Backscattered electron imaging, X-ray microanalysis and X-ray microscopy in the study of cell death

Bowen, I. D.; Mullarkey, K.; Worrill, Nicola

doi:10.1016/0739-6260(92)90038-f

Cited by 4 publications

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“…Cytochemically, the levels of acid phos- phatase appear to peak in the histolytic pupal phase, but it is now clear that release of acid phosphatase from lysosomes is not a major contributory factor. Cytochemical results presented here confirm the view that a specific pattern of exoplasmic or free extracisternal acid phosphatase is generated de novo into cell death Ryder, 1974, 1976;Bowen et al, ,1982Bowen et al, ,1988Bowen, 1979,1993). The free hydrolase localized, first appears in association with ribosomes, and does not appear to diffuse from vacuolar sources.…”

Section: Discussionsupporting

confidence: 91%

“…Fine structural aspects of blow-fly larvae salivary gland metamorphosis were initially addressed by Levy (1984) and Levy and Bautz (1985), and further details are presented here along with the functional basis of this hormonally induced programmed cell death. In this report, particular attention will be paid to fine structural changes and changes in subcellular hydrolase patterns in Calliphora similar to those previously observed in Heliothis (Bowen et al, 1988) or cotton boll worm and Drosophih metamorphosis (Jones and Bowen, 1993).…”

Section: Introductionmentioning

confidence: 74%

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Programmed cell death during metamorphosis in the blow-flyCalliphora vomitoria

Bowen

Mullarkey

Morgan

1996

Microsc. Res. Tech.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 74%

Programmed cell death during metamorphosis in the blow-flyCalliphora vomitoria

Bowen

Mullarkey

Morgan

1996

Microsc. Res. Tech.

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“…These vesiculation phenomena have also been observed in leadexposed rat fibroblasts (Brun & Brunk, 1973). Severe lysosomal membrane destabilization resulting in leakage of lytic enzymes into the cytosol may initiate degenerative alterations and even cell death (De Duve 1969;Bowen et al, 1988). On the other hand, damage to the chloragosomal membranes together with accumulation of lead that results in displacing of calcium from its binding sites could result in release of cal- Fig.…”

Section: Discussionmentioning

confidence: 93%

The effect of sublethal lead exposure on the ultrastructure and on the distribution of acid phosphatase activity in chloragocytes of earthworms (Annelida, Oligochaeta)

et al. 1995

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Laboratory experiments were conducted to study the effects of the exposure to a sublethal concentration (500 p.p.m.) of lead on the ultrastructure and acid phosphatase compartmentalization of the chloragogenous tissue of earthworms, Eisenia foetida. For the cytochemical demonstration of acid phosphatase activity, lead and cerium were used as capturing agents. In both cases there was a change in the compartmentalization of acid phosphatase, the enzyme activity being localized within the chloragosomes in controls, but distributed throughout the cytosol in treated animals. In addition, acid phosphatase activity increased following lead exposure. At the ultrastructural level, disruption of the chloragosomal membranes, an increase in chloragosomal fusion processes and vesiculation of the cytoplasm were evident. Moreover, an enhanced release of chloragosomes to the extracellular space was found in lead-exposed worms.

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“…The molluscicide does however appear to considerably reduce the intensity of ATPase activity. This is not surprising since loss of ATP production and a decline in ATPase activity is symptomatic of necrosis (Bowen et al, 1988;Bowen and Bowen, 1990).…”

Section: Discussionmentioning

confidence: 99%

The histopathological effect of Detarium microcarpum extract, a naturally occurring plant molluscicide on the mid‐gut and digestive gland of Lymnaea stagnalis

Kela¹

1995

Cell Biology International

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The histology, fine structure and histochemistry of the digestive gland of Lymnaea stagnalis was examined before and after treatment with a crude water extract of a powerful molluscicidal plant, Detarium microcarpum. The results obtained indicate that the molluscicidal extract induces necrosis in the digestive gland typified optically by cellular oedaema and nuclear pyknosis. At electron microscope level molluscicide treatment is seen to induce a major vacuolation and fragmentation of the cytoplasm. Lysosomal acid phosphatase and lysosomal integrity however is preserved. Histochemically, both acid and alkaline phosphatase appear to survive treatment very well. In treated tissues there is a shift of alkaline phosphatase from the brush border to the lumen. Molluscicide treatment results in a distinct inhibition of ATPase activity which appears to be a characteristic feature of induced cell death.

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Backscattered electron imaging, X-ray microanalysis and X-ray microscopy in the study of cell death

Cited by 4 publications

References 0 publications

Programmed cell death during metamorphosis in the blow-flyCalliphora vomitoria

Programmed cell death during metamorphosis in the blow-flyCalliphora vomitoria

The effect of sublethal lead exposure on the ultrastructure and on the distribution of acid phosphatase activity in chloragocytes of earthworms (Annelida, Oligochaeta)

The histopathological effect of Detarium microcarpum extract, a naturally occurring plant molluscicide on the mid‐gut and digestive gland of Lymnaea stagnalis

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