific P Transporter PvPht1;6 Contributes to As Accumulation at the Symbiotic Interface of As-Hyperaccumulator Pteris vittata

Novel Mycorrhiza-Specific P Transporter PvPht1;6 Contributes to As Accumulation at the Symbiotic Interface of As-Hyperaccumulator Pteris vittata

Role of Plant-Microorganism Interactions in Plant Tolerance to Arsenic

Mechanism of arsenic resistance in endophytic bacteria isolated from endemic plant of mine tailings and their arsenophore production

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