Bacteria at Work – Experimental and Theoretical Studies Reveal the Catalytic Mechanism of Ectoine Synthase
Justyna Andrys‐Olek,
Anna Kluza,
Mateusz Tataruch
et al.
Abstract:Ectoine synthase (EctC) catalyses the ultimate step of ectoine biosynthesis, a kosmotropic compound produced as compatible solute by many bacteria and some archaea or eukaryotes. EctC is an Fe2+‐dependent homodimeric cytoplasmic protein. Using Mössbauer spectroscopy, molecular dynamics simulations and QM/MM calculations, we determined the most likely coordination number and geometry of the Fe2+ ion and proposed a mechanism of the EctC‐catalysed reaction. Most notably, we show that apart from the three amino ac… Show more
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