Bacteria as DNA vaccine carriers for genetic immunization

Schoen, Christoph; Stritzker, Jochen; Goebel, Werner; Pilgrim, Sabine

doi:10.1016/j.ijmm.2004.03.001

Cited by 94 publications

(84 citation statements)

References 123 publications

(116 reference statements)

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“…In addition, Salmonella provide ligands for toll-like receptors or ''danger signals,'' which can induce DC activation and maturation. The genes encoded by vectors can be then transcribed, translated, expressed, and processed by DCs (30). Here, in fact, we demonstrate the in vivo expression of H60 or CEA encoded by our DNA vaccine in DCs located inside Peyer's patches, proving the efficiency of this delivery system.…”

Section: Discussionmentioning

confidence: 58%

DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor

Zhou

Luo

Lo³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The interaction of NKG2D, a stimulatory receptor expressed on natural killer (NK) cells and activated CD8 ؉ T cells, and its ligands mediates stimulatory and costimulatory signals to these cells. Here, we demonstrate that DNA-based vaccines, encoding syngeneic or allogeneic NKG2D ligands together with tumor antigens such as survivin or carcinoembryonic antigen, markedly activate both innate and adaptive antitumor immunity. Such vaccines result in highly effective, NK؊ and CD8 ؉ T cell-mediated protection against either breast or colon carcinoma cells in prophylactic and therapeutic settings. Notably, this protection was irrespective of the NKG2D ligand expression level of the tumor cells. Hence, this strategy has the potential to lead to widely applicable and possibly clinically useful DNA-based cancer vaccines.NK cells ͉ NKG2D ligands ͉ T cells ͉ survivin ͉ Peyer's patches

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Section: Discussionmentioning

confidence: 58%

DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor

Zhou

Luo

Lo³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Recombinant Lm has great potential as a vaccine vehicle (10,11,25). Importantly for neonatal vaccination, recombinant attenuated strains of Lm induce Ag-specific immunity even in the presence of preexisting immunity (10), an issue of importance for vaccines where preexisting maternal immunity might interfere with the vaccine response in the newborn or infant.…”

Section: Discussionmentioning

confidence: 99%

Induction of Protective Immunity to Listeria monocytogenes in Neonates

Kollmann

Reikie

Blimkie

et al. 2007

The Journal of Immunology

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Neonates suffer unduly from infections and also respond suboptimally to most commonly used vaccines. However, a CD8 T cell response can be elicited in neonates if the Ag is introduced into the cytoplasm of APCs. Listeria monocytogenes (Lm) targets the cytoplasm of APC and is a strong CD8 and CD4 Th1-promoting vaccine vehicle in adult mice. We hypothesized that an attenuated strain of Lm would be safe and induce long-lasting protective immunity, even in neonates. We found that neonatal mice immunized only once with the attenuated strain ΔactA-Lm developed robust primary and secondary CD8 and CD4 Th1 responses and were fully protected from lethal challenge with virulent wild-type Lm without the need for a booster immunization. Furthermore, ΔactA-Lm expressing a heterologous recombinant Ag induced a strong CD8 and Th1 memory response to that Ag. Based on these data, we propose that ΔactA-Lm or derivatives thereof might serve as a vaccine vehicle for neonatal immunization.

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“…After lysis of red blood cells (180 mM NH 4 Cl, 17 mM Na 2 EDTA) and several washes, splenocytes were resuspended in RPMI-10 (RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U penicillin, 100 mg ml À1 streptomycin). Cells were incubated for 60 h at 37 1C (5% CO 2 ) in 96-well culture plates (10 6 cells per well) in the presence of BLG (20 mg ml À1 ) or concanavalin A (1 mg ml À1 , positive control).…”

Section: Cytokine Productionsmentioning

confidence: 99%

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

Pothelune

Ah‐Leung

et al. 2008

Gene Ther

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We recently demonstrated that noninvasive food-grade Lactococcus lactis (L. lactis) can deliver eukaryotic expression plasmid in mammalian cells in vitro. Here, we evaluated, in vivo, whether a eukaryotic expression plasmid carried by lactococci can translocate to the epithelial cells of the intestinal membrane. The strain LL(pLIG:BLG1) carrying one plasmid containing a eukaryotic expression cassette encoding b-lactoglobulin (BLG), a major allergen of cow's milk, was orally administered by gavage to mice. BLG cDNA was detected in the epithelial membrane of the small intestine of 40% of the mice and BLG was produced in 53% of the mice. Oral administration of LL(pLIG:BLG1) induced a low and transitory Th1-type immune response counteracting a Th2 response in case of further sensitization. We demonstrated for the first time the transfer of a functional plasmid to the epithelial membrane of the small intestine in mice by noninvasive food-grade lactococci.

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Bacteria as DNA vaccine carriers for genetic immunization

Cited by 94 publications

References 123 publications

DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor

DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor

Induction of Protective Immunity to Listeria monocytogenes in Neonates

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

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