Bacteria associated with sabellids (Polychaeta: Annelida) as a novel source of surface active compounds

Rizzo, Carmen; Michaud, Luigi; Hörmann, Barbara; Gerçe, Berna; Syldatk, Christoph; Hausmann, Rudolf; Domenico, Emilio De; Giudice, Angelina Lo

doi:10.1016/j.marpolbul.2013.02.020

Cited by 41 publications

(73 citation statements)

References 63 publications

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“…In fact, some biosurfactant producers gave a negative result to the haemolytic activity assay. This finding is in line with results previously obtained by other authors (Schulz et al 1991;Youssef et al 2004;Plaza et al 2006;Rizzo et al 2013) who reported the poor specificity of the method.…”

Section: Discussionsupporting

confidence: 93%

“…179 (E 24 & 37 % and &55 % in presence of tetradecane and sunflower oil, respectively), 181 (E 24 & 33 % and &57 in presence of tetradecane and sunflower oil, respectively) and 188 (E 24 & 3 % and &53 in presence of tetradecane and sunflower oil, respectively). This finding confirms the absence of a direct correlation between emulsifying and interfacial activity, as reported by Rizzo et al (2013) and could be dependent on different types of biosurfactants, by considering the existence of different pathways for their production. Moreover, surface active substances may have different interfacial capabilities: biosurfactant can be divided into low molecular mass molecules, which reduce surface tension at air-water interface, and high molecular mass polymers, which have a stronger emulsifying activity (Rosenberg and Ron 1999).…”

Section: Discussionsupporting

confidence: 90%

“…However, a unique study exists on the production of a polymeric emulsifying agent by a pseudoalteromonad under mesophilic conditions (Gutierrez et al 2008). Interestingly, we recently reported (Rizzo et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Single colonies grown on marine agar (MA) plates were lysed by thermal shock to extract genomic DNA. Cell lysates were directly used for 16S rRNA gene amplification by PCR (Rizzo et al 2013).…”

Section: Biomolecular Characterization Of Bs-producing Bacterial Isolmentioning

confidence: 99%

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Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

et al. 2015

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The risk of hydrocarbon contamination in marine polar areas is constantly increasing. Autochthonous bacteria, due to their ability to cope and survive under extreme environmental conditions, can play a fundamental role in the hydrocarbon degradation. The degradation process is often enhanced by the production of biosurfactant molecules. The present study reports for the first time on the isolation of biosurfactant-producing bacteria from Arctic and Antarctic shoreline sediments. A total of 199 psychrotolerant bacterial isolates were obtained from hydrocarbonamended (with crude or diesel oil) microcosms. A total of 18 isolates were selected for their ability to grow in the presence of crude oil and produce biosurfactants, as it was revealed by the production of good E 24 values (C50 %) and/or reduction in the surface tension (under 30 mN/m). The positive response of the isolates to both tests suggests a possible production of biosurfactants with emulsifying and interfacial activities. Biosurfactant-producing isolates were mainly affiliated to the genera Rhodococcus (14 isolates), followed by Pseudomonas (two isolates), Pseudoalteromonas (one isolate) and Idiomarina (one isolate). Thin-layer chromatography of biosurfactant crude extracts revealed that the majority of the selected isolates were able to produce glycolipidic surfactants. Our results enlarge the knowledge, which is still poor and fragmentary, on biosurfactant producers from polar areas and indicate marine polar sediments as a source of bacteria with potential applications in the remediation of hydrocarbon-contaminated cold environments.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Biomolecular Characterization Of Bs-producing Bacterial Isolmentioning

confidence: 99%

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Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

et al. 2015

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“…Most biosurfactants of marine origin reported till dates are obtained from microorganisms isolated from contaminated seawater and sediment. However, we have recently highlighted the potential of sabellids (Polychaeta: Annelida) as a novel source of BS-producing bacteria, with most of them that belonged to genera whose ability to produce biosurfactants was never reported before (Rizzo et al 2013). Among them, Joostella strain A8 was isolated from crude oil enrichment cultures which were set up with homogenates of the polychaete Megalomma claparedei (Gravier, 1906) collected in the brackish Lake Faro (Messina, Italy), characterized by high concentrations of HM.…”

Section: Introductionmentioning

confidence: 99%

Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906)

et al. 2015

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The effect of heavy metals on the activity of biosurfactants produced by Joostella strain A8 from the polychaete Megalomma claparedei was investigated. Biosurfactant activity was first improved by evaluating the influence of abiotic parameters. Higher E(24) indices were achieved at 25 °C in mineral salt medium supplemented with 2 % glucose, 3 % sodium chloride (w/v) and 0.1 % ammonium chloride (w/v). Considerable surface tension reduction was never recorded. Heavy metal tolerance was preliminarily assayed by plate diffusion method resulting in the order of toxicity Cd > Cu > Zn. The activity of biosurfactants was then evaluated in the presence of heavy metals at different concentrations in liquid cultures that were incubated under optimal conditions for biosurfactant activity. The production of stable emulsions resulted generally higher in the presence of metals. These findings suggest that biosurfactant production could represent a bacterial adaptive strategy to defend cells from a stress condition derived from heavy metals in the bulk environment.

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Cobetia

Ventosa¹,

Arahal²

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Co.be′ti.a. N.L. fem. n. Cobetia named after A. B. Cobet, who originally described the type species as “ Arthrobacter marinus .” Proteobacteria / Gammaproteobacteria / Oceanospirillales / Halomonadaceae / Cobetia The genus Cobetia is classified within the family Halomonadaceae and the order Oceanospirillales , in the class Gammaproteobacteria . The cells stain Gram‐negative and are motile and non‐endospore‐forming rods. Colonies are cream, white, or light yellow pigmented. Chemoorganotrophic. Strictly aerobic. Catalase‐positive and oxidase‐negative. Optimal growth at 5–6.5% (w/v) NaCl, at pH 6.5–8.5 and 25–38°C. The predominant cellular fatty acids are C 16:1 ω7 c , C 16:0 , C 12:0 3‐OH, C 18:1 ω7 c , and C 17:0 cyclo. The DNA G + C content is 61.4–64.6 mol%. Currently, the genus includes five species: Cobetia marina (type species of the genus), Cobetia amphilecti , Cobetia crustatorum , Cobetia litoralis, and Cobetia pacifica . The strains of these species were isolated from marine environments, marine sediments, marine invertebrates, and fermented seafood. DNA G + C content (mol%): 61.4–64.6 according to wet‐lab methods, 57.3–63.2 in genomic sequences. Type species: Cobetia marina (Cobet et al. 1970) Arahal et al. 2002a VL88, emend. Romanenko et al. 2013.

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Bacteria associated with sabellids (Polychaeta: Annelida) as a novel source of surface active compounds

Cited by 41 publications

References 63 publications

Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906)

Cobetia

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