Bacteria display optimal transport near surfaces

Ipiña, Emiliano Pérez; Otte, Stefan; Pontier-Bres, Rodolphe; Czerucka, Dorota; Peruani, Fernando

doi:10.1038/s41567-019-0460-5

Cited by 93 publications

(64 citation statements)

References 46 publications

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“…Similar running and lingering phases for cells near surface motion has also been reported in enterohaemorrhagic E. coli (EHEC) cells ( Perez Ipiña et al, 2019 ), where results suggested that by choosing the optimal transition rates, EHEC bacterial diffusivity is maximized and the surface exploration efficiency is greatly improved. In a future work, it will be interesting to apply similar analysis in V. cholerae .…”

Section: Discussionsupporting

confidence: 57%

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Zhang

Luo

Feng

et al. 2020

Preprint

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AbstractMannose-sensitive hemagglutinin (MSHA) pili and flagellum are critical for the surface attachment of Vibrio cholerae. However, the cell landing mechanism remains largely unknown. Here, combining the cysteine-substitution-based labelling method with single-cell tracking techniques, we quantitatively characterized the landing of V. cholerae by directly observing both pili and flagellum of cells in viscous solutions. MSHA pili are evenly distributed along the cell length and can stick to surfaces at any point along the filament. With such properties, MSHA pili are observed to act as a brake and anchor during cell landing which include three phases: running, lingering, and attaching. Resistive-force-theory based models are proposed to describe near-surface motion. Importantly, the role of MSHA pili during cell landing is more apparent in viscous solutions. Our work provides a detailed picture of the landing dynamics of V. cholerae under viscous conditions, which can provide insights into ways to better control V. cholerae infections.

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Section: Discussionsupporting

confidence: 57%

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Zhang

Luo

Feng

et al. 2020

Preprint

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“…Interestingly, for E. coli cells, as a consequence of a hydrodynamic torque, forwardscattering events on the obstacles also lead the cells' trajectory to leave the surface. Along with the intermittent motion shown by some pathogenic strains of E. coli near a flat surface [48], this behaviour can thus offer a way to potentially reduce escape times when swimming near it and maximise near-surface diffusivity [23][24][25][26]. As our study focused on flat surfaces, promising future directions include testing the robustness of the identified forward-scattering mechanism on curved surfaces (where the surface curvature varies on a length scale compa-rable to the cells' persistence length), near interfaces in the presence of floating obstacles as well as in 3D porous structures.…”

Section: Discussionmentioning

confidence: 99%

Enhanced propagation of motile bacteria on surfaces due to forward scattering

et al. 2019

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How motile bacteria move near a surface is a problem of fundamental biophysical interest and is key to the emergence of several phenomena of biological, ecological and medical relevance, including biofilm formation. Solid boundaries can strongly influence a cell’s propulsion mechanism, thus leading many flagellated bacteria to describe long circular trajectories stably entrapped by the surface. Experimental studies on near-surface bacterial motility have, however, neglected the fact that real environments have typical microstructures varying on the scale of the cells’ motion. Here, we show that micro-obstacles influence the propagation of peritrichously flagellated bacteria on a flat surface in a non-monotonic way. Instead of hindering it, an optimal, relatively low obstacle density can significantly enhance cells’ propagation on surfaces due to individual forward-scattering events. This finding provides insight on the emerging dynamics of chiral active matter in complex environments and inspires possible routes to control microbial ecology in natural habitats.

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“…Processing of data using artificial intelligence can automate the mapping of bacterial density along the root (Carbone et al, 2017). The ability to track bacteria has drastically improved since the early work of Shimshick and Hebert (1979), for example, observation of single bacterial cell and visualization of their attachment is now routinely achieved with modern microscopes (Duvernoy et al, 2018;Ipina et al, 2019). Mathematical frameworks will be essential to interpret such complex experimental data because they can establish links between attachment rates, root growth, bacterial proliferation, and the complex distribution of bacterial density along the root (Dupuy and Silk, 2016).…”

Section: Application Of Mathematical Framework For Estimation Of Attamentioning

confidence: 99%

Framework for Quantification of the Dynamics of Root Colonization by Pseudomonas fluorescens Isolate SBW25

et al. 2020

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Colonization of the root surface, or rhizoplane, is one of the first steps for soilborne bacteria to become established in the plant microbiome. However, the relative contributions of processes, such as bacterial attachment and proliferation is not well characterized, and this limits our ability to comprehend the complex dynamics of microbial communities in the rhizosphere. The work presented here addresses this knowledge gap. A model system was developed to acquire quantitative data on the colonization process of lettuce (Lactuca sativa L. cultivar. All Year Round) roots by Pseudomonas fluorescens isolate SBW25. A theoretical framework is proposed to calculate attachment rate and quantify the relative contribution of bacterial attachment to colonization. This allows the assessment of attachment rates on the root surface beyond the short time period during which it can be quantified experimentally. All techniques proposed are generic and similar analyses could be applied to study various combinations of plants and bacteria, or to assess competition between species. In the future this could allow for selection of microbial traits that improve early colonization and maintenance of targeted isolates in cropping systems, with potential applications for the development of biological fertilizers.

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Bacteria display optimal transport near surfaces

Cited by 93 publications

References 46 publications

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Enhanced propagation of motile bacteria on surfaces due to forward scattering

Framework for Quantification of the Dynamics of Root Colonization by Pseudomonas fluorescens Isolate SBW25

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