Bacteria from nodules of wild legume species: Phylogenetic diversity, plant growth promotion abilities and osmotolerance

Cardoso, Paulo; Alves, Artur; Silveira, Paulo; Sá, Carina; Fidalgo, Cátia; Freitas, Rosa; Figueira, Etelvina

doi:10.1016/j.scitotenv.2018.06.399

Cited by 51 publications

(37 citation statements)

References 43 publications

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“…Moreover, Pseudomonas was the only genus common to all legume species which agrees with the fact that Pseudomonas is one of the most dominant genera in plant microbiomes [59]. In addition, a recent study of native legume species in Portugal also revealed the predominance of this genus in the legume root nodules [60]. However, the bacterial diversity found in legume root nodules is considerably different from the diversity found in the legume roots studied herein.…”

Section: Discussionsupporting

confidence: 79%

“…In fact, a clear differentiation between non-rhizobial bacterial community composition in legume roots and nodules were previously observed in Lotus japonicus [62], soybean and alfalfa [56]. For instance, several genera, like Azospirillum , Flavobacterium , Lysobacter , Variovorax , Agrobacterium , Microvirga, and Phyllobacterium [60,63,64,65,66,67] that are typically found in root nodules from distinct legumes species were not detected in f the roots of the legume species studied here.…”

Section: Discussionmentioning

confidence: 93%

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Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

et al. 2019

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Bacterial endophytes, a subset of a plant’s microbiota, can facilitate plant growth by a number of different mechanisms. The aims of this study were to assess the diversity and functionality of endophytic bacterial strains from internal root tissues of native legume species grown in two distinct sites in South of Portugal and to evaluate their ability to promote plant growth. Here, 122 endophytic bacterial isolates were obtained from 12 different native legume species. Most of these bacteria possess at least one of the plant growth-promoting features tested in vitro, with indole acetic acid production being the most common feature among the isolates followed by the production of siderophores and inorganic phosphate solubilization. The results of in planta experiments revealed that co-inoculation of chickpea plants with specific endophytic bacteria along with N2-fixing symbionts significantly improved the total biomass of chickpea plants, in particular when these plants were grown under saline conditions. Altogether, this study revealed that Mediterranean native legume species are a reservoir of plant growth-promoting bacteria, that are also tolerant to salinity and to toxic levels of Mn. Thus, these bacterial endophytes are well adapted to common constraints present in soils of this region which constitutes important factors to consider in the development of bacterial inoculants for stressful conditions in the Mediterranean region.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 93%

Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

et al. 2019

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“…Until recently, it was thought that legume nodules were only inhabited by rhizobia, according to the plant trait regarding host specificity. However, recent studies have shown a different picture where nodules from several legume species have a great diversity of microorganisms (Aserse et al, 2013;De Meyer et al, 2015;Leite et al, 2017;Martínez-Hidalgo and Hirsch, 2017;Trabelsi et al, 2017;Cardoso et al, 2018;Zhang et al, 2018). Hence, the microbial community structure and the role of most NRB in the plant/rhizobia symbiotic relationship are still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Bradyrhizobium as the Only Rhizobial Inhabitant of Mung Bean (Vigna radiata) Nodules in Tropical Soils: A Strategy Based on Microbiome for Improving Biological Nitrogen Fixation Using Bio-Products

et al. 2021

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The mung bean has a great potential under tropical conditions given its high content of grain protein. Additionally, its ability to benefit from biological nitrogen fixation (BNF) through association with native rhizobia inhabiting nodule microbiome provides most of the nitrogen independence on fertilizers. Soil microbial communities which are influenced by biogeographical factors and soil properties, represent a source of rhizobacteria capable of stimulating plant growth. The objective of this study is to support selection of beneficial bacteria that form positive interactions with mung bean plants cultivated in tropical soils, as part of a seed inoculation program for increasing grain yield based on the BNF and other mechanisms. Two mung bean genotypes (Camaleão and Esmeralda) were cultivated in 10 soil samples. Nodule microbiome was characterized by next-generation sequencing using Illumina MiSeq 16S rRNA. More than 99% of nodule sequences showed similarity with Bradyrhizobium genus, the only rhizobial present in nodules in our study. Higher bacterial diversity of soil samples collected in agribusiness areas (MW_MT-I, II or III) was associated with Esmeralda genotype, while an organic agroecosystem soil sample (SE_RJ-V) showed the highest bacterial diversity independent of genotype. Furthermore, OTUs close to Bradyrhizobium elkanii have dominated in all soil samples, except in the sample from the organic agroecosystem, where just B. japonicum was present. Bacterial community of mung bean nodules is mainly influenced by soil pH, K, Ca, and P. Besides a difference on nodule colonization by OTU sequences close to the Pseudomonas genus regarding the two genotypes was detected too. Although representing a small rate, around 0.1% of the total, Pseudomonas OTUs were only retrieved from nodules of Esmeralda genotype, suggesting a different trait regarding specificity between macro- and micro-symbionts. The microbiome analysis will guide the next steps in the development of an inoculant for mung bean aiming to promote plant growth and grain yield, composed either by an efficient Bradyrhizobium strain on its own or co-inoculated with a Pseudomonas strain. Considering the results achieved, the assessment of microbial ecology parameters is a potent coadjuvant capable to accelerate the inoculant development process and to improve the benefits to the crop by soil microorganisms.

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“…Endophytic bacteria with these beneficial activities are frequently found in legume tissue, including nodules [92][93][94]. The isolation of bacteria other than rhizobia from the interior of the legume nodules has been increasingly reported in precious years [28,[30][31][32][33]95]. Some plant species seem more prone to harbor a high amounts and diversity of non-rhizobia inside their nodules, as reported by De Meyer et al [28], who studied 654 isolates from the nodules of 30 different plant species.…”

Section: Discussionmentioning

confidence: 96%

Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

et al. 2020

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Lotus spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few Lotus species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild Lotus parviflorus plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the Bradyrhizobium genus, which is closely related to B. canariense and other Bradyrhizobium sp. strains isolated from genistoid legumes and Ornithopus spp. Symbiotic nodC and nifH gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- (Rhizobium/Agrobacterium), beta- (Massilia) and gamma-proteobacteria (Pseudomonas, Lysobacter, Luteibacter, Stenotrophomonas and Rahnella), as well as some bacteroidetes from genera Sphingobacterium and Mucilaginibacter. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these L. parviflorus nodule-associated bacteria.

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Bacteria from nodules of wild legume species: Phylogenetic diversity, plant growth promotion abilities and osmotolerance

Cited by 51 publications

References 43 publications

Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

Bradyrhizobium as the Only Rhizobial Inhabitant of Mung Bean (Vigna radiata) Nodules in Tropical Soils: A Strategy Based on Microbiome for Improving Biological Nitrogen Fixation Using Bio-Products

Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

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