Bacteria Bacillus velezensis BIM В-439 D as the Basis of Biopreparation Betaprotectin to Control Root Rots of Cucumber

Abstract

Molecular-genetic and functional characterization of bacterial strain B. velezensis BIM В-439 D as the basis of biopesticide Betaprotectin was carried out. Bacterial genome contained 10 loci determining synthesis of polyfunctional enzyme complexes responsible for production of several antimicrobial compounds and lacking genes of drug resistance and pathogenicity. It was shown that the microbial culture suppressed development of a broad spectrum of fungal and bacterial pathogens infecting agricultural crops, raised germination power and sprouting rate of cucumber seeds, displayed high survival rate in natural soil microbiocenoses accounting for elevated phytoprotective and growth-stimulating potential and attractive application prospects as the active principle of biopesticide Betaprotectin. Prolonged application record of biopesticide throughout 10-year vegetation period to promote greenhouse cucumber culture provided for stable phytosanitary control of root rots, caused favorable effect on plant status and increased yields of vegetable products.