Bacopaside-I Alleviates the Detrimental Effects of Acute Paraquat Intoxication in the Adult Zebrafish Brain

Anand, Surendra Kumar; Sahu, Manas Ranjan; Mondal, Amal Chandra

doi:10.1007/s11064-021-03416-9

Cited by 5 publications

(6 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The zebrafish in the normal PBS-injected group were injected with 0.9% normal PBS and then injected with normal PBS again 30 min later. According to a previous study, a dose of 50 mg/kg is approximately the median lethal dose in zebrafish . Therefore, in our study, the zebrafish in the paraquat-injected group were euthanized at 0, 15, 30, 60, 240, 360, 480, 720, and 1440 min after the intraperitoneal injection of 50 mg/kg of paraquat solution.…”

Section: Materials and Methodsmentioning

confidence: 95%

“…The zebrafish in the normal PBS-injected group were injected with 0.9% normal PBS and then injected with normal PBS again 30 approximately the median lethal dose in zebrafish. 22 Therefore, in our study, the zebrafish in the paraquat-injected group were euthanized at 0, 15, 30, 60, 240, 360, 480, 720, and 1440 min after the intraperitoneal injection of 50 mg/kg of paraquat solution. The zebrafish in NaSAL treated after the paraquat injection group were injected with 50 mg/kg of paraquat solution and then were injected with 200 mg/kg of NaSAL solution after 30 min.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…29 The accumulation of paraquat in the brain of zebrafish is mainly related to its neurotoxicity, it is previously reported that the dopaminergic neurons can absorb paraquat through neutral amino acid transporters and induce oxidative stress-mediated neurotoxicity, and the oxidation− reduction imbalances lead to the death of dopaminergic neurons. 22 The gills of zebrafish are vital in various physiological functions, including respiration, excretion of nitrogen-contain- ing waste, osmotic regulation, ion regulation, and acid−base balance; they are the main sites of xenobiotic damage, where the toxicant metabolism and excretion occur. 30 The kidney, liver, and intestines are also the main metabolism-relevant organs of toxicants, and the liver also has a detoxification effect.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Qin,

Huang,

Feng

et al. 2024

Chem. Res. Toxicol.

View full text Add to dashboard Cite

Paraquat is a highly toxic quaternary ammonium herbicide. It can damage the functions of multiple organs and cause irreversible pulmonary fibrosis in the human body. However, the toxicological mechanism of paraquat is not yet fully understood, and due to the lack of specific antidotes, the clinical treatment of paraquat intoxication is still a great medical challenge. In-depth research on its toxicity mechanism, toxicokinetics, and effective antidotes is urgently demanded. A new molecular imaging technique, matrixassisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), can simultaneously achieve quantitative and spatial analysis and offer an alternative, distinct, and useful technique for paraquat intoxication and consequent detoxication. Here, we visualized the spatial-temporal distribution and conducted toxicokinetic research on paraquat in zebrafish by using stable isotope-labeled internal-standard-aided MALDI-MSI for the first time. The results indicated that paraquat had a fast absorption rate and was widely distributed in different organs, such as the brain, gills, kidneys, and liver in zebrafish. Its half-life was long, and the elimination rate was slow. Paraquat reached its peak at 30 min and was mainly distributed in kidneys and intestines and then showed a tendency of declining first but mildly rising later at 6 h, accompanied by a wide distribution in kidneys and intestines again. It suggested that entero-systemic recirculation might lead to the observed secondary peaks, and perhaps it extended the residence time of paraquat in the body. In addition, we validated the potential detoxification effect of sodium salicylate as a potential antidote for paraquat from both the dimensions of distribution and quantification. In conclusion, MALDI-MSI conveniently provided the distinct and quantitative spatial-temporal distribution information on paraquat in the whole body of zebrafish; it will promote the understanding of its toxicokinetic characteristics and provide more valuable information for clinical treatment.

show abstract

Section: Materials and Methodsmentioning

confidence: 95%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Qin,

Huang,

Feng

et al. 2024

Chem. Res. Toxicol.

