Background Suppression in Imaging Gold Nanorods through Detection of Anti-Stokes Emission

Carattino, Aquiles; Keizer, Veer I. P.; Schaaf, Marcel J. M.; Orrit, Michel

doi:10.1016/j.bpj.2016.10.035

Cited by 16 publications

(24 citation statements)

References 43 publications

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“…We already exploited this phenomenon to image gold nanorods in high-background conditions. 43 For more information on the power dependence of both the anti-Stokes and Stokes luminescence, please refer to the Supporting Information .…”

Section: Resultsmentioning

confidence: 99%

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Gold Nanoparticles as Absolute Nanothermometers

2018

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Nanothermometry is a challenging field that can open the door to intriguing questions ranging from biology and medicine to material sciences. Gold nanorods are excellent candidates to act as nanoprobes because they are reasonably bright emitters upon excitation with a monochromatic source. Gold nanoparticles are commonly used in photothermal therapy as efficient transducers of electromagnetic radiation into heat. In this work we use the spectrum of the anti-Stokes emission from gold nanorods irradiated in resonance to measure the absolute temperature of the nanoparticles and their surrounding medium without the need for a previous calibration. We show a 4 K accuracy in the determination of the temperature of the medium with spectral measurements of 180 s integration time. This procedure can be easily implemented in any microscope capable of acquiring emission spectra, and it is not limited to any specific shape of nanoparticles.

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Section: Resultsmentioning

confidence: 99%

“…In most fluorescence detection schemes, however, these hot bands are ignored, but they are far from negligible in heavily doped samples. 43 …”

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confidence: 99%

Gold Nanoparticles as Absolute Nanothermometers

2018

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“…20 These results imply that the Fermi-Dirac distribution of the electrons in the sp band is the main factor influencing the spectral shape of light emission from plasmonic nanostructures. 10,21 In summary, light emission spectra of single gold nanorods was measured with the use of notch filters with ultra-narrow bandwidths. We confirmed that the Stokes emission spectral shape dominated by LSP resonance could be fitted by a Lorentzian line shape.…”

Section: Introductionmentioning

confidence: 99%

Spectral shape of one-photon luminescence from single gold nanorods

Wen

Liu

et al. 2017

AIP Advances

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Light emission from gold nanoparticles was investigated with ultra-narrow-band notch filters to obtain the complete spectral shape. The anti-Stokes emission band was observed at all excitation wavelengths. The spectral shape of the anti-Stokes emission could be well fitted by a Fermi–Dirac-like line shape, while the spectral profile of the Stokes emission could be fitted by a Lorentzian line shape. The electron distribution and local surface plasmon resonance jointly determined the spectral shape. Additionally, we found that the anti-Stokes emission intensity increased more rapidly compared with that of the Stokes emission as illumination power was increased. This phenomenon can be understood from the temperature dependence of the electron distribution owing to photothermal effects.

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“…It may in some cases be necessary to only use fluorescence and generate a very high precision overlay 19 20 . Alternatively, there is the promising possibility of using single gold particles as the marker for both LM and EM, by leveraging ICPM or Four Wave Mixing 21 as the light imaging modality or through detection of the luminescent blinking of gold nanoparticles 22 potentially with reduced background by using the anti-Stokes emission 23 . Although we find here that gold-conjugated fluorophores are not suitable fiducial markers, there is evidence that the length of the linker may affect quenching efficiency with a larger spacer potentially even enhancing fluorescence emission 24 25 .…”

Section: Resultsmentioning

confidence: 99%

Direct Evidence of Lack of Colocalisation of Fluorescently Labelled Gold Labels Used in Correlative Light Electron Microscopy

Miles

Greenwood

Benito‐Alifonso

et al. 2017

Sci Rep

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Fluorescently labelled nanoparticles are routinely used in Correlative Light Electron Microscopy (CLEM) to combine the capabilities of two separate microscope platforms: fluorescent light microscopy (LM) and electron microscopy (EM). The inherent assumption is that the fluorescent label observed under LM colocalises well with the electron dense nanoparticle observed in EM. Herein we show, by combining single molecule fluorescent imaging with optical detection of the scattering from single gold nanoparticles, that for a commercially produced sample of 10 nm gold nanoparticles tagged to Alexa-633 there is in fact no colocalisation between the fluorescent signatures of Alexa-633 and the scattering associated with the gold nanoparticle. This shows that the attached gold nanoparticle quenches the fluorescent signal by ~95%, or less likely that the complex has dissociated. In either scenario, the observed fluorescent signal in fact arises from a large population of untagged fluorophores; rendering these labels potentially ineffective and misleading to the field.

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Background Suppression in Imaging Gold Nanorods through Detection of Anti-Stokes Emission

Cited by 16 publications

References 43 publications

Gold Nanoparticles as Absolute Nanothermometers

Gold Nanoparticles as Absolute Nanothermometers

Spectral shape of one-photon luminescence from single gold nanorods

Direct Evidence of Lack of Colocalisation of Fluorescently Labelled Gold Labels Used in Correlative Light Electron Microscopy

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