Bacteria-free solution derived from lactobacillus plantarum inhibits multiple NF-kappaB pathways and inhibits proteasome function

Petrof, Elaine O.; Claud, Erika C.; Sun, Jun; Abramova, Tatiana; Guo, Yanjie; Waypa, Tonya S.; He, Shikun; Nakagawa, Yasushi; Chang, Eugene B.

doi:10.1002/ibd.20930

Cited by 79 publications

(64 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some probiotic strains maintain or restore the epithelial barrier function after pathogen challenge (Otte and Podolsky 2004) or proinflammatory cytokine stimulus (Resta-Lenert and Barrett 2006), and others alter the capacity of intestinal epithelial cells (IEC) to release proinflammatory mediators or directly modify immune cell activity. Studies using IEC cultures have shown the downregulation of the secretion of the proinflammatory chemokine IL-8 (Frick et al 2007; Kamada et al 2008;Ma et al 2004;O'Hara et al 2006) and other NF-jB regulated chemokines, such as MCP-1 (Petrof et al 2009) or CCL-20 (Sibartie et al 2009), as well as a mechanism for the inhibition of IP-10 secretion. Also, a number of works demonstrated that probiotic bacteria directly interacting with immune cells elicit a differential cytokine production, thereby driving the polarization of T cells toward T h 1, T h 2, T h 17 or regulatory T cells (Foligne et al 2007a, b;Kekkonen et al 2008;Latvala et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

et al. 2012

View full text Add to dashboard Cite

Significant health benefits have been demonstrated for certain probiotic strains through intervention studies; however, there is a shortage of experimental evidence relative to the mechanisms of action. Here, noninvasive experimental procedure based on a colon organ culture system has been used that, in contrast to most experimental in vitro models reported, can preserve natural immunohistochemical features of the human mucosa. This system has been used to test whether commensal lactobacilli (Lactobacillus paracasei BL23, Lactobacillus plantarum 299v and L. plantarum 299v (A -)) were able to hinder inflammation-like signals induced by phorbol 12-myristate 13-acetate (PMA)/ionomycin (IO). Whole genome microarrays have been applied to analyze expression differences, from which mRNA markers could be inferred to monitor the effect of putative probiotic strains under such conditions. Regarding the gene expression, PMA/IO treatment induced not only interleukin (IL)-2 and interferon gamma (IFN-c), as expected, but also other relevant genes related to immune response and inflammation, such as IL-17A, chemokine (C-X-C motif) ligand (CXCL) 9 and CXCL11. The ex vivo culturing did not modify the pattern of expression of those genes or others related to inflammation. Interestingly, this study demonstrated that lactobacilli downregulated those genes and triggered a global change of the transcriptional profile that indicated a clear homeostasis restoring effect and a decrease in signals produced by activated T cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

et al. 2012

View full text Add to dashboard Cite

show abstract

“…These results highlight a strain and stimulus-dependent protection by LB, which we suggest is enhanced for L. plantarum and L. salivarious against NmB by a factor secreted in their conditioned medium (Figure 2). In our study inflammatory attenuation by their conditioned medium was largely independent of TLR-2 (Figure 3), in contrast to others [40], so is unlikely to involve LB lipoteichoic acid, which can be shed in vesicles by some strains [41] and is highly anti-inflammatory [42,43].…”

Section: T L Bekele Et Almentioning

confidence: 47%

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Bekele¹,

Keith²,

Adelina³

et al. 2014

AiM

View full text Add to dashboard Cite

In this study, we investigate the potential for oral Lactobacilli (LB) to afford innate protection against nasopharyngeal coloniser Neisseria meningitidis serogroup B (NmB), which causes the bulk of UK meningococcal disease. Oral isolates of L. plantarum, L. salivarious, L. casei, L. rhamnosus, L. gasseri and gut probiotic L. rhamnosus GG were assessed for their ability to suppress nasopharyngeal epithelial inflammatory responses to pathogenic NmB. The specificity of attenuation was examined using TLR 2 ligand, Pam3Cys, and early response cytokine IL1β; and the mechanism of attenuation was explored using heat-killed organisms and conditioned medium. Pro-inflammatory IL-6 and TNFα cytokine secretion was quantified by ELISA and associated cell death was quantified by PI staining and LDH release. NmB adhesion, invasion and metabolism were determined using standard gentamicin protection with viable counts, and bioluminescence, respectively. L. plantarum and L. salivarious suppressed IL-6 and TNFα secretions from NmB-infected epithelial cells. LB did not need to be alive and could suppress using secretions, which were independent of TLR2 or IL1β receptor signalling. L. plantarum, in particular, reduced NmB-induced necrotic cell death of epithelial monolayers. Like L. salivarious, it significantly inhibited NmB adhesion but uniquely L. plantarum abolished NmB invasion. Using bioluminescence as a reporter of pathogen metabolism, L. plantarum and its secretions were found to inhibit NmB metabolism during cell invasion assays. We conclude that oral L. plantarum and its secretions could be used to help reduce the burden of meningococcal disease by removing the intracellular nasopharyngeal reservoir of NmB.

show abstract

“…16,[30][31][32] Probiotic anaerobes have also been found to secrete anti-inflammatory agents. 25,33,34) Furthermore, our previous study showed that the flavonols quercetin, galangin, and fisetin enhance the production of NO suppressants by B. adolescentis. 25) However, whether these effects are distinct to flavonols and evoked by a characteristic structure of these compounds have not yet been clarified.…”

Section: Discussionmentioning

confidence: 99%

Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis

Kawabata

Kato

Sakano

et al. 2015

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Probiotics have been shown to improve the condition of not only the human gastrointestinal tract but also the entire body. We found that quercetin enhances the anti-inflammatory activity of Bifidobacterium adolescentis, which is abundant in human intestines. Here, we assessed whether certain phytochemicals could enhance the anti-inflammatory activity of B. adolescentis. Bifidobacteria were anaerobically cultured with phytochemicals for 3 h, and the anti-inflammatory activity of the supernatants was estimated by testing their ability to inhibit nitric oxide (NO) production by lipopolysaccharide-stimulated RAW264 macrophages. Of the 55 phytochemicals tested, phloretin, (+)-taxifolin, and (−)-epigallocatechin gallate as well as quercetin-3-O-glucoside and quercetin-4′-O-glucoside were similar to quercetin in promoting NO suppression by B. adolescentis. In addition, the phytochemicals excluding quercetin increased the concentrations of lactic and acetic acids in the co-culture supernatants. These results suggest that some phytochemicals may activate the anti-inflammatory function of B. adolescentis.

show abstract

Bacteria-free solution derived from lactobacillus plantarum inhibits multiple NF-kappaB pathways and inhibits proteasome function

Cited by 79 publications

References 49 publications

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis

Contact Info

Product

Resources

About

Bacteria-free solution derived from lactobacillus plantarum inhibits multiple NF-kappaB pathways and inhibits proteasome function

Cited by 79 publications

References 49 publications

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Oral &lt;i&gt;Lactobacillus plantarum&lt;/i&gt; NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of &lt;i&gt;Neisseria meningitidis&lt;/i&gt; Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis

Contact Info

Product

Resources

About

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells