Back transmission of apple chlorotic leafspot virus (type strain) to apple and production of apple topworking disease symptoms in Maruba Kaido (Malus prunifolia Borkh. var. ringo Asami.

Yanase, H.; Yamaguchi, A.; Mink, G. I.; Sawamura, Kenzo

doi:10.3186/jjphytopath.45.369

Cited by 13 publications

(9 citation statements)

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“…It generally has proved difficult to inoculate fruit trees, including apple, with viruses by conventional methods such as sap inoculation and stem slashing (Yanase et al 1979;Muruganantham et al 2009;Yamagishi et al 2010). We had recently developed an efficient method by biolistic inoculation of the cotyledons of seeds just after germination with viral RNAs by particle bombardment (Yamagishi The results presented here showed that the Arabidopsis FT gene expressed by the ALSV vector could induce early flowering in apple seedlings.…”

Section: Discussionmentioning

confidence: 98%

Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector

et al. 2010

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Tree crops have a long juvenile period which is a serious constraint for genetic improvement and experimental research. For example, apple remains in a juvenile phase for more than five years after seed germination. Here, we report about induction of rapid flowering in apple seedlings using the Apple latent spherical virus (ALSV) vector expressing a FLOWERING LOCUS T (FT) gene from Arabidopsis thaliana. Apple seedlings could be flowered at 1.5-2 months after inoculation to cotyledons of seeds just after germination with ALSV expressing the FT gene. A half of precocious flowers was normal in appearance with sepals, petals, stamens, and pistils. Pollen from a precocious flower successfully pollinated flowers of 'Fuji' apple from which fruits developed normally and next-generation seeds were produced. Our system using the ALSV vector promoted flowering time of apple seedlings within two months after germination and shortened the generation time from seed germination to next-generation seed maturation to within 7 months when pollen from precocious flowers was used for pollination.

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Section: Discussionmentioning

confidence: 98%

Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector

et al. 2010

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“…pitting virus (ASPV) can cause top-working disease of apple and pear in sensitive rootstocks (Yanase et al 1979;Desvignes and Boyé 1989). In stone-fruit trees the virus leads to severe symptoms, including dark green sunken mottle in peach, rosette formation and graft incompatibility in apricot, pseudopox and bark split in plum, and bark split in cherry (Dunez et al 1972;Desvignes and Boyé 1989;Cieślińska et al 1995;Jelkmann and Kunze 1995).…”

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confidence: 98%

Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China

et al. 2011

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Apple chlorotic leaf spot virus (ACLSV) isolates from sand pear (Pyrus pyrifolia) were characterized by analyzing the sequences of their coat protein (CP) genes and serological reactivity of recombinant coat proteins (rCPs). The sequences of CP genes from 22 sand pear isolates showed a high divergence, with 87.3-100% identities at the nucleotide (nt) level and 92.7-100% identities at the amino acid (aa) level. Phylogenetic analysis on the aa sequence of CP showed that the analyzed ACLSV isolates fell into different clusters and all isolates from sand pear were grouped into a large cluster (I) which was then divided into two sub-clusters (A and B). Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western blot and enzyme-linked immunosorbent assay (ELISA) analyses demonstrated that rCPs of eight ACLSV isolates (PP13, PP15-2, PP24, PP43, PE, PP54, PP56 and ACLSV-C) from two subclusters had different mobility rates and serological reactivity. The rCPs of five isolates grouped into the sub-cluster A showed stronger reactivity with antibodies against rCPs of a sand pear isolate ACLSV-BD and virions of a Japanese apple isolate P-205 than that with the antibody against a Chinese apple isolate ACLSV-C. Three isolates grouped into the sub-cluster B showed stronger reactivity with the antibody against ACLSV-C. The antigenic determinants of CPs from these eight isolates and isolates ACLSV-BD and P-205 were predicted. These results contribute to a further understanding of molecular diversity of the virus and its implication in serological detection.

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“…In contrast, only a few studies on virus vectors for VIGS in fruit tree species had been reported until recently, mainly because of the lack of efficient viral vectors. Furthermore, woody plants are usually more resistant to virus infections and the mechanical inoculation methods routinely used for herbaceous plants are not readily applicable to fruit tree species (Yamagishi et al, 2010;Yanase et al, 1979).…”

Section: Construction Of Alsv Vectorsmentioning

confidence: 99%

Virus-induced Gene Silencing in Apricot (Prunus armeniaca L.) and Japanese Apricot (P. mume Siebold ^|^amp; Zucc.) with the Apple Latent Spherical Virus Vector System

Kawai

Gonoi

Nitta

et al. 2014

J. Japan. Soc. Hort. Sci.

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Apple latent spherical virus (ALSV) vectors have been shown to effectively induce stable virus-induced gene silencing (VIGS) in a wide range of plant species, including rosaceous fruit tree species such as apple (Malus × domestica Borkh.), pear (Pyrus communis L.), and Japanese pear (P. pyrifolia Nakai). In this study, we attempted to develop a VIGS-based gene evaluation system for two Prunus fruit tree species, apricot and Japanese apricot, using ALSV vectors. A partial sequence of the P. armeniaca PHYTOENE DESATURASE (ParPDS) gene was cloned and ligated into the T-DNA region of a binary vector, pBICAL2, designed based on RNA2 of ALSV. The resultant pBICAL2-ParPDS was introduced into a disarmed Agrobacterium strain, EHA105. pBICAL1, a binary plasmid for the expression of ALSV RNA1 in plants, was also introduced into EHA105. Leaves of Nicotiana benthamiana were infected with pBICAL1/EHA105 and pBICAL2-ParPDS/EHA105 simultaneously to produce and amplify recombinant ALSV particles. The amplified ParPDS-ALSV in N. benthamiana was isolated and infected into the cotyledons of apricot and Japanese apricot seedlings by particle bombardment. Although our attempts to infect wild and recombinant ALSVs into Japanese apricot seedlings were unsuccessful, uniform discoloration of the upper leaves, a typical phenotype of PDS knock down, was observed several weeks after inoculation in apricot seedlings. We discuss the possible use of this VIGS-based gene evaluation system in Prunus.

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Back transmission of apple chlorotic leafspot virus (type strain) to apple and production of apple topworking disease symptoms in Maruba Kaido (Malus prunifolia Borkh. var. ringo Asami.

Cited by 13 publications

References 0 publications

Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector

Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector

Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China

Virus-induced Gene Silencing in Apricot (Prunus armeniaca L.) and Japanese Apricot (P. mume Siebold ^|^amp; Zucc.) with the Apple Latent Spherical Virus Vector System

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