2015
DOI: 10.1016/j.fm.2015.01.017
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Bacteria and yeast microbiota in milk kefir grains from different Italian regions

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Cited by 210 publications
(176 citation statements)
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References 63 publications
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“…The following PCR conditions were used: 94°C for 2 min, 35 cycles of 95°C for 20 s, 56°C for 45 s, and 72°C for 5 min, with a final extension at 72°C for 7 min. The fungal community was studied by sequencing of the D1-D2 domain of the 26S rRNA gene amplifying a fragment of 540 bp using the primers NL-1 (5=-GCATATCAATAAGCGGAGGAAAAG-3=) and NL-4 (5=-GGTCCGTGTTTCAAGACGG-3=) (21) as recently reported (54). The 454 adapters were included in the forward primer, followed by a 10-bp sample-specific MID.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The following PCR conditions were used: 94°C for 2 min, 35 cycles of 95°C for 20 s, 56°C for 45 s, and 72°C for 5 min, with a final extension at 72°C for 7 min. The fungal community was studied by sequencing of the D1-D2 domain of the 26S rRNA gene amplifying a fragment of 540 bp using the primers NL-1 (5=-GCATATCAATAAGCGGAGGAAAAG-3=) and NL-4 (5=-GGTCCGTGTTTCAAGACGG-3=) (21) as recently reported (54). The 454 adapters were included in the forward primer, followed by a 10-bp sample-specific MID.…”
Section: Methodsmentioning
confidence: 99%
“…PCR mixtures were prepared as described above. The following PCR conditions were used: 95°C for 5 min, followed by 35 cycles of 95°C for 1 min, 52°C for 45 s, and 72°C for 1 min, and then a final extension at 72°C for 7 min and holding at 4°C (54).…”
Section: Methodsmentioning
confidence: 99%
“…Since the OTU abundance is proportional to the number of reads obtained, this may lead to an incorrect estimation of OTU abundance (Bokulich and Mills, 2013b; Ercolini, 2013; De Filippis and Ercolini, 2016). Therefore, the use of different targets would be also advisable, such as the 26S (Garofalo et al ., 2015; Stellato et al ., 2015; Wang et al ., 2015) or the 18S rRNA (Liu et al ., 2015b; Minervini et al ., 2015) genes.…”
Section: Monitoring Microbes In Food Fermentationsmentioning
confidence: 99%
“…In the last decade, the introduction of newly developed sequencing strategies, commonly referred to as next-generation DNA sequencing techniques, has substantially revolutionised the study of genomics and molecular biology, being also successfully applied in food microbiology (Mayo et al 2014). However, the recent few studies exploiting and comparing high-throughput sequencing and PCR-DGGE surprisingly demonstrated how the latter technique still represents a valid tool for the profiling of complex food microbial communities (Delgado et al 2013;Garofalo et al 2015).…”
Section: Introductionmentioning
confidence: 99%