Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri

Martínez-Falcón, Ana Paola; Durbán, Ana; Latorre, Amparo; Antón, Josefa; Marcos-García, Ma Ángeles

doi:10.1371/journal.pone.0027443

Cited by 10 publications

(10 citation statements)

References 36 publications

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“…Similar to our findings, a previous study did not observe changes in bacterial communities in two species of syrphid flies that fed on different larval resources and concluded that the insect midgut might be selective for certain species of microbes [42]. That study also found Enterobacteriaceae (different genera from what were found in our study) were the predominant colonizers of larval guts, suggesting that the gut of insects might be conducive for the proliferation of this group of Bacteria .…”

Section: Discussionsupporting

confidence: 92%

Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance

et al. 2013

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Microbes are important for mosquito nutrition, growth, reproduction and control. In this study, we examined bacterial communities associated with larval mosquitoes and their habitats. Specifically, we characterized bacterial communities associated with late larval instars of the western encephalitis mosquito ( Culex tarsalis ), the submerged portions of two emergent macrophytes (California bulrush, Schoenoplectus californicus and alkali bulrush, Schoenoplectus maritimus ), and the associated water columns to investigate potential differential use of resources by mosquitoes in different wetland habitats. Using next-generation sequence data from 16S rRNA gene hypervariable regions, the alpha diversity of mosquito gut microbial communities did not differ between pond mesocosms containing distinct monotypic plants. Proteobacteria, dominated by the genus Thorsellia (Enterobacteriaceae), was the most abundant phylum recovered from C . tarsalis larvae. Approximately 49% of bacterial OTUs found in larval mosquitoes were identical to OTUs recovered from the water column and submerged portions of the two bulrushes. Plant and water samples were similar to one another, both being dominated by Actinobacteria, Bacteroidetes, Cyanobacteria , Proteobacteria and Verrucomicrobia phyla. Overall, the bacterial communities within C . tarsalis larvae were conserved and did not change across sampling dates and between two distinct plant habitats. Although Thorsellia spp. dominated mosquito gut communities, overlap of mosquito gut, plant and water-column OTUs likely reveal the effects of larval feeding. Future research will investigate the role of the key indicator groups of bacteria across the different developmental stages of this mosquito species.

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Section: Discussionsupporting

confidence: 92%

Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance

et al. 2013

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“…Within the family Enterobacteriaceae, we identified the bacterium Erwinia in the larvae, pupae, and adult female Qfly microbiome. Erwinia plays role in nitrogen fixation and helps insects to sustain themselves in environments with limited oxygen [77]. It is likely that these functions are required at all life stages in the Qfly, potentially explaining the consistency in their relative abundances across the dataset.…”

Section: Discussionmentioning

confidence: 99%

Microbiome of the Queensland Fruit Fly through Metamorphosis

et al. 2020

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Bactrocera tryoni (Froggatt) (Queensland fruit fly, or “Qfly”) is a highly polyphagous tephritid fruit fly and a serious economic pest in Australia. Qfly biology is intimately linked to the bacteria and fungi of its microbiome. While there are numerous studies of the microbiome in larvae and adults, the transition of the microbiome through the pupal stage remains unknown. To address this knowledge gap, we used high-throughput Next-Generation Sequencing (NGS) to examine microbial communities at each developmental stage in the Qfly life cycle, targeting the bacterial 16S rRNA and fungal ITS regions. We found that microbial communities were similar at the larval and pupal stage and were also similar between adult males and females, yet there were marked differences between the larval and adult stages. Specific bacterial and fungal taxa are present in the larvae and adults (fed hydrolyzed yeast with sugar) which is likely related to differences in nutritional biology of these life stages. We observed a significant abundance of the Acetobacteraceae at the family level, both in the larval and pupal stages. Conversely, Enterobacteriaceae was highly abundant (>80%) only in the adults. The majority of fungal taxa present in Qfly were yeasts or yeast-like fungi. In addition to elucidating changes in the microbiome through developmental stages, this study characterizes the Qfly microbiome present at the establishment of laboratory colonies as they enter the domestication process.

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“…Functions were not experimentally attributed to the taxa identified here, across the soil-plant continuum. However, previous knowledge suggested a wide range of potential functions from putative diazotrophes (Oxalobacteraceae and Enterobacteriaceae) (Martínez-Falcón et al, 2011), antifungal producers (Pseudonocardiaceae and Chitinophagaceae) (Basilio et al, 2003;Miranda et al, 2013), to methane-oxidizers and phenolic compounds degraders (Methylobacteriaceae and Caulobacteraceae) (Hinrichs et al, 2000;Flynn et al, 2013).…”

Section: Endophytes Continuity Along the Soil-plant Continuum And Thementioning

confidence: 99%

Diversity of endophytes across the soil-plant continuum for Atriplex spp. in arid environments

et al. 2015

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Endophytes are hypothesized to be transferred across the soil-plant continuum, suggesting both the transfers of endophytes from environment to plant and from plant to soil. To verify this hypothesis and to assess the role of locality, we evaluated the similarity of microbial communities commonly found both in soils and endophytic communities in three arid regions, i.e. the Jornada LTER (Long Term Ecological Research) site in New Mexico, USA, and the research station of Jordan University of Science and Technology (JUST) and Khanasri research station in Badia region of Jordan. Rhizosphere and non-rhizosphere soils, leaves and seeds of Atriplex spp. were sampled. Diversity and distribution of bacteria and fungi across the soil-plant continuums were assessed by tag-encoded FLX amplicon pyrosequencing and sequence alignment. Of the total bacterial OTUs (operational taxonomic units), 0.17% in Khanasri research station, 0.16% in research station of JUST, and 0.42% in Jornada LTER site were commonly found across all the plant and soil compartments. The same was true for fungi in two regions, i.e. 1.56% in research station of JUST and 0.86% in Jornada LTER site. However, in Khanasri research station, 12.08% of total fungi OTUs were found in at least one soil compartment and one plant compartment. Putative Arthrobacter, Sporosarcina, Cladosporium and members of Proteobacteria and Actinobacteria were found across all the soil-plant continuums. Ascomycota, mainly including Didymellaceae, Pleosporaceae and Davidiellaceae were present across all the soil-plant continuums. Microbial communities in two regions of Jordan were similar to each other, but both of them were different from the Jornada LTER site of USA. SIMPER (similarity percentage) analysis of bacterial and fungal taxa for both soil and endophyte communities revealed that dissimilarities of two bacterial genera (Arthrobacter and Sporosarcina) and two fungal genera (Cladosporium and Alternaria) are very high, so they play key roles in the soil-plant continuums. A weighed Pearson correlation analysis for the specific bacterial OTUs in the soil-plant continuums only showed high similarity between the two regions of Jordan. However, fungal groups showed higher similarities among all regions. This research supports the hypothesis of continuity of certain bacterial and fungal communities across the soil-plant continuums, and also explores the influences of plant species and geographic specificity on diversity and distribution of bacteria and fungi.

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Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri

Cited by 10 publications

References 36 publications

Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance

Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance

Microbiome of the Queensland Fruit Fly through Metamorphosis

Diversity of endophytes across the soil-plant continuum for Atriplex spp. in arid environments

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