View full text Add to dashboard Cite

show abstract

“…For instance, no lipid peroxidation was recently found in a zebrafish model for acute acrylamide neurotoxicity, despite the brain exhibited a total depletion of glutathione levels and a severely impaired thioredoxin system ( Raldúa et al, 2020 ). No lipid peroxidation was either found in the brain of zebrafish treated with paraquat at concentrations inducing 30–40% lethality, although the exposure to this herbicide resulted in a decrease in the superoxide dismutase (SOD) activity, catalase activity and GSH content ( Anand et al, 2021 ). However, in our study, only lipid peroxidation has been determined as indicator of oxidative stress, and the evaluation of the effects on the different components of the antioxidant defense system were out of the scope of the study.…”

Section: Discussionmentioning

confidence: 99%

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

et al. 2021

View full text Add to dashboard Cite

Hyperthermia is a common confounding factor for assessing the neurotoxic effects of methamphetamine (METH) in mammalian models. The development of new models of methamphetamine neurotoxicity using vertebrate poikilothermic animals should allow to overcome this problem. The aim of the present study was to develop a zebrafish model of neurotoxicity by binge-like methamphetamine exposure. After an initial testing at 20 and 40 mg/L for 48 h, the later METH concentration was selected for developing the model and the effects on the brain monoaminergic profile, locomotor, anxiety-like and social behaviors as well as on the expression of key genes of the catecholaminergic system were determined. A concentration- and time-dependent decrease in the brain levels of dopamine (DA), norepinephrine (NE) and serotonin (5-HT) was found in METH-exposed fish. A significant hyperactivity was found during the first hour of exposure, followed 3 h after by a positive geotaxis and negative scototaxis in the novel tank and in the light/dark paradigm, respectively. Moreover, the behavioral phenotype in the treated fish was consistent with social isolation. At transcriptional level, th1 and slc18a2 (vmat2) exhibited a significant increase after 3 h of exposure, whereas the expression of gfap, a marker of astroglial response to neuronal injury, was strongly increased after 48 h exposure. However, no evidences of oxidative stress were found in the brain of the treated fish. Altogether, this study demonstrates the suitability of the adult zebrafish as a model of METH-induced neurotoxicity and provides more information about the biochemical and behavioral consequences of METH abuse.

show abstract

“…Strategies for modeling PD in zebrafish have included genetic alterations that target proteins associated with PD, including pink1, − parkin, and others. − Another convenient strategy, which has been used in rodents primarily, is to chemically induce parkinsonism by treatment with a toxin either in the food or by injection . Toxins previously used in zebrafish for this purpose include 6-hydroxydopamine (6-OHDA), − 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), ,,,− paraquat, − and rotenone. − …”

Section: Introductionmentioning

confidence: 99%

Dopamine Release Impairments Accompany Locomotor and Cognitive Deficiencies in Rotenone-Treated Parkinson’s Disease Model Zebrafish

Hettiarachchi

Niyangoda

Jarošová

et al. 2022

Chem. Res. Toxicol.

View full text Add to dashboard Cite

In this work, we carried out neurochemical and behavioral analysis of zebrafish (Danio rerio) treated with rotenone, an agent used to chemically induce a syndrome resembling Parkinson's disease (PD). Dopamine release, measured with fast-scan cyclic voltammetry (FSCV) at carbon-fiber electrodes in acutely harvested whole brains, was about 30% of that found in controls. Uptake, represented by the first order rate constant (k) and the half-life (t 1/2 ) determined by nonlinear regression modeling of the stimulated release plots, was also diminished. Behavioral analysis revealed that rotenone treatment increased the time required for zebrafish to reach a reward within a maze by more than 50% and caused fish to select the wrong pathway, suggesting that latent learning was impaired. Additionally, zebrafish treated with rotenone suffered from diminished locomotor activity, swimming shorter distances with lower mean velocity and acceleration. Thus, the neurochemical and behavioral approaches, as applied, were able to resolve rotenone-induced differences in key parameters. This approach may be effective for screening therapies in this and other models of neurodegeneration.

show abstract

Bacopaside-I Alleviates the Detrimental Effects of Acute Paraquat Intoxication in the Adult Zebrafish Brain

Cited by 5 publications

References 76 publications

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

Dopamine Release Impairments Accompany Locomotor and Cognitive Deficiencies in Rotenone-Treated Parkinson’s Disease Model Zebrafish

Contact Info

Product

Resources

